Water-Soluble <i>Saccharina latissima</i> Polysaccharides and Relation of Their Structural Characteristics with <i>In Vitro</i> Immunostimulatory and Hypocholesterolemic Activities
Brown macroalgae are an important source of polysaccharides, mainly fucose-containing sulphated polysaccharides (FCSPs), associated with several biological activities. However, the structural diversity and structure–function relationships for their bioactivities are still undisclosed. Thus, the aim...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/3/183 |
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| author | Ana S. P. Moreira Diana Gaspar Sónia S. Ferreira Alexandra Correia Manuel Vilanova Marie-Mathilde Perrineau Philip D. Kerrison Claire M. M. Gachon Maria Rosário Domingues Manuel A. Coimbra Filipe M. Coreta-Gomes Cláudia Nunes |
| author_facet | Ana S. P. Moreira Diana Gaspar Sónia S. Ferreira Alexandra Correia Manuel Vilanova Marie-Mathilde Perrineau Philip D. Kerrison Claire M. M. Gachon Maria Rosário Domingues Manuel A. Coimbra Filipe M. Coreta-Gomes Cláudia Nunes |
| author_sort | Ana S. P. Moreira |
| collection | DOAJ |
| description | Brown macroalgae are an important source of polysaccharides, mainly fucose-containing sulphated polysaccharides (FCSPs), associated with several biological activities. However, the structural diversity and structure–function relationships for their bioactivities are still undisclosed. Thus, the aim of this work was to characterize the chemical structure of water-soluble <i>Saccharina latissima</i> polysaccharides and evaluate their immunostimulatory and hypocholesterolemic activities, helping to pinpoint a structure–activity relationship. Alginate, laminarans (F1, neutral glucose-rich polysaccharides), and two fractions (F2 and F3) of FCSPs (negatively charged) were studied. Whereas F2 is rich in uronic acids (45 mol%) and fucose (29 mol%), F3 is rich in fucose (59 mol%) and galactose (21 mol%). These two fractions of FCSPs showed immunostimulatory activity on B lymphocytes, which could be associated with the presence of sulphate groups. Only F2 exhibited a significant effect in reductions in <i>in vitro</i> cholesterol’s bioaccessibility attributed to the sequestration of bile salts. Therefore, <i>S. latissima</i> FCSPs were shown to have potential as immunostimulatory and hypocholesterolemic functional ingredients, where their content in uronic acids and sulphation seem to be relevant for the bioactive and healthy properties. |
| first_indexed | 2024-03-11T06:16:09Z |
| format | Article |
| id | doaj.art-4eeafba4b4f94f7fa2c8efe927493321 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T06:16:09Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-4eeafba4b4f94f7fa2c8efe9274933212023-11-17T12:17:42ZengMDPI AGMarine Drugs1660-33972023-03-0121318310.3390/md21030183Water-Soluble <i>Saccharina latissima</i> Polysaccharides and Relation of Their Structural Characteristics with <i>In Vitro</i> Immunostimulatory and Hypocholesterolemic ActivitiesAna S. P. Moreira0Diana Gaspar1Sónia S. Ferreira2Alexandra Correia3Manuel Vilanova4Marie-Mathilde Perrineau5Philip D. Kerrison6Claire M. M. Gachon7Maria Rosário Domingues8Manuel A. Coimbra9Filipe M. Coreta-Gomes10Cláudia Nunes11LAQV-REQUIMTE—Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, PortugalLAQV-REQUIMTE—Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, PortugalLAQV-REQUIMTE—Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugali3S—Institute for Research and Innovation in Health and IBMC—Institute for Molecular and Cell Biology, University of Porto, 4200-135 Porto, Portugali3S—Institute for Research and Innovation in Health and IBMC—Institute for Molecular and Cell Biology, University of Porto, 4200-135 Porto, PortugalScottish Association for Marine Sciences, Scottish Marine Institute, Oban PA37 1QA, UKScottish Association for Marine Sciences, Scottish Marine