Profile of antibiotic resistance of Staphylococcus aureus isolated from patients and bacterial carriers of the Astrakhan region as the basis for the selection of bacteriophage producing strains for further combined phage therapy
Staphylococcus aureus has a high level of antibiotic resistance and is a common cause of nosocomial infections, which primarily requires development of new therapeutic strategies.Aim of the study was to investigate the level and spectrum of antibiotic resistance of S. aureus isolates for further iso...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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Russian Academy of Sciences, Siberian Branch Publishing House
2024-01-01
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| Series: | Сибирский научный медицинский журнал |
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| Online Access: | https://sibmed.elpub.ru/jour/article/view/1317 |
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| author | R. O. Abdrakhmanova E. G. Tazova G. N. Genatullina G. R. Baeva A. D. Daudova Yu. Z. Demina A. L. Yasenyavskaya O. V. Rubalsky |
| author_facet | R. O. Abdrakhmanova E. G. Tazova G. N. Genatullina G. R. Baeva A. D. Daudova Yu. Z. Demina A. L. Yasenyavskaya O. V. Rubalsky |
| author_sort | R. O. Abdrakhmanova |
| collection | DOAJ |
| description | Staphylococcus aureus has a high level of antibiotic resistance and is a common cause of nosocomial infections, which primarily requires development of new therapeutic strategies.Aim of the study was to investigate the level and spectrum of antibiotic resistance of S. aureus isolates for further isolation of virulent bacteriophages.Material and methods. The study used samples of clinical material obtained on the basis of the City Clinical Hospital No. 3 named after S.M. Kirov (Astrakhan). Bacterial strains isolated from patients were identified on the basis of morphological, cultural, biochemical and molecular genetic characteristics. The determination of the resistance of the isolated cultures was carried out by the disco-diffusion method using standardized commercial discs with antibiotics, in accordance with the guidelines.Results. Evaluation of antibiotic resistance of Staphylococcus aureus isolated during the study of clinical material from patients and analysis of sensitivity level of isolated strains of S. aureus allow us to conclude that all S. aureus isolated from patients are resistant to at least one antibiotic, and most strains are characterized by multidrug resistance to antibiotics.Conclusions. The presence of multi-resistant strains indicates the need to search for new approaches in the treatment of staphylococcal infection and to develop effective means for combination therapy based on virulent staphylococcal bacteriophages. |
| first_indexed | 2024-03-08T14:32:52Z |
| format | Article |
| id | doaj.art-4ef3db452cf94b9db485e273780732bd |
| institution | Directory Open Access Journal |
| issn | 2410-2512 2410-2520 |
| language | Russian |
| last_indexed | 2024-04-25T01:49:43Z |
| publishDate | 2024-01-01 |
| publisher | Russian Academy of Sciences, Siberian Branch Publishing House |
| record_format | Article |
| series | Сибирский научный медицинский журнал |
| spelling | doaj.art-4ef3db452cf94b9db485e273780732bd2024-03-07T18:50:01ZrusRussian Academy of Sciences, Siberian Branch Publishing HouseСибирский научный медицинский журнал2410-25122410-25202024-01-0143612512910.18699/SSMJ20230615567Profile of antibiotic resistance of Staphylococcus aureus isolated from patients and bacterial carriers of the Astrakhan region as the basis for the selection of bacteriophage producing strains for further combined phage therapyR. O. Abdrakhmanova0E. G. Tazova1G. N. Genatullina2G. R. Baeva3A. D. Daudova4Yu. Z. Demina5A. L. Yasenyavskaya6O. V. Rubalsky7Astrakhan State Medical University of Minzdrav of RussiaAstrakhan State Medical University of Minzdrav of RussiaAstrakhan State Medical University of Minzdrav of RussiaAstrakhan State Medical University of Minzdrav of RussiaAstrakhan State Medical University of Minzdrav of RussiaAstrakhan State Medical University of Minzdrav of RussiaAstrakhan State Medical University of Minzdrav of RussiaAstrakhan State Medical University of Minzdrav of RussiaStaphylococcus aureus has a high level of antibiotic resistance and is a common cause of nosocomial infections, which primarily requires development of new therapeutic strategies.Aim of the study was to investigate the level and spectrum of antibiotic resistance of S. aureus isolates for further isolation of virulent bacteriophages.Material and methods. The study used samples of clinical material obtained on the basis of the City Clinical Hospital No. 3 named after S.M. Kirov (Astrakhan). Bacterial strains isolated from patients were identified on the basis of morphological, cultural, biochemical and molecular genetic characteristics. The determination of the resistance of the isolated cultures was carried out by the disco-diffusion method using standardized commercial discs with antibiotics, in accordance with the guidelines.Results. Evaluation of antibiotic resistance of Staphylococcus aureus isolated during the study of clinical material from patients and analysis of sensitivity level of isolated strains of S. aureus allow us to conclude that all S. aureus isolated from patients are resistant to at least one antibiotic, and most strains are characterized by multidrug resistance to antibiotics.Conclusions. The presence of multi-resistant strains indicates the need to search for new approaches in the treatment of staphylococcal infection and to develop effective means for combination therapy based on virulent staphylococcal bacteriophages.https://sibmed.elpub.ru/jour/article/view/1317antibiotic resistancebacteriophageantibioticsmethicillin-resistant staphylococcus aureus |
| spellingShingle | R. O. Abdrakhmanova E. G. Tazova G. N. Genatullina G. R. Baeva A. D. Daudova Yu. Z. Demina A. L. Yasenyavskaya O. V. Rubalsky Profile of antibiotic resistance of Staphylococcus aureus isolated from patients and bacterial carriers of the Astrakhan region as the basis for the selection of bacteriophage producing strains for further combined phage therapy Сибирский научный медицинский журнал antibiotic resistance bacteriophage antibiotics methicillin-resistant staphylococcus aureus |
| title | Profile of antibiotic resistance of Staphylococcus aureus isolated from patients and bacterial carriers of the Astrakhan region as the basis for the selection of bacteriophage producing strains for further combined phage therapy |
| title_full | Profile of antibiotic resistance of Staphylococcus aureus isolated from patients and bacterial carriers of the Astrakhan region as the basis for the selection of bacteriophage producing strains for further combined phage therapy |
| title_fullStr | Profile of antibiotic resistance of Staphylococcus aureus isolated from patients and bacterial carriers of the Astrakhan region as the basis for the selection of bacteriophage producing strains for further combined phage therapy |
| title_full_unstemmed | Profile of antibiotic resistance of Staphylococcus aureus isolated from patients and bacterial carriers of the Astrakhan region as the basis for the selection of bacteriophage producing strains for further combined phage therapy |
| title_short | Profile of antibiotic resistance of Staphylococcus aureus isolated from patients and bacterial carriers of the Astrakhan region as the basis for the selection of bacteriophage producing strains for further combined phage therapy |
| title_sort | profile of antibiotic resistance of staphylococcus aureus isolated from patients and bacterial carriers of the astrakhan region as the basis for the selection of bacteriophage producing strains for further combined phage therapy |
| topic | antibiotic resistance bacteriophage antibiotics methicillin-resistant staphylococcus aureus |
| url | https://sibmed.elpub.ru/jour/article/view/1317 |
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