Performance Comparison of Flow-Through Optofluidic Biosensor Designs
Optofluidic flow-through biosensors are being developed for single particle detection, particularly as a tool for pathogen diagnosis. The sensitivity of the biosensor chip depends on design parameters, illumination format (side vs. top), and flow configuration (parabolic, two- and three-dimensional...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Biosensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-6374/11/7/226 |
| _version_ | 1797527523792257024 |
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| author | Joel G. Wright Md Nafiz Amin Holger Schmidt Aaron R. Hawkins |
| author_facet | Joel G. Wright Md Nafiz Amin Holger Schmidt Aaron R. Hawkins |
| author_sort | Joel G. Wright |
| collection | DOAJ |
| description | Optofluidic flow-through biosensors are being developed for single particle detection, particularly as a tool for pathogen diagnosis. The sensitivity of the biosensor chip depends on design parameters, illumination format (side vs. top), and flow configuration (parabolic, two- and three-dimensional hydrodynamic focused (2DHF and 3DHF)). We study the signal differences between various combinations of these design aspects. Our model is validated against a sample of physical devices. We find that side-illumination with 3DHF produces the strongest and consistent signal, but parabolic flow devices process a sample volume more quickly. Practical matters of optical alignment are also discussed, which may affect design choice. |
| first_indexed | 2024-03-10T09:44:55Z |
| format | Article |
| id | doaj.art-4f077ae6024f4414a59f5454d276223d |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T09:44:55Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-4f077ae6024f4414a59f5454d276223d2023-11-22T03:19:51ZengMDPI AGBiosensors2079-63742021-07-0111722610.3390/bios11070226Performance Comparison of Flow-Through Optofluidic Biosensor DesignsJoel G. Wright0Md Nafiz Amin1Holger Schmidt2Aaron R. Hawkins3Electrical and Computer Engineering, Brigham Young University, 450 Engineering Building, Provo, UT 84602, USAElectrical and Computer Engineering, University of California, 1156 High Street, Santa Cruz, CA 95064, USAElectrical and Computer Engineering, University of California, 1156 High Street, Santa Cruz, CA 95064, USAElectrical and Computer Engineering, Brigham Young University, 450 Engineering Building, Provo, UT 84602, USAOptofluidic flow-through biosensors are being developed for single particle detection, particularly as a tool for pathogen diagnosis. The sensitivity of the biosensor chip depends on design parameters, illumination format (side vs. top), and flow configuration (parabolic, two- and three-dimensional hydrodynamic focused (2DHF and 3DHF)). We study the signal differences between various combinations of these design aspects. Our model is validated against a sample of physical devices. We find that side-illumination with 3DHF produces the strongest and consistent signal, but parabolic flow devices process a sample volume more quickly. Practical matters of optical alignment are also discussed, which may affect design choice.https://www.mdpi.com/2079-6374/11/7/226optofluidichydrodynamic focusinglab-on-a-chipbiosensorliquid-core waveguidefluorescence |
| spellingShingle | Joel G. Wright Md Nafiz Amin Holger Schmidt Aaron R. Hawkins Performance Comparison of Flow-Through Optofluidic Biosensor Designs Biosensors optofluidic hydrodynamic focusing lab-on-a-chip biosensor liquid-core waveguide fluorescence |
| title | Performance Comparison of Flow-Through Optofluidic Biosensor Designs |
| title_full | Performance Comparison of Flow-Through Optofluidic Biosensor Designs |
| title_fullStr | Performance Comparison of Flow-Through Optofluidic Biosensor Designs |
| title_full_unstemmed | Performance Comparison of Flow-Through Optofluidic Biosensor Designs |
| title_short | Performance Comparison of Flow-Through Optofluidic Biosensor Designs |
| title_sort | performance comparison of flow through optofluidic biosensor designs |
| topic | optofluidic hydrodynamic focusing lab-on-a-chip biosensor liquid-core waveguide fluorescence |
| url | https://www.mdpi.com/2079-6374/11/7/226 |
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