Institute, Oban PA37 1QA, UKScottish Association for Marine Sciences, Scottish Marine Institute, Oban PA37 1QA, UKLAQV-REQUIMTE—Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, PortugalLAQV-REQUIMTE—Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, PortugalLAQV-REQUIMTE—Associated Laboratory for Green Chemistry of the Network of Chemistry and Technology, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, PortugalCICECO—Aveiro Institute of Materials, Department of Materials and Ceramic Engineering, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, PortugalBrown macroalgae are an important source of polysaccharides, mainly fucose-containing sulphated polysaccharides (FCSPs), associated with several biological activities. However, the structural diversity and structure–function relationships for their bioactivities are still undisclosed. Thus, the aim of this work was to characterize the chemical structure of water-soluble <i>Saccharina latissima</i> polysaccharides and evaluate their immunostimulatory and hypocholesterolemic activities, helping to pinpoint a structure–activity relationship. Alginate, laminarans (F1, neutral glucose-rich polysaccharides), and two fractions (F2 and F3) of FCSPs (negatively charged) were studied. Whereas F2 is rich in uronic acids (45 mol%) and fucose (29 mol%), F3 is rich in fucose (59 mol%) and galactose (21 mol%). These two fractions of FCSPs showed immunostimulatory activity on B lymphocytes, which could be associated with the presence of sulphate groups. Only F2 exhibited a significant effect in reductions in <i>in vitro</i> cholesterol’s bioaccessibility attributed to the sequestration of bile salts. Therefore, <i>S. latissima</i> FCSPs were shown to have potential as immunostimulatory and hypocholesterolemic functional ingredients, where their content in uronic acids and sulphation seem to be relevant for the bioactive and healthy properties.https://www.mdpi.com/1660-3397/21/3/183seaweedLaminarialessulphated polysaccharidesfucansfucoidansmice |
| spellingShingle | Ana S. P. Moreira Diana Gaspar Sónia S. Ferreira Alexandra Correia Manuel Vilanova Marie-Mathilde Perrineau Philip D. Kerrison Claire M. M. Gachon Maria Rosário Domingues Manuel A. Coimbra Filipe M. Coreta-Gomes Cláudia Nunes Water-Soluble <i>Saccharina latissima</i> Polysaccharides and Relation of Their Structural Characteristics with <i>In Vitro</i> Immunostimulatory and Hypocholesterolemic Activities Marine Drugs seaweed Laminariales sulphated polysaccharides fucans fucoidans mice |
| title | Water-Soluble <i>Saccharina latissima</i> Polysaccharides and Relation of Their Structural Characteristics with <i>In Vitro</i> Immunostimulatory and Hypocholesterolemic Activities |
| title_full | Water-Soluble <i>Saccharina latissima</i> Polysaccharides and Relation of Their Structural Characteristics with <i>In Vitro</i> Immunostimulatory and Hypocholesterolemic Activities |
| title_fullStr | Water-Soluble <i>Saccharina latissima</i> Polysaccharides and Relation of Their Structural Characteristics with <i>In Vitro</i> Immunostimulatory and Hypocholesterolemic Activities |
| title_full_unstemmed | Water-Soluble <i>Saccharina latissima</i> Polysaccharides and Relation of Their Structural Characteristics with <i>In Vitro</i> Immunostimulatory and Hypocholesterolemic Activities |
| title_short | Water-Soluble <i>Saccharina latissima</i> Polysaccharides and Relation of Their Structural Characteristics with <i>In Vitro</i> Immunostimulatory and Hypocholesterolemic Activities |
| title_sort | water soluble i saccharina latissima i polysaccharides and relation of their structural characteristics with i in vitro i immunostimulatory and hypocholesterolemic activities |
| topic | seaweed Laminariales sulphated polysaccharides fucans fucoidans mice |
| url | https://www.mdpi.com/1660-3397/21/3/183 |
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