Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection

The sequential pathology of a genotype XIII Bangladeshi strain of Newcastle disease virus (NDV) was studied in 5-weeks old chickens. Layer chickens of ISA Brown breed were inoculated through the intranasal and intraocular routes with the BD-C161/2010 strain of NDV and examined at different times pos...

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Main Authors: Congriev Kumar Kabiraj, Tanjin Tamanna Mumu, Emdadul Haque Chowdhury, Mohammad Rafiqul Islam, Mohammed Nooruzzaman
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Pathogens
Subjects:
Online Access:https://www.mdpi.com/2076-0817/9/7/539
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author Congriev Kumar Kabiraj
Tanjin Tamanna Mumu
Emdadul Haque Chowdhury
Mohammad Rafiqul Islam
Mohammed Nooruzzaman
author_facet Congriev Kumar Kabiraj
Tanjin Tamanna Mumu
Emdadul Haque Chowdhury
Mohammad Rafiqul Islam
Mohammed Nooruzzaman
author_sort Congriev Kumar Kabiraj
collection DOAJ
description The sequential pathology of a genotype XIII Bangladeshi strain of Newcastle disease virus (NDV) was studied in 5-weeks old chickens. Layer chickens of ISA Brown breed were inoculated through the intranasal and intraocular routes with the BD-C161/2010 strain of NDV and examined at different times post-infection (pi). NDV-infected chickens showed depression at 3 days pi (dpi) followed by dropped wings, paralysis and death starting at 4 dpi. Lungs of infected chickens showed hemorrhagic lesions starting at 24 hours pi (hpi) that was followed by pallor and slight contraction by 2 to 3 dpi and subsequently developed into severe hemorrhagic pneumonia with mononuclear cell infiltration. Hemorrhagic and necrotizing lesions were found in different visceral organs including proventriculus, intestine, gut-associated lymphoid tissues, liver and kidneys starting at 3 dpi that progressed rapidly. Severe lymphoid depletion was observed in the thymus, spleen and bursa of Fabricius starting at 1–3 dpi followed by hemorrhages, necrosis, inflammation and atrophy at 4–5 dpi. In the brain, mild neuronal lesions such as focal to diffuse encephalitis with encephalomalacia was observed at 2–3 dpi and moderate and diffuse meningoencephalitis with encephalomalacia at advanced stages. In conclusion, the BD-C161/2010 strain of NDV produced lesions typical of velogenic viscerotropic pathotype of NDV.
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spelling doaj.art-4f07bcab0c6943719a78fd961e6aaedc2023-11-20T05:58:05ZengMDPI AGPathogens2076-08172020-07-019753910.3390/pathogens9070539Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental InfectionCongriev Kumar Kabiraj0Tanjin Tamanna Mumu1Emdadul Haque Chowdhury2Mohammad Rafiqul Islam3Mohammed Nooruzzaman4Department of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshDepartment of Pathology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, BangladeshThe sequential pathology of a genotype XIII Bangladeshi strain of Newcastle disease virus (NDV) was studied in 5-weeks old chickens. Layer chickens of ISA Brown breed were inoculated through the intranasal and intraocular routes with the BD-C161/2010 strain of NDV and examined at different times post-infection (pi). NDV-infected chickens showed depression at 3 days pi (dpi) followed by dropped wings, paralysis and death starting at 4 dpi. Lungs of infected chickens showed hemorrhagic lesions starting at 24 hours pi (hpi) that was followed by pallor and slight contraction by 2 to 3 dpi and subsequently developed into severe hemorrhagic pneumonia with mononuclear cell infiltration. Hemorrhagic and necrotizing lesions were found in different visceral organs including proventriculus, intestine, gut-associated lymphoid tissues, liver and kidneys starting at 3 dpi that progressed rapidly. Severe lymphoid depletion was observed in the thymus, spleen and bursa of Fabricius starting at 1–3 dpi followed by hemorrhages, necrosis, inflammation and atrophy at 4–5 dpi. In the brain, mild neuronal lesions such as focal to diffuse encephalitis with encephalomalacia was observed at 2–3 dpi and moderate and diffuse meningoencephalitis with encephalomalacia at advanced stages. In conclusion, the BD-C161/2010 strain of NDV produced lesions typical of velogenic viscerotropic pathotype of NDV.https://www.mdpi.com/2076-0817/9/7/539newcastle disease virusgenotype XIIIvelogenic pathotypelymphoid depletionmeningoencephalitisBangladesh
spellingShingle Congriev Kumar Kabiraj
Tanjin Tamanna Mumu
Emdadul Haque Chowdhury
Mohammad Rafiqul Islam
Mohammed Nooruzzaman
Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection
Pathogens
newcastle disease virus
genotype XIII
velogenic pathotype
lymphoid depletion
meningoencephalitis
Bangladesh
title Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection
title_full Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection
title_fullStr Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection
title_full_unstemmed Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection
title_short Sequential Pathology of a Genotype XIII Newcastle Disease Virus from Bangladesh in Chickens on Experimental Infection
title_sort sequential pathology of a genotype xiii newcastle disease virus from bangladesh in chickens on experimental infection
topic newcastle disease virus
genotype XIII
velogenic pathotype
lymphoid depletion
meningoencephalitis
Bangladesh
url https://www.mdpi.com/2076-0817/9/7/539
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