Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria.
BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms, particularly clinically important pathogens. METHODOLOGY/PRINCIPAL FINDINGS: We compared the identification efficiency of MALDI-TOF MS w...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3026826?pdf=render |
| _version_ | 1818020142748008448 |
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| author | Cinzia Benagli Viviana Rossi Marisa Dolina Mauro Tonolla Orlando Petrini |
| author_facet | Cinzia Benagli Viviana Rossi Marisa Dolina Mauro Tonolla Orlando Petrini |
| author_sort | Cinzia Benagli |
| collection | DOAJ |
| description | BACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms, particularly clinically important pathogens. METHODOLOGY/PRINCIPAL FINDINGS: We compared the identification efficiency of MALDI-TOF MS with that of Phoenix®, API® and 16S ribosomal DNA sequence analysis on 1,019 strains obtained from routine diagnostics. Further, we determined the agreement of MALDI-TOF MS identifications as compared to 16S gene sequencing for additional 545 strains belonging to species of Enterococcus, Gardnerella, Staphylococcus, and Streptococcus. For 94.7% of the isolates MALDI-TOF MS results were identical with those obtained with conventional systems. 16S sequencing confirmed MALDI-TOF MS identification in 63% of the discordant results. Agreement of identification of Gardnerella, Enterococcus, Streptococcus and Staphylococcus species between MALDI-TOF MS and traditional method was high (Crohn's kappa values: 0.9 to 0.93). CONCLUSIONS/SIGNIFICANCE: MALDI-TOF MS represents a rapid, reliable and cost-effective identification technique for clinically relevant bacteria. |
| first_indexed | 2024-04-14T08:01:18Z |
| format | Article |
| id | doaj.art-4f00efc452ae4919a65de469b112ed87 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T08:01:18Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4f00efc452ae4919a65de469b112ed872022-12-22T02:04:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0161e1642410.1371/journal.pone.0016424Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria.Cinzia BenagliViviana RossiMarisa DolinaMauro TonollaOrlando PetriniBACKGROUND: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms, particularly clinically important pathogens. METHODOLOGY/PRINCIPAL FINDINGS: We compared the identification efficiency of MALDI-TOF MS with that of Phoenix®, API® and 16S ribosomal DNA sequence analysis on 1,019 strains obtained from routine diagnostics. Further, we determined the agreement of MALDI-TOF MS identifications as compared to 16S gene sequencing for additional 545 strains belonging to species of Enterococcus, Gardnerella, Staphylococcus, and Streptococcus. For 94.7% of the isolates MALDI-TOF MS results were identical with those obtained with conventional systems. 16S sequencing confirmed MALDI-TOF MS identification in 63% of the discordant results. Agreement of identification of Gardnerella, Enterococcus, Streptococcus and Staphylococcus species between MALDI-TOF MS and traditional method was high (Crohn's kappa values: 0.9 to 0.93). CONCLUSIONS/SIGNIFICANCE: MALDI-TOF MS represents a rapid, reliable and cost-effective identification technique for clinically relevant bacteria.http://europepmc.org/articles/PMC3026826?pdf=render |
| spellingShingle | Cinzia Benagli Viviana Rossi Marisa Dolina Mauro Tonolla Orlando Petrini Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria. PLoS ONE |
| title | Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria. |
| title_full | Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria. |
| title_fullStr | Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria. |
| title_full_unstemmed | Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria. |
| title_short | Matrix-assisted laser desorption ionization-time of flight mass spectrometry for the identification of clinically relevant bacteria. |
| title_sort | matrix assisted laser desorption ionization time of flight mass spectrometry for the identification of clinically relevant bacteria |
| url | http://europepmc.org/articles/PMC3026826?pdf=render |
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