Acute response of circulating vascular regulating microRNAs during and after high-intensity and high-volume cycling in children
Aim: The aim of the present study was to analyze the response of vascular circulating microRNAs (miRNAs; miR-16, miR-21, miR-126) and the VEGF mRNA following an acute bout of HIIT and HVT in children. Methods: Twelve healthy competitive young male cyclists (14.4 ± 0.8 yrs; 57.9 ± 9.4 ml·min-1·kg-1 p...
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| Format: | Article |
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Frontiers Media S.A.
2016-03-01
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| Series: | Frontiers in Physiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fphys.2016.00092/full |
| _version_ | 1818058133453406208 |
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| author | Yvonne eKilian Yvonne eKilian Udo F Wehmeier Patrick eWahl Patrick eWahl Patrick eWahl Joachim eMester Joachim eMester Thomas eHilberg Billy eSperlich |
| author_facet | Yvonne eKilian Yvonne eKilian Udo F Wehmeier Patrick eWahl Patrick eWahl Patrick eWahl Joachim eMester Joachim eMester Thomas eHilberg Billy eSperlich |
| author_sort | Yvonne eKilian |
| collection | DOAJ |
| description | Aim: The aim of the present study was to analyze the response of vascular circulating microRNAs (miRNAs; miR-16, miR-21, miR-126) and the VEGF mRNA following an acute bout of HIIT and HVT in children. Methods: Twelve healthy competitive young male cyclists (14.4 ± 0.8 yrs; 57.9 ± 9.4 ml·min-1·kg-1 peak oxygen uptake) performed one session of high intensity 4x4 min intervals (HIIT) at 90-95% peak power output, each interval separated by 3 min of active recovery, and one high volume session (HVT) consisting of a constant load exercise for 90 min at 60% peak power output. Capillary blood from the earlobe was collected under resting conditions, during exercise (d1 = 20 min, d2 = 30 min, d3 = 60 min), and 0, 30, 60, 180 min after the exercise to determine miR-16, -21, -126 and VEGF mRNA.Results: HVT significantly increased miR-16 and miR-126 during and after the exercise compared to pre values, whereas HIIT showed no significant influence on the miRNAs compared to pre values. VEGF mRNA significantly increased during and after HIIT (d1, 30`, 60`, 180`) and HVT (d3, 0`, 60`). Conclusion: Results of the present investigation suggest a volume dependent exercise regulation of vascular regulating miRNAs (miR-16, miR-21, miR-126) in children. In line with previous data, our data show that acute exercise can alter circulating miRNAs profiles that might be used as novel biomarkers to monitor acute and chronic changes due to exercise in various tissues. |
| first_indexed | 2024-12-10T12:55:47Z |
| format | Article |
| id | doaj.art-4ef8c0bb1ffc414ab48a9c43ae7a9d7c |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-10T12:55:47Z |
| publishDate | 2016-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-4ef8c0bb1ffc414ab48a9c43ae7a9d7c2022-12-22T01:48:06ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2016-03-01710.3389/fphys.2016.00092165099Acute response of circulating vascular regulating microRNAs during and after high-intensity and high-volume cycling in childrenYvonne eKilian0Yvonne eKilian1Udo F Wehmeier2Patrick eWahl3Patrick eWahl4Patrick eWahl5Joachim eMester6Joachim eMester7Thomas eHilberg8Billy eSperlich9German Sport University CologneGerman Sport University CologneUniversity of WuppertalGerman Sport University CologneGerman Sport University CologneGerman Sport University CologneGerman Sport University CologneGerman Sport University CologneUniversity of WuppertalUniversity of WürzburgAim: The aim of the present study was to analyze the response of vascular circulating microRNAs (miRNAs; miR-16, miR-21, miR-126) and the VEGF mRNA following an acute bout of HIIT and HVT in children. Methods: Twelve healthy competitive young male cyclists (14.4 ± 0.8 yrs; 57.9 ± 9.4 ml·min-1·kg-1 peak oxygen uptake) performed one session of high intensity 4x4 min intervals (HIIT) at 90-95% peak power output, each interval separated by 3 min of active recovery, and one high volume session (HVT) consisting of a constant load exercise for 90 min at 60% peak power output. Capillary blood from the earlobe was collected under resting conditions, during exercise (d1 = 20 min, d2 = 30 min, d3 = 60 min), and 0, 30, 60, 180 min after the exercise to determine miR-16, -21, -126 and VEGF mRNA.Results: HVT significantly increased miR-16 and miR-126 during and after the exercise compared to pre values, whereas HIIT showed no significant influence on the miRNAs compared to pre values. VEGF mRNA significantly increased during and after HIIT (d1, 30`, 60`, 180`) and HVT (d3, 0`, 60`). Conclusion: Results of the present investigation suggest a volume dependent exercise regulation of vascular regulating miRNAs (miR-16, miR-21, miR-126) in children. In line with previous data, our data show that acute exercise can alter circulating miRNAs profiles that might be used as novel biomarkers to monitor acute and chronic changes due to exercise in various tissues.http://journal.frontiersin.org/Journal/10.3389/fphys.2016.00092/fullExerciseMicroRNAsChildrenEndurancetraining adaptation |
| spellingShingle | Yvonne eKilian Yvonne eKilian Udo F Wehmeier Patrick eWahl Patrick eWahl Patrick eWahl Joachim eMester Joachim eMester Thomas eHilberg Billy eSperlich Acute response of circulating vascular regulating microRNAs during and after high-intensity and high-volume cycling in children Frontiers in Physiology Exercise MicroRNAs Children Endurance training adaptation |
| title | Acute response of circulating vascular regulating microRNAs during and after high-intensity and high-volume cycling in children |
| title_full | Acute response of circulating vascular regulating microRNAs during and after high-intensity and high-volume cycling in children |
| title_fullStr | Acute response of circulating vascular regulating microRNAs during and after high-intensity and high-volume cycling in children |
| title_full_unstemmed | Acute response of circulating vascular regulating microRNAs during and after high-intensity and high-volume cycling in children |
| title_short | Acute response of circulating vascular regulating microRNAs during and after high-intensity and high-volume cycling in children |
| title_sort | acute response of circulating vascular regulating micrornas during and after high intensity and high volume cycling in children |
| topic | Exercise MicroRNAs Children Endurance training adaptation |
| url | http://journal.frontiersin.org/Journal/10.3389/fphys.2016.00092/full |
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