Analysis of Sugars in Honey Samples by Capillary Zone Electrophoresis Using Fluorescence Detection
The applicability of capillary electrophoresis (CE) with light-emitting diode-induced fluorescence detection (LEDIF) for the separation of sugars in honey samples was studied. An amount of 25 mM ammonium acetate (pH 4.5) with 0.3% polyethylene oxide (PEO) was found to be optimal for the efficient se...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Separations |
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| Online Access: | https://www.mdpi.com/2297-8739/10/3/150 |
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| author | Melinda Andrasi Gyongyi Gyemant Zsofi Sajtos Cynthia Nagy |
| author_facet | Melinda Andrasi Gyongyi Gyemant Zsofi Sajtos Cynthia Nagy |
| author_sort | Melinda Andrasi |
| collection | DOAJ |
| description | The applicability of capillary electrophoresis (CE) with light-emitting diode-induced fluorescence detection (LEDIF) for the separation of sugars in honey samples was studied. An amount of 25 mM ammonium acetate (pH 4.5) with 0.3% polyethylene oxide (PEO) was found to be optimal for the efficient separation of carbohydrates. 8-aminopyrene-1,3,6-trisulfonic acid (APTS) was used for the labeling of the carbohydrate standards and honey sugars for fluorescence detection. The optimized method was applied in the quantitative analysis of fructose and glucose by direct injection of honey samples. Apart from the labeling reaction, no other sample preparation was performed. The mean values of the fructose/glucose ratio for phacelia honey, acacia honey and honeydew honey were 0.86, 1.61 and 1.42, respectively. The proposed method provides high separation efficiency and sensitive detection within a short analysis time. Apart from the labeling reaction, it enables the injection of honeys without sample pretreatment. This is the first time that fluorescence detection has been applied for the CE analysis of sugars in honeys. |
| first_indexed | 2024-03-11T05:55:13Z |
| format | Article |
| id | doaj.art-4eef65adebd6486482e2d365a4b87377 |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-11T05:55:13Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-4eef65adebd6486482e2d365a4b873772023-11-17T13:49:43ZengMDPI AGSeparations2297-87392023-02-0110315010.3390/separations10030150Analysis of Sugars in Honey Samples by Capillary Zone Electrophoresis Using Fluorescence DetectionMelinda Andrasi0Gyongyi Gyemant1Zsofi Sajtos2Cynthia Nagy3Department of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem ter 1., H-4032 Debrecen, HungaryDepartment of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem ter 1., H-4032 Debrecen, HungaryDepartment of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem ter 1., H-4032 Debrecen, HungaryDepartment of Inorganic and Analytical Chemistry, University of Debrecen, Egyetem ter 1., H-4032 Debrecen, HungaryThe applicability of capillary electrophoresis (CE) with light-emitting diode-induced fluorescence detection (LEDIF) for the separation of sugars in honey samples was studied. An amount of 25 mM ammonium acetate (pH 4.5) with 0.3% polyethylene oxide (PEO) was found to be optimal for the efficient separation of carbohydrates. 8-aminopyrene-1,3,6-trisulfonic acid (APTS) was used for the labeling of the carbohydrate standards and honey sugars for fluorescence detection. The optimized method was applied in the quantitative analysis of fructose and glucose by direct injection of honey samples. Apart from the labeling reaction, no other sample preparation was performed. The mean values of the fructose/glucose ratio for phacelia honey, acacia honey and honeydew honey were 0.86, 1.61 and 1.42, respectively. The proposed method provides high separation efficiency and sensitive detection within a short analysis time. Apart from the labeling reaction, it enables the injection of honeys without sample pretreatment. This is the first time that fluorescence detection has been applied for the CE analysis of sugars in honeys.https://www.mdpi.com/2297-8739/10/3/150capillary electrophoresisfluorescence detectionhoneysugars |
| spellingShingle | Melinda Andrasi Gyongyi Gyemant Zsofi Sajtos Cynthia Nagy Analysis of Sugars in Honey Samples by Capillary Zone Electrophoresis Using Fluorescence Detection Separations capillary electrophoresis fluorescence detection honey sugars |
| title | Analysis of Sugars in Honey Samples by Capillary Zone Electrophoresis Using Fluorescence Detection |
| title_full | Analysis of Sugars in Honey Samples by Capillary Zone Electrophoresis Using Fluorescence Detection |
| title_fullStr | Analysis of Sugars in Honey Samples by Capillary Zone Electrophoresis Using Fluorescence Detection |
| title_full_unstemmed | Analysis of Sugars in Honey Samples by Capillary Zone Electrophoresis Using Fluorescence Detection |
| title_short | Analysis of Sugars in Honey Samples by Capillary Zone Electrophoresis Using Fluorescence Detection |
| title_sort | analysis of sugars in honey samples by capillary zone electrophoresis using fluorescence detection |
| topic | capillary electrophoresis fluorescence detection honey sugars |
| url | https://www.mdpi.com/2297-8739/10/3/150 |
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