Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection
The sequential pathology of a genotype XIII Bangladeshi strain of Newcastle disease virus (NDV) was studied in 5-weeks old chickens. Layer chickens of ISA Brown breed were inoculated through the intranasal and intraocular routes with the BD-C161/2010 strain of NDV and examined at different times pos...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
MDPI AG
2020-07-01
|
Series: | Pathogens |
Subjects: | |
Online Access: | https://www.mdpi.com/2076-0817/9/7/539 |
_version_ | 1797563218968707072 |
---|---|
author | Congriev Kumar Kabiraj Tanjin Tamanna Mumu Emdadul Haque Chowdhury Mohammad Rafiqul Islam Mohammed Nooruzzaman |
author_facet | Congriev Kumar Kabiraj Tanjin Tamanna Mumu Emdadul Haque Chowdhury Mohammad Rafiqul Islam Mohammed Nooruzzaman |
author_sort | Congriev Kumar Kabiraj |
collection | DOAJ |
description | The sequential pathology of a genotype XIII Bangladeshi strain of Newcastle disease virus (NDV) was studied in 5-weeks old chickens. Layer chickens of ISA Brown breed were inoculated through the intranasal and intraocular routes with the BD-C161/2010 strain of NDV and examined at different times post-infection (pi). NDV-infected chickens showed depression at 3 days pi (dpi) followed by dropped wings, paralysis and death starting at 4 dpi. Lungs of infected chickens showed hemorrhagic lesions starting at 24 hours pi (hpi) that was followed by pallor and slight contraction by 2 to 3 dpi and subsequently developed into severe hemorrhagic pneumonia with mononuclear cell infiltration. Hemorrhagic and necrotizing lesions were found in different visceral organs including proventriculus, intestine, gut-associated lymphoid tissues, liver and kidneys starting at 3 dpi that progressed rapidly. Severe lymphoid depletion was observed in the thymus, spleen and bursa of Fabricius starting at 1–3 dpi followed by hemorrhages, necrosis, inflammation and atrophy at 4–5 dpi. In the brain, mild neuronal lesions such as focal to diffuse encephalitis with encephalomalacia was observed at 2–3 dpi and moderate and diffuse meningoencephalitis with encephalomalacia at advanced stages. In conclusion, the BD-C161/2010 strain of NDV produced lesions typical of velogenic viscerotropic pathotype of NDV. |
first_indexed | 2024-03-10T18:39:24Z |
format | Article |
id | doaj.art-4f07bcab0c6943719a78fd961e6aaedc |
institution | Directory Open Access Journal |
issn | 2076-0817 |
language | English |
last_indexed | 2024-03-10T18:39:24Z |
publishDate | 2020-07-01 |
publisher | MDPI AG |
record_format | Article |
series | Pathogens |
spelling | doaj.art-4f07bcab0c6943719a78fd961e6aaedc2023-11-20T05:58:05ZengMDPI AGPathogens2076-08172020-07-019753910.3390/pathogens9070539Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental InfectionCongriev Kumar Kabiraj0Tanjin Tamanna Mumu1Emdadul Haque Chowdhury2Mohammad Rafiqul Islam3Mohammed Nooruzzaman4Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshThe sequential pathology of a genotype XIII Bangladeshi strain of Newcastle disease virus (NDV) was studied in 5-weeks old chickens. Layer chickens of ISA Brown breed were inoculated through the intranasal and intraocular routes with the BD-C161/2010 strain of NDV and examined at different times post-infection (pi). NDV-infected chickens showed depression at 3 days pi (dpi) followed by dropped wings, paralysis and death starting at 4 dpi. Lungs of infected chickens showed hemorrhagic lesions starting at 24 hours pi (hpi) that was followed by pallor and slight contraction by 2 to 3 dpi and subsequently developed into severe hemorrhagic pneumonia with mononuclear cell infiltration. Hemorrhagic and necrotizing lesions were found in different visceral organs including proventriculus, intestine, gut-associated lymphoid tissues, liver and kidneys starting at 3 dpi that progressed rapidly. Severe lymphoid depletion was observed in the thymus, spleen and bursa of Fabricius starting at 1–3 dpi followed by hemorrhages, necrosis, inflammation and atrophy at 4–5 dpi. In the brain, mild neuronal lesions such as focal to diffuse encephalitis with encephalomalacia was observed at 2–3 dpi and moderate and diffuse meningoencephalitis with encephalomalacia at advanced stages. In conclusion, the BD-C161/2010 strain of NDV produced lesions typical of velogenic viscerotropic pathotype of NDV.https://www.mdpi.com/2076-0817/9/7/539newcastle disease virusgenotype XIIIvelogenic pathotypelymphoid depletionmeningoencephalitisBangladesh |
spellingShingle | Congriev Kumar Kabiraj Tanjin Tamanna Mumu Emdadul Haque Chowdhury Mohammad Rafiqul Islam Mohammed Nooruzzaman Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection Pathogens newcastle disease virus genotype XIII velogenic pathotype lymphoid depletion meningoencephalitis Bangladesh |
title | Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection |
title_full | Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection |
title_fullStr | Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection |
title_full_unstemmed | Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection |
title_short | Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection |
title_sort | sequential pathology of a genotype xiii newcastle disease virus from bangladesh in chickens on experimental infection |
topic | newcastle disease virus genotype XIII velogenic pathotype lymphoid depletion meningoencephalitis Bangladesh |
url | https://www.mdpi.com/2076-0817/9/7/539 |
work_keys_str_mv | AT congrievkumarkabiraj sequentialpathologyofagenotypexiiinewcastlediseasevirusfrombangladeshinchickensonexperimentalinfection AT tanjintamannamumu sequentialpathologyofagenotypexiiinewcastlediseasevirusfrombangladeshinchickensonexperimentalinfection AT emdadulhaquechowdhury sequentialpathologyofagenotypexiiinewcastlediseasevirusfrombangladeshinchickensonexperimentalinfection AT mohammadrafiqulislam sequentialpathologyofagenotypexiiinewcastlediseasevirusfrombangladeshinchickensonexperimentalinfection AT mohammednooruzzaman sequentialpathologyofagenotypexiiinewcastlediseasevirusfrombangladeshinchickensonexperimentalinfection |