Antiviral and immunoregulatory effects of curcumin on coxsackievirus B3-infected hepatitis
Fulminant hepatitis is a life-threatening complication of coxsackievirus B (CVB) 3 infections. The condition may deteriorate to disseminated intravascular coagulopathy with markedly increased liver enzymes, inflammatory cytokines, and chemokines, which significantly induce local and systemic inflamm...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Virus Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S016817022300165X |
| _version_ | 1797666024980480000 |
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| author | Yu-Jheng Lin Li-Chiu Wang Huey-Pin Tsai Chia-Yu Chi Chih-Peng Chang Shun-Hua Chen Shih-Min Wang |
| author_facet | Yu-Jheng Lin Li-Chiu Wang Huey-Pin Tsai Chia-Yu Chi Chih-Peng Chang Shun-Hua Chen Shih-Min Wang |
| author_sort | Yu-Jheng Lin |
| collection | DOAJ |
| description | Fulminant hepatitis is a life-threatening complication of coxsackievirus B (CVB) 3 infections. The condition may deteriorate to disseminated intravascular coagulopathy with markedly increased liver enzymes, inflammatory cytokines, and chemokines, which significantly induce local and systemic inflammation. Curcumin exhibits anti-inflammatory and antiviral characteristics in inflammatory and infectious diseases. Here we determined effects of curcumin on viral replications, cytokine and chemokine expressions, and liver damage in CVB3-infected Huh-7 cells. The mouse-adapted CVB3 strain was used to investigate the antiviral and anti-inflammatory effects of curcumin on CVB3-induced hepatitis in a mouse model. In vitro studies showed that curcumin reduced viral protein and titer levels and increased cell viability. Curcumin enhanced the heme oxygenase-1 (HO-1) protein level and decreased the levels of cleaved caspase-3 protein and mRNA of gene encoding C-X-C motif chemokine 10 in infected cells. In vivo studies showed that curcumin improved the survival rate and clinical scores in mice and reduced the viral titer in the liver during CVB3 infection. Moreover, the HO-1 levels were increased, and the cleaved caspase-3 levels were diminished in the CVB3-infected liver. Curcumin reduced the levels of interferon (IFN)-γ and monokine induced by IFN-γ in liver and levels of interleukin (IL)-8 in serum, but increased levels of regulated activation, normal T cell expression in liver and levels of IL-10 in serum of CVB3-infected mice. In summary, curcumin presents antiviral and anti-inflammation efficacies in CVB3 infection in vitro and in vivo; these results provide potential evidence on the feasibility of curcumin for clinical treatment. |
| first_indexed | 2024-03-11T19:52:15Z |
| format | Article |
| id | doaj.art-4f0d40ff528a4308a42630dbe16e0ec6 |
| institution | Directory Open Access Journal |
| issn | 1872-7492 |
| language | English |
| last_indexed | 2024-03-11T19:52:15Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Virus Research |
| spelling | doaj.art-4f0d40ff528a4308a42630dbe16e0ec62023-10-05T04:22:41ZengElsevierVirus Research1872-74922023-10-01336199203Antiviral and immunoregulatory effects of curcumin on coxsackievirus B3-infected hepatitisYu-Jheng Lin0Li-Chiu Wang1Huey-Pin Tsai2Chia-Yu Chi3Chih-Peng Chang4Shun-Hua Chen5Shih-Min Wang6Department of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan 70101, TaiwanSchool of Medicine, I-Shou University, Kaohsiung 82445, TaiwanDepartment of Pathology,College of Medicine, National Cheng Kung University Hospital, National Cheng Kung University, Tainan 70401, Taiwan; Department of Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng Kung University, Tainan 70401, TaiwanDepartment of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan; National Institute of Infectious Diseases and Vaccinology, National Health Research Institutes, Miaoli County 35053, TaiwanDepartment of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan; Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan 70401, Taiwan; Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 70401, TaiwanDepartment of Microbiology & Immunology, College of Medicine, National Cheng Kung University, Tainan 70101, Taiwan; Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan 70401, Taiwan; Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan 70401, Taiwan; Corresponding authors at: Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan 70401, Taiwan.Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan 70401, Taiwan; Center for Infection Control, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, 70401, Taiwan; Corresponding authors at: Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan 70401, Taiwan.Fulminant hepatitis is a life-threatening complication of coxsackievirus B (CVB) 3 infections. The condition may deteriorate to disseminated intravascular coagulopathy with markedly increased liver enzymes, inflammatory cytokines, and chemokines, which significantly induce local and systemic inflammation. Curcumin exhibits anti-inflammatory and antiviral characteristics in inflammatory and infectious diseases. Here we determined effects of curcumin on viral replications, cytokine and chemokine expressions, and liver damage in CVB3-infected Huh-7 cells. The mouse-adapted CVB3 strain was used to investigate the antiviral and anti-inflammatory effects of curcumin on CVB3-induced hepatitis in a mouse model. In vitro studies showed that curcumin reduced viral protein and titer levels and increased cell viability. Curcumin enhanced the heme oxygenase-1 (HO-1) protein level and decreased the levels of cleaved caspase-3 protein and mRNA of gene encoding C-X-C motif chemokine 10 in infected cells. In vivo studies showed that curcumin improved the survival rate and clinical scores in mice and reduced the viral titer in the liver during CVB3 infection. Moreover, the HO-1 levels were increased, and the cleaved caspase-3 levels were diminished in the CVB3-infected liver. Curcumin reduced the levels of interferon (IFN)-γ and monokine induced by IFN-γ in liver and levels of interleukin (IL)-8 in serum, but increased levels of regulated activation, normal T cell expression in liver and levels of IL-10 in serum of CVB3-infected mice. In summary, curcumin presents antiviral and anti-inflammation efficacies in CVB3 infection in vitro and in vivo; these results provide potential evidence on the feasibility of curcumin for clinical treatment.http://www.sciencedirect.com/science/article/pii/S016817022300165XCoxsackievirus BHepatitisCurcuminCytokineHeme oxygenase-1 |
| spellingShingle | Yu-Jheng Lin Li-Chiu Wang Huey-Pin Tsai Chia-Yu Chi Chih-Peng Chang Shun-Hua Chen Shih-Min Wang Antiviral and immunoregulatory effects of curcumin on coxsackievirus B3-infected hepatitis Virus Research Coxsackievirus B Hepatitis Curcumin Cytokine Heme oxygenase-1 |
| title | Antiviral and immunoregulatory effects of curcumin on coxsackievirus B3-infected hepatitis |
| title_full | Antiviral and immunoregulatory effects of curcumin on coxsackievirus B3-infected hepatitis |
| title_fullStr | Antiviral and immunoregulatory effects of curcumin on coxsackievirus B3-infected hepatitis |
| title_full_unstemmed | Antiviral and immunoregulatory effects of curcumin on coxsackievirus B3-infected hepatitis |
| title_short | Antiviral and immunoregulatory effects of curcumin on coxsackievirus B3-infected hepatitis |
| title_sort | antiviral and immunoregulatory effects of curcumin on coxsackievirus b3 infected hepatitis |
| topic | Coxsackievirus B Hepatitis Curcumin Cytokine Heme oxygenase-1 |
| url | http://www.sciencedirect.com/science/article/pii/S016817022300165X |
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