Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice
Understanding the tissue changes and molecular mechanisms of preclinical models is essential for creating an optimal experimental design for credible translation into clinics. In our study, a chlorhexidine (CHX)-induced mouse model of peritoneal fibrosis was used to analyze histological and molecula...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/11/2726 |
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| author | Neža Brezovec Nika Kojc Andreja Erman Matjaž Hladnik Jošt Stergar Matija Milanič Matija Tomšič Saša Čučnik Snežna Sodin-Šemrl Martina Perše Katja Lakota |
| author_facet | Neža Brezovec Nika Kojc Andreja Erman Matjaž Hladnik Jošt Stergar Matija Milanič Matija Tomšič Saša Čučnik Snežna Sodin-Šemrl Martina Perše Katja Lakota |
| author_sort | Neža Brezovec |
| collection | DOAJ |
| description | Understanding the tissue changes and molecular mechanisms of preclinical models is essential for creating an optimal experimental design for credible translation into clinics. In our study, a chlorhexidine (CHX)-induced mouse model of peritoneal fibrosis was used to analyze histological and molecular/cellular alterations induced by 1 and 3 weeks of intraperitoneal CHX application. CHX treatment for 1 week already caused injury, degradation, and loss of mesothelial cells, resulting in local inflammation, with the most severe structural changes occurring in the peritoneum around the ventral parts of the abdominal wall. The local inflammatory response in the abdominal wall showed no prominent differences between 1 and 3 weeks. We observed an increase in polymorphonuclear cells in the blood but no evidence of systemic inflammation as measured by serum levels of serum amyloid A and interleukin-6. CHX-induced fibrosis in the abdominal wall was more pronounced after 3 weeks, but the gene expression of fibrotic markers did not change over time. Complement system molecules were strongly expressed in the abdominal wall of CHX-treated mice. To conclude, both histological and molecular changes were already present in week 1, allowing examination at the onset of fibrosis. This is crucial information for refining further experiments and limiting the amount of unnecessary animal suffering. |
| first_indexed | 2024-03-09T19:15:04Z |
| format | Article |
| id | doaj.art-4f0f2d788e074e7a9163d49fb56e3441 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T19:15:04Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-4f0f2d788e074e7a9163d49fb56e34412023-11-24T03:49:42ZengMDPI AGBiomedicines2227-90592022-10-011011272610.3390/biomedicines10112726Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in MiceNeža Brezovec0Nika Kojc1Andreja Erman2Matjaž Hladnik3Jošt Stergar4Matija Milanič5Matija Tomšič6Saša Čučnik7Snežna Sodin-Šemrl8Martina Perše9Katja Lakota10Department of Rheumatology, University Medical Centre Ljubljana, 1000 Ljubljana, SloveniaFaculty of Medicine, University of Ljubljana, 1000 Ljubljana, SloveniaInstitute of Cell Biology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, SloveniaFaculty of Mathematics, Natural Sciences and Information Technologies, University of Primorska, 6000 Koper, SloveniaReactor Physics Department, Jožef Stefan Institute, 1000 Ljubljana, SloveniaReactor Physics Department, Jožef Stefan Institute, 1000 Ljubljana, SloveniaDepartment of Rheumatology, University Medical Centre Ljubljana, 1000 Ljubljana, SloveniaDepartment of Rheumatology, University Medical Centre Ljubljana, 1000 Ljubljana, SloveniaDepartment of Rheumatology, University Medical Centre Ljubljana, 1000 Ljubljana, SloveniaFaculty of Medicine, University of Ljubljana, 1000 Ljubljana, SloveniaDepartment of Rheumatology, University Medical Centre Ljubljana, 1000 Ljubljana, SloveniaUnderstanding the tissue changes and molecular mechanisms of preclinical models is essential for creating an optimal experimental design for credible translation into clinics. In our study, a chlorhexidine (CHX)-induced mouse model of peritoneal fibrosis was used to analyze histological and molecular/cellular alterations induced by 1 and 3 weeks of intraperitoneal CHX application. CHX treatment for 1 week already caused injury, degradation, and loss of mesothelial cells, resulting in local inflammation, with the most severe structural changes occurring in the peritoneum around the ventral parts of the abdominal wall. The local inflammatory response in the abdominal wall showed no prominent differences between 1 and 3 weeks. We observed an increase in polymorphonuclear cells in the blood but no evidence of systemic inflammation as measured by serum levels of serum amyloid A and interleukin-6. CHX-induced fibrosis in the abdominal wall was