A new Desmodesmus sp. from the Tibetan Yamdrok Lake.

Revegetation of exposed sub-soil, while a desirable recovery strategy, often fails due to extreme soil chemical properties, such as low organic matter and pH levels. Microalgae play a key role in maintaining water quality in the lakes and rivers on the Qinghai-Tibet plateau. Plateau microalgae have...

Full description

Bibliographic Details
Main Authors: Jinhu Wang, Qiangying Zhang, Naijiang Chen, Junyu Chen, Jinna Zhou, Jing Li, Yanli Wei, Duo Bu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2022-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0275799
_version_ 1828143077812862976
author Jinhu Wang
Qiangying Zhang
Naijiang Chen
Junyu Chen
Jinna Zhou
Jing Li
Yanli Wei
Duo Bu
author_facet Jinhu Wang
Qiangying Zhang
Naijiang Chen
Junyu Chen
Jinna Zhou
Jing Li
Yanli Wei
Duo Bu
author_sort Jinhu Wang
collection DOAJ
description Revegetation of exposed sub-soil, while a desirable recovery strategy, often fails due to extreme soil chemical properties, such as low organic matter and pH levels. Microalgae play a key role in maintaining water quality in the lakes and rivers on the Qinghai-Tibet plateau. Plateau microalgae have extensive application prospects in environmental purification, biotechnology, medicine and cosmetics, food industry, and renewable energy. To identify the high biomass of microalgae present in nature, microalgae with the greatest biomass were screened from natural water samples through filtration, pre-culture, and plate scribing separation. Following identification via 18S rRNA sequencing as for the Desmodesmus sp., we constructed a neighbor-joining phylogenetic tree. The novel Desmodesmus sp. from the Tibetan Yamdrok Lake were identified through polyphasic taxonomy. Simultaneously, the sequence of the experimental samples and the target species were shown different following the identification and analysis of SNP and InDel loci. The light-absorbing properties of plateau Desmodesmus sp. have been investigated previously. The characteristic absorption peak of Desmodesmus sp. on the plateau was measured at 689 nm in the visible spectrum using full wavelength scanning with a UV-Vis spectrophotometer. For Desmodesmus sp. which is prone to settling in the process of amplification culture. By monitoring the change trend of total nitrogen, total phosphorus, pH and electrical conductivity in algae solution system, we determined that the logarithmic growth phase and the best transfer window of Desmodesmus sp. were at 15-20 days. This study can provide basic research methods for the study of microalgae in high altitude areas, and lay a foundation for the later study and application of microalgae.
first_indexed 2024-04-11T19:52:32Z
format Article
id doaj.art-4f03d757d89b4b46bfadf37fc6e1b41a
institution Directory Open Access Journal
issn 1932-6203
language English
last_indexed 2024-04-11T19:52:32Z
publishDate 2022-01-01
publisher Public Library of Science (PLoS)
record_format Article
series PLoS ONE
spelling doaj.art-4f03d757d89b4b46bfadf37fc6e1b41a2022-12-22T04:06:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011710e027579910.1371/journal.pone.0275799A new Desmodesmus sp. from the Tibetan Yamdrok Lake.Jinhu WangQiangying ZhangNaijiang ChenJunyu ChenJinna ZhouJing LiYanli WeiDuo BuRevegetation of exposed sub-soil, while a desirable recovery strategy, often fails due to extreme soil chemical properties, such as low organic matter and pH levels. Microalgae play a key role in maintaining water quality in the lakes and rivers on the Qinghai-Tibet plateau. Plateau microalgae have extensive application prospects in environmental purification, biotechnology, medicine and cosmetics, food industry, and renewable energy. To identify the high biomass of microalgae present in nature, microalgae with the greatest biomass were screened from natural water samples through filtration, pre-culture, and plate scribing separation. Following identification via 18S rRNA sequencing as for the Desmodesmus sp., we constructed a neighbor-joining phylogenetic tree. The novel Desmodesmus sp. from the Tibetan Yamdrok Lake were identified through polyphasic taxonomy. Simultaneously, the sequence of the experimental samples and the target species were shown different following the identification and analysis of SNP and InDel loci. The light-absorbing properties of plateau Desmodesmus sp. have been investigated previously. The characteristic absorption peak of Desmodesmus sp. on the plateau was measured at 689 nm in the visible spectrum using full wavelength scanning with a UV-Vis spectrophotometer. For Desmodesmus sp. which is prone to settling in the process of amplification culture. By monitoring the change trend of total nitrogen, total phosphorus, pH and electrical conductivity in algae solution system, we determined that the logarithmic growth phase and the best transfer window of Desmodesmus sp. were at 15-20 days. This study can provide basic research methods for the study of microalgae in high altitude areas, and lay a foundation for the later study and application of microalgae.https://doi.org/10.1371/journal.pone.0275799
spellingShingle Jinhu Wang
Qiangying Zhang
Naijiang Chen
Junyu Chen
Jinna Zhou
Jing Li
Yanli Wei
Duo Bu
A new Desmodesmus sp. from the Tibetan Yamdrok Lake.
PLoS ONE
title A new Desmodesmus sp. from the Tibetan Yamdrok Lake.
title_full A new Desmodesmus sp. from the Tibetan Yamdrok Lake.
title_fullStr A new Desmodesmus sp. from the Tibetan Yamdrok Lake.
title_full_unstemmed A new Desmodesmus sp. from the Tibetan Yamdrok Lake.
title_short A new Desmodesmus sp. from the Tibetan Yamdrok Lake.
title_sort new desmodesmus sp from the tibetan yamdrok lake
url https://doi.org/10.1371/journal.pone.0275799
work_keys_str_mv AT jinhuwang anewdesmodesmusspfromthetibetanyamdroklake
AT qiangyingzhang anewdesmodesmusspfromthetibetanyamdroklake
AT naijiangchen anewdesmodesmusspfromthetibetanyamdroklake
AT junyuchen anewdesmodesmusspfromthetibetanyamdroklake
AT jinnazhou anewdesmodesmusspfromthetibetanyamdroklake
AT jingli anewdesmodesmusspfromthetibetanyamdroklake
AT yanliwei anewdesmodesmusspfromthetibetanyamdroklake
AT duobu anewdesmodesmusspfromthetibetanyamdroklake
AT jinhuwang newdesmodesmusspfromthetibetanyamdroklake
AT qiangyingzhang newdesmodesmusspfromthetibetanyamdroklake
AT naijiangchen newdesmodesmusspfromthetibetanyamdroklake
AT junyuchen newdesmodesmusspfromthetibetanyamdroklake
AT jinnazhou newdesmodesmusspfromthetibetanyamdroklake
AT jingli newdesmodesmusspfromthetibetanyamdroklake
AT yanliwei newdesmodesmusspfromthetibetanyamdroklake
AT duobu newdesmodesmusspfromthetibetanyamdroklake