The inhibitory effects of Xiao-Gao-Jiang-Zhuo-containing serum on adipogenesis in 3T3-L1 preadipocytes
Background. Obesity and related metabolic diseases are becoming a worldwide epidemic, leading to increased mortality and heavy medical costs. Our Chinese herbal formula Xiao-Gao-Jiang-Zhuo (XGJZ) has remarkable effects on curing obese patients in the clinic, but the cellular and molecular basis rema...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Palacký University Olomouc, Faculty of Medicine and Dentistry
2024-03-01
|
| Series: | Biomedical Papers |
| Subjects: | |
| Online Access: | https://biomed.papers.upol.cz/artkey/bio-202401-0003_the-inhibitory-effects-of-xiao-gao-jiang-zhuo-containing-serum-on-adipogenesis-in-3t3-l1-preadipocytes.php |
| _version_ | 1797265175931256832 |
|---|---|
| author | Liang Chen Yu Han Jing Li Chunpeng Feng Chen Chen Ting Ye |
| author_facet | Liang Chen Yu Han Jing Li Chunpeng Feng Chen Chen Ting Ye |
| author_sort | Liang Chen |
| collection | DOAJ |
| description | Background. Obesity and related metabolic diseases are becoming a worldwide epidemic, leading to increased mortality and heavy medical costs. Our Chinese herbal formula Xiao-Gao-Jiang-Zhuo (XGJZ) has remarkable effects on curing obese patients in the clinic, but the cellular and molecular basis remains unknown. This study aimed to reveal the molecular mechanism involved in adipogenesis in vitro. Methods. Chinese herbal formula XGJZ-containing serum was prepared from XGJZ-treated obesity model rats. The function of XGJZ-containing serum was validated in 3T3-L1 preadipocytes. Oil O staining was performed to determine intracellular lipid accumulation in differentiated 3T3-L1 cells. The expression of pro-adipogenic transcription factors was measured to further validate the adipogenesis of 3T3-L1 adipocytes. The contents of triglyceride (TG), free fatty acid (FFA), and glycerin, along with the activities of lipid metabolism-related enzymes (including FAT, FATP1, DGAT, GPAT, ATGL, and HSL) were measured to study the lipogenesis in 3T3-L1 adipocytes. Results. XGJZ-containing serum inhibited 3T3-L1 differentiation, decreased intracellular lipid accumulation, and suppressed the expression of pro-adipogenic transcription factors in differentiated 3T3-L1 cells. The contents of TG, FFA, and glycerin were decreased when treated with XGJZ-containing serum, which also modulated lipid metabolism-related enzyme activities. The activities of fatty acid transporters (FAT, FATP1) and lipid mobilization enzymes (ATGL, HSL) were promoted, while activities of triglyceride biosynthesis enzymes (DGAT, GPAT) were attenuated in differentiated 3T3-L1 cells. Conclusion. XGJZ-containing serum has inhibitory effects on adipogenesis in 3T3-L1 preadipocytes, affirming the effect of XGJZ in treating obesity. It provides evidence for the mechanism of obesity. |
| first_indexed | 2024-04-25T00:40:38Z |
| format | Article |
| id | doaj.art-4ef76b3dd0524209a4c95553e7f16a21 |
| institution | Directory Open Access Journal |
| issn | 1213-8118 1804-7521 |
| language | English |
| last_indexed | 2024-04-25T00:40:38Z |
| publishDate | 2024-03-01 |
| publisher | Palacký University Olomouc, Faculty of Medicine and Dentistry |
| record_format | Article |
| series | Biomedical Papers |
| spelling | doaj.art-4ef76b3dd0524209a4c95553e7f16a212024-03-12T09:46:45ZengPalacký University Olomouc, Faculty of Medicine and DentistryBiomedical Papers1213-81181804-75212024-03-011681253410.5507/bp.2023.016bio-202401-0003The inhibitory effects of Xiao-Gao-Jiang-Zhuo-containing serum on adipogenesis in 3T3-L1 preadipocytesLiang Chen0Yu Han1Jing Li2Chunpeng Feng3Chen Chen4Ting Ye5Department of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, ChinaHospital Administration Office, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaDepartment of Endocrinology, Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University, Urumqi 830000, ChinaDepartment of Endocrinology, Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing 100053, ChinaDepartment of Endocrinology, Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University, Urumqi 830000, ChinaDepartment of Endocrinology, Hospital of Traditional Chinese Medicine Affiliated to Xinjiang Medical University, Urumqi 830000, ChinaBackground. Obesity and related metabolic diseases are becoming a worldwide epidemic, leading to increased mortality and heavy medical costs. Our Chinese herbal formula Xiao-Gao-Jiang-Zhuo (XGJZ) has remarkable effects on curing obese patients in the clinic, but the cellular and molecular basis remains unknown. This study aimed to reveal the molecular mechanism involved in adipogenesis in vitro. Methods. Chinese herbal formula XGJZ-containing serum was prepared from XGJZ-treated obesity model rats. The function of XGJZ-containing serum was validated in 3T3-L1 preadipocytes. Oil O staining was performed to determine intracellular lipid accumulation in differentiated 3T3-L1 cells. The expression of pro-adipogenic transcription factors was measured to further validate the adipogenesis of 3T3-L1 adipocytes. The contents of triglyceride (TG), free fatty acid (FFA), and glycerin, along with the activities of lipid metabolism-related enzymes (including FAT, FATP1, DGAT, GPAT, ATGL, and HSL) were measured to study the lipogenesis in 3T3-L1 adipocytes. Results. XGJZ-containing serum inhibited 3T3-L1 differentiation, decreased intracellular lipid accumulation, and suppressed the expression of pro-adipogenic transcription factors in differentiated 3T3-L1 cells. The contents of TG, FFA, and glycerin were decreased when treated with XGJZ-containing serum, which also modulated lipid metabolism-related enzyme activities. The activities of fatty acid transporters (FAT, FATP1) and lipid mobilization enzymes (ATGL, HSL) were promoted, while activities of triglyceride biosynthesis enzymes (DGAT, GPAT) were attenuated in differentiated 3T3-L1 cells. Conclusion. XGJZ-containing serum has inhibitory effects on adipogenesis in 3T3-L1 preadipocytes, affirming the effect of XGJZ in treating obesity. It provides evidence for the mechanism of obesity.https://biomed.papers.upol.cz/artkey/bio-202401-0003_the-inhibitory-effects-of-xiao-gao-jiang-zhuo-containing-serum-on-adipogenesis-in-3t3-l1-preadipocytes.phpxgjzlipid accumulationadipokines3t3-l1 preadipocytes |
| spellingShingle | Liang Chen Yu Han Jing Li Chunpeng Feng Chen Chen Ting Ye The inhibitory effects of Xiao-Gao-Jiang-Zhuo-containing serum on adipogenesis in 3T3-L1 preadipocytes Biomedical Papers xgjz lipid accumulation adipokines 3t3-l1 preadipocytes |
| title | The inhibitory effects of Xiao-Gao-Jiang-Zhuo-containing serum on adipogenesis in 3T3-L1 preadipocytes |
| title_full | The inhibitory effects of Xiao-Gao-Jiang-Zhuo-containing serum on adipogenesis in 3T3-L1 preadipocytes |
| title_fullStr | The inhibitory effects of Xiao-Gao-Jiang-Zhuo-containing serum on adipogenesis in 3T3-L1 preadipocytes |
| title_full_unstemmed | The inhibitory effects of Xiao-Gao-Jiang-Zhuo-containing serum on adipogenesis in 3T3-L1 preadipocytes |
| title_short | The inhibitory effects of Xiao-Gao-Jiang-Zhuo-containing serum on adipogenesis in 3T3-L1 preadipocytes |
| title_sort | inhibitory effects of xiao gao jiang zhuo containing serum on adipogenesis in 3t3 l1 preadipocytes |
| topic | xgjz lipid accumulation adipokines 3t3-l1 preadipocytes |
| url | https://biomed.papers.upol.cz/artkey/bio-202401-0003_the-inhibitory-effects-of-xiao-gao-jiang-zhuo-containing-serum-on-adipogenesis-in-3t3-l1-preadipocytes.php |
| work_keys_str_mv | AT liangchen theinhibitoryeffectsofxiaogaojiangzhuocontainingserumonadipogenesisin3t3l1preadipocytes AT yuhan theinhibitoryeffectsofxiaogaojiangzhuocontainingserumonadipogenesisin3t3l1preadipocytes AT jingli theinhibitoryeffectsofxiaogaojiangzhuocontainingserumonadipogenesisin3t3l1preadipocytes AT chunpengfeng theinhibitoryeffectsofxiaogaojiangzhuocontainingserumonadipogenesisin3t3l1preadipocytes AT chenchen theinhibitoryeffectsofxiaogaojiangzhuocontainingserumonadipogenesisin3t3l1preadipocytes AT tingye theinhibitoryeffectsofxiaogaojiangzhuocontainingserumonadipogenesisin3t3l1preadipocytes AT liangchen inhibitoryeffectsofxiaogaojiangzhuocontainingserumonadipogenesisin3t3l1preadipocytes AT yuhan inhibitoryeffectsofxiaogaojiangzhuocontainingserumonadipogenesisin3t3l1preadipocytes AT jingli inhibitoryeffectsofxiaogaojiangzhuocontainingserumonadipogenesisin3t3l1preadipocytes AT chunpengfeng inhibitoryeffectsofxiaogaojiangzhuocontainingserumonadipogenesisin3t3l1preadipocytes AT chenchen inhibitoryeffectsofxiaogaojiangzhuocontainingserumonadipogenesisin3t3l1preadipocytes AT tingye inhibitoryeffectsofxiaogaojiangzhuocontainingserumonadipogenesisin3t3l1preadipocytes |