Dose and Time-Dependent Lipopolysaccharide Exposure on A549 Cell Model Influences Pro-Inflammatory Cytokine Interleukin 8
Hyperinflammation in COVID-19 patients is one of the causes of the high mortality rate of COVID-19. An in vitro model mimicking the inflammatory responses in COVID-19 patients is important in the efforts of finding new drug candidates for this disease. Lipopolysaccharide (LPS) can increase the proin...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Universitas Padjajaran
2023-06-01
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| Series: | Majalah Kedokteran Bandung |
| Subjects: | |
| Online Access: | https://journal.fk.unpad.ac.id/index.php/mkb/article/view/3143 |
| _version_ | 1797263117546160128 |
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| author | Sarah Sumayyah Prayoga Suryadarma Rachmawati Noverina Wireni Ayuningtyas Firman Fuad Wirakusumah Ahmad Faried |
| author_facet | Sarah Sumayyah Prayoga Suryadarma Rachmawati Noverina Wireni Ayuningtyas Firman Fuad Wirakusumah Ahmad Faried |
| author_sort | Sarah Sumayyah |
| collection | DOAJ |
| description | Hyperinflammation in COVID-19 patients is one of the causes of the high mortality rate of COVID-19. An in vitro model mimicking the inflammatory responses in COVID-19 patients is important in the efforts of finding new drug candidates for this disease. Lipopolysaccharide (LPS) can increase the proinflammatory cytokine interleukin 8 in response to the presence of foreign substances. This preliminary study sought to explore the use of the A549 cells as an in vitro inflammatory model. This study was conducted from August to November 2022 at the stem cell research and development laboratory of Bio Farma Indonesia. The exposure of 100, 500, and 1000 g/mL doses of LPS administered for 24, 72, and 120 hours on the A549 cells was analyzed for cell viability, population doubling time (PDT), and the presence of proinflammatory cytokine IL-8. The group differences were examined using one- and two-way analysis of variance in IBM SPSS Statistics Version 29, with a p-value of 0.05 considered significant. Cells exposed to a dose of 1000 g/mL LPS had a lower viability and a higher proliferation rate (p<0.05) based on the viability and PDT. Viability, PDT, and pro-inflammatory cytokines showed concentration- and time-dependent responses. Therefore, increased levels of the proinflammatory cytokine IL-8 in cells exposed to LPS at a dose of 1000 g/mL for 24 hours can be used as a mimic to study hyperinflammation in COVID-19 patients. |
| first_indexed | 2024-04-25T00:07:55Z |
| format | Article |
| id | doaj.art-4ef8443c771e45c2ac87adac7915fcbd |
| institution | Directory Open Access Journal |
| issn | 0126-074X 2338-6223 |
| language | English |
| last_indexed | 2024-04-25T00:07:55Z |
| publishDate | 2023-06-01 |
| publisher | Universitas Padjajaran |
| record_format | Article |
| series | Majalah Kedokteran Bandung |
| spelling | doaj.art-4ef8443c771e45c2ac87adac7915fcbd2024-03-14T03:39:29ZengUniversitas PadjajaranMajalah Kedokteran Bandung0126-074X2338-62232023-06-01552859110.15395/mkb.v55n2.31431572Dose and Time-Dependent Lipopolysaccharide Exposure on A549 Cell Model Influences Pro-Inflammatory Cytokine Interleukin 8Sarah Sumayyah0Prayoga Suryadarma1Rachmawati Noverina2Wireni Ayuningtyas3Firman Fuad Wirakusumah4Ahmad Faried5Biotechnology Study Program, Postgraduate School, Bogor Agricultural Institute, BogorDepartment of Agricultural Industrial Technology, Faculty of Agricultural Technology, Bogor Agricultural Institute, BogorStem Cell Research and Development, Bio Farma, BandungFaculty of Medicine, Universitas Padjadjaran, BandungDepartment of Obstetrics and Gynecology, Faculty of Medicine Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital BandungDepartment of Neurosurgery, Faculty of Medicine Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital BandungHyperinflammation in COVID-19 patients is one of the causes of the high mortality rate of COVID-19. An in vitro model mimicking the inflammatory responses in COVID-19 patients is important in the efforts of finding new drug candidates for this disease. Lipopolysaccharide (LPS) can increase the proinflammatory cytokine interleukin 8 in response to the presence of foreign substances. This preliminary study sought to explore the use of the A549 cells as an in vitro inflammatory model. This study was conducted from August to November 2022 at the stem cell research and development laboratory of Bio Farma Indonesia. The exposure of 100, 500, and 1000 g/mL doses of LPS administered for 24, 72, and 120 hours on the A549 cells was analyzed for cell viability, population doubling time (PDT), and the presence of proinflammatory cytokine IL-8. The group differences were examined using one- and two-way analysis of variance in IBM SPSS Statistics Version 29, with a p-value of 0.05 considered significant. Cells exposed to a dose of 1000 g/mL LPS had a lower viability and a higher proliferation rate (p<0.05) based on the viability and PDT. Viability, PDT, and pro-inflammatory cytokines showed concentration- and time-dependent responses. Therefore, increased levels of the proinflammatory cytokine IL-8 in cells exposed to LPS at a dose of 1000 g/mL for 24 hours can be used as a mimic to study hyperinflammation in COVID-19 patients.https://journal.fk.unpad.ac.id/index.php/mkb/article/view/3143a549 cell, inflammation, interleukin 8, lipopolysaccharide exposure |
| spellingShingle | Sarah Sumayyah Prayoga Suryadarma Rachmawati Noverina Wireni Ayuningtyas Firman Fuad Wirakusumah Ahmad Faried Dose and Time-Dependent Lipopolysaccharide Exposure on A549 Cell Model Influences Pro-Inflammatory Cytokine Interleukin 8 Majalah Kedokteran Bandung a549 cell, inflammation, interleukin 8, lipopolysaccharide exposure |
| title | Dose and Time-Dependent Lipopolysaccharide Exposure on A549 Cell Model Influences Pro-Inflammatory Cytokine Interleukin 8 |
| title_full | Dose and Time-Dependent Lipopolysaccharide Exposure on A549 Cell Model Influences Pro-Inflammatory Cytokine Interleukin 8 |
| title_fullStr | Dose and Time-Dependent Lipopolysaccharide Exposure on A549 Cell Model Influences Pro-Inflammatory Cytokine Interleukin 8 |
| title_full_unstemmed | Dose and Time-Dependent Lipopolysaccharide Exposure on A549 Cell Model Influences Pro-Inflammatory Cytokine Interleukin 8 |
| title_short | Dose and Time-Dependent Lipopolysaccharide Exposure on A549 Cell Model Influences Pro-Inflammatory Cytokine Interleukin 8 |
| title_sort | dose and time dependent lipopolysaccharide exposure on a549 cell model influences pro inflammatory cytokine interleukin 8 |
| topic | a549 cell, inflammation, interleukin 8, lipopolysaccharide exposure |
| url | https://journal.fk.unpad.ac.id/index.php/mkb/article/view/3143 |
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