Heartwood of <i>Dalbergia cochinchinensis</i>: 4,7,2′-Trihydroxy-4′-methoxyisoflavanol and 6,4′-Dihydroxy-7-methoxyflavane Reduce Cytokine and Chemokine Expression In Vitro
<i>Dalbergia cochinchinensis</i> has been widely used in traditional medicine because of its flavonoids; however, the impact of the flavonoids to modulate the inflammatory response to oral cells remains to be described. For this aim, we isolated 4,7,2′-trihydroxy-4′-methoxyisoflavanol (4...
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MDPI AG
2022-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/4/1321 |
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| author | Feng Shao Layla Panahipour Mariane Beatriz Sordi Fangrui Tang Ronghua Liu Reinhard Gruber |
| author_facet | Feng Shao Layla Panahipour Mariane Beatriz Sordi Fangrui Tang Ronghua Liu Reinhard Gruber |
| author_sort | Feng Shao |
| collection | DOAJ |
| description | <i>Dalbergia cochinchinensis</i> has been widely used in traditional medicine because of its flavonoids; however, the impact of the flavonoids to modulate the inflammatory response to oral cells remains to be described. For this aim, we isolated 4,7,2′-trihydroxy-4′-methoxyisoflavanol (472T4MIF) and 6,4′-dihydroxy-7-methoxyflavane (64D7MF) from the heartwood of <i>D. cochinchinensis</i> and confirmed the chemical structure by nuclear magnetic resonance. We show here that both flavonoids are inhibitors of an inflammatory response of murine RAW 264.7 inflammatory macrophages stimulated by LPS. This is indicated by interleukin (IL)1, IL6, and chemokine CCL2 production besides the phosphorylation of p65. Consistently, in primary murine macrophages, both flavonoids decreased the inflammatory response by lowering LPS-induced IL1 and IL6 expression. To introduce oral cells, we have used human gingival fibroblasts and provoked the inflammatory response by exposing them to IL1β and TNFα. Under these conditions, 472T4MIF, but not 64D7MF, reduced the expression of chemokines CXCL1 and CXCL2. Taken together, we identified two flavonoids that can reduce the expression of cytokines and chemokines in macrophages and fibroblastic cells. |
| first_indexed | 2024-03-09T21:20:42Z |
| format | Article |
| id | doaj.art-4efbd28174c34a9bb1fa49fe89f8c82b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:20:42Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4efbd28174c34a9bb1fa49fe89f8c82b2023-11-23T21:22:10ZengMDPI AGMolecules1420-30492022-02-01274132110.3390/molecules27041321Heartwood of <i>Dalbergia cochinchinensis</i>: 4,7,2′-Trihydroxy-4′-methoxyisoflavanol and 6,4′-Dihydroxy-7-methoxyflavane Reduce Cytokine and Chemokine Expression In VitroFeng Shao0Layla Panahipour1Mariane Beatriz Sordi2Fangrui Tang3Ronghua Liu4Reinhard Gruber5Department of Oral Biology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Oral Biology, Medical University of Vienna, 1090 Vienna, AustriaDepartment of Oral Biology, Medical University of Vienna, 1090 Vienna, AustriaKey Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang 330004, ChinaKey Laboratory of Modern Preparation of Traditional Chinese Medicine, Ministry of Education, Jiangxi University of Chinese Medicine, Nanchang 330004, ChinaDepartment of Oral Biology, Medical University of Vienna, 1090 Vienna, Austria<i>Dalbergia cochinchinensis</i> has been widely used in traditional medicine because of its flavonoids; however, the impact of the flavonoids to modulate the inflammatory response to oral cells remains to be described. For this aim, we isolated 4,7,2′-trihydroxy-4′-methoxyisoflavanol (472T4MIF) and 6,4′-dihydroxy-7-methoxyflavane (64D7MF) from the heartwood of <i>D. cochinchinensis</i> and confirmed the chemical structure by nuclear magnetic resonance. We show here that both flavonoids are inhibitors of an inflammatory response of murine RAW 264.7 inflammatory macrophages stimulated by LPS. This is indicated by interleukin (IL)1, IL6, and chemokine CCL2 production besides the phosphorylation of p65. Consistently, in primary murine macrophages, both flavonoids decreased the inflammatory response by lowering LPS-induced IL1 and IL6 expression. To introduce oral cells, we have used human gingival fibroblasts and provoked the inflammatory response by exposing them to IL1β and TNFα. Under these conditions, 472T4MIF, but not 64D7MF, reduced the expression of chemokines CXCL1 and CXCL2. Taken together, we identified two flavonoids that can reduce the expression of cytokines and chemokines in macrophages and fibroblastic cells.https://www.mdpi.com/1420-3049/27/4/1321flavonoids<i>Dalbergia cochinchinensis</i>macrophagesfibroblasts |
| spellingShingle | Feng Shao Layla Panahipour Mariane Beatriz Sordi Fangrui Tang Ronghua Liu Reinhard Gruber Heartwood of <i>Dalbergia cochinchinensis</i>: 4,7,2′-Trihydroxy-4′-methoxyisoflavanol and 6,4′-Dihydroxy-7-methoxyflavane Reduce Cytokine and Chemokine Expression In Vitro Molecules flavonoids <i>Dalbergia cochinchinensis</i> macrophages fibroblasts |
| title | Heartwood of <i>Dalbergia cochinchinensis</i>: 4,7,2′-Trihydroxy-4′-methoxyisoflavanol and 6,4′-Dihydroxy-7-methoxyflavane Reduce Cytokine and Chemokine Expression In Vitro |
| title_full | Heartwood of <i>Dalbergia cochinchinensis</i>: 4,7,2′-Trihydroxy-4′-methoxyisoflavanol and 6,4′-Dihydroxy-7-methoxyflavane Reduce Cytokine and Chemokine Expression In Vitro |
| title_fullStr | Heartwood of <i>Dalbergia cochinchinensis</i>: 4,7,2′-Trihydroxy-4′-methoxyisoflavanol and 6,4′-Dihydroxy-7-methoxyflavane Reduce Cytokine and Chemokine Expression In Vitro |
| title_full_unstemmed | Heartwood of <i>Dalbergia cochinchinensis</i>: 4,7,2′-Trihydroxy-4′-methoxyisoflavanol and 6,4′-Dihydroxy-7-methoxyflavane Reduce Cytokine and Chemokine Expression In Vitro |
| title_short | Heartwood of <i>Dalbergia cochinchinensis</i>: 4,7,2′-Trihydroxy-4′-methoxyisoflavanol and 6,4′-Dihydroxy-7-methoxyflavane Reduce Cytokine and Chemokine Expression In Vitro |
| title_sort | heartwood of i dalbergia cochinchinensis i 4 7 2 trihydroxy 4 methoxyisoflavanol and 6 4 dihydroxy 7 methoxyflavane reduce cytokine and chemokine expression in vitro |
| topic | flavonoids <i>Dalbergia cochinchinensis</i> macrophages fibroblasts |
| url | https://www.mdpi.com/1420-3049/27/4/1321 |
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