Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosisResearch in context
Background: Histone deacetylase 4 (HDAC4) has been proposed as a target for Amyotrophic Lateral Sclerosis (ALS) because it mediates nerve-skeletal muscle interaction and since its expression in skeletal muscle correlates with the severity of the disease. However, our recent studies on the skeletal m...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2019-02-01
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| Series: | EBioMedicine |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352396419300441 |
| _version_ | 1819241139593543680 |
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| author | Eva Pigna Elena Simonazzi Krizia Sanna Krzysztof Marian Bernadzki Tomek Proszynski Constantin Heil Daniela Palacios Sergio Adamo Viviana Moresi |
| author_facet | Eva Pigna Elena Simonazzi Krizia Sanna Krzysztof Marian Bernadzki Tomek Proszynski Constantin Heil Daniela Palacios Sergio Adamo Viviana Moresi |
| author_sort | Eva Pigna |
| collection | DOAJ |
| description | Background: Histone deacetylase 4 (HDAC4) has been proposed as a target for Amyotrophic Lateral Sclerosis (ALS) because it mediates nerve-skeletal muscle interaction and since its expression in skeletal muscle correlates with the severity of the disease. However, our recent studies on the skeletal muscle response upon long-term denervation highlighted the importance of HDAC4 in maintaining muscle integrity. Methods: To fully identify the yet uncharacterized HDAC4 functions in ALS, we genetically deleted HDAC4 in skeletal muscles of a mouse model of ALS. Body weight, skeletal muscle, innervation and spinal cord were analyzed over time by morphological and molecular analyses. Transcriptome analysis was also performed to delineate the signaling modulated by HDAC4 in skeletal muscle of a mouse model of ALS. Findings: HDAC4 deletion in skeletal muscle caused earlier ALS onset, characterized by body weight loss, muscle denervation and atrophy, and compromised muscle performance, although the main catabolic pathways were not activated. Transcriptome analysis identified the gene networks modulated by HDAC4 in ALS, revealing UCP1 as a top regulator that may be implicated in worsening ALS features. Interpretation: HDAC4 plays an important role in preserving innervations and skeletal muscle in ALS, likely by modulating the UCP1 gene network. Our study highlights a possible risk in considering HDAC inhibitors for the treatment of ALS. Fund: This work was supported by FIRB grant (RBFR12BUMH) from Ministry of Education, Universities and Research, by Fondazione Veronesi, by Sapienza research project 2017 (RM11715C78539BD8) and Polish National Science Center grant (UMO-2016/21/B/NZ3/03638). Keywords: HDAC4, HDAC inhibitors, SOD1G93A mice, Epigenetics, ALS |
| first_indexed | 2024-12-23T14:19:10Z |
| format | Article |
| id | doaj.art-4f0df91a0ba54f9ba7cda7a5a676e4d9 |
| institution | Directory Open Access Journal |
| issn | 2352-3964 |
| language | English |
| last_indexed | 2024-12-23T14:19:10Z |
| publishDate | 2019-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | EBioMedicine |
| spelling | doaj.art-4f0df91a0ba54f9ba7cda7a5a676e4d92022-12-21T17:43:50ZengElsevierEBioMedicine2352-39642019-02-0140717732Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosisResearch in contextEva Pigna0Elena Simonazzi1Krizia Sanna2Krzysztof Marian Bernadzki3Tomek Proszynski4Constantin Heil5Daniela Palacios6Sergio Adamo7Viviana Moresi8DAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Via Antonio Scarpa 16, 00161 Rome, ItalyDAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Via Antonio Scarpa 16, 00161 Rome, ItalyDAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Via Antonio Scarpa 16, 00161 Rome, ItalyLaboratory of Synaptogenesis, Nencki Institute of Experimental Biology Polish Academy of Sciences, Pasteur 3, 02-093 Warsaw, PolandLaboratory of Synaptogenesis, Nencki Institute of Experimental Biology Polish Academy of Sciences, Pasteur 3, 02-093 Warsaw, PolandIRCCS Fondazione Santa Lucia, Via del Fosso di Fiorano 64, 00143 Rome, ItalyIRCCS Fondazione Santa Lucia, Via del Fosso di Fiorano 64, 00143 Rome, ItalyDAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Via Antonio Scarpa 16, 00161 Rome, ItalyDAHFMO Unit of Histology and Medical Embryology, Interuniversity Institute of Myology, Sapienza University of Rome, Via Antonio Scarpa 16, 00161 Rome, Italy; Corresponding author.Background: Histone deacetylase 4 (HDAC4) has been proposed as a target for Amyotrophic Lateral Sclerosis (ALS) because it mediates nerve-skeletal muscle interaction and since its expression in skeletal muscle correlates with the severity of the disease. However, our recent studies on the skeletal muscle response upon long-term denervation highlighted the importance of HDAC4 in maintaining muscle integrity. Methods: To fully identify the yet uncharacterized HDAC4 functions in ALS, we genetically deleted HDAC4 in skeletal muscles of a mouse model of ALS. Body weight, skeletal muscle, innervation and spinal cord were analyzed over time by morphological and molecular analyses. Transcriptome analysis was also performed to delineate the signaling modulated by HDAC4 in skeletal muscle of a mouse model of ALS. Findings: HDAC4 deletion in skeletal muscle caused earlier ALS onset, characterized by body weight loss, muscle denervation and atrophy, and compromised muscle performance, although the main catabolic pathways were not activated. Transcriptome analysis identified the gene networks modulated by HDAC4 in ALS, revealing UCP1 as a top regulator that may be implicated in worsening ALS features. Interpretation: HDAC4 plays an important role in preserving innervations and skeletal muscle in ALS, likely by modulating the UCP1 gene network. Our study highlights a possible risk in considering HDAC inhibitors for the treatment of ALS. Fund: This work was supported by FIRB grant (RBFR12BUMH) from Ministry of Education, Universities and Research, by Fondazione Veronesi, by Sapienza research project 2017 (RM11715C78539BD8) and Polish National Science Center grant (UMO-2016/21/B/NZ3/03638). Keywords: HDAC4, HDAC inhibitors, SOD1G93A mice, Epigenetics, ALShttp://www.sciencedirect.com/science/article/pii/S2352396419300441 |
| spellingShingle | Eva Pigna Elena Simonazzi Krizia Sanna Krzysztof Marian Bernadzki Tomek Proszynski Constantin Heil Daniela Palacios Sergio Adamo Viviana Moresi Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosisResearch in context EBioMedicine |
| title | Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosisResearch in context |
| title_full | Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosisResearch in context |
| title_fullStr | Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosisResearch in context |
| title_full_unstemmed | Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosisResearch in context |
| title_short | Histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosisResearch in context |
| title_sort | histone deacetylase 4 protects from denervation and skeletal muscle atrophy in a murine model of amyotrophic lateral sclerosisresearch in context |
| url | http://www.sciencedirect.com/science/article/pii/S2352396419300441 |
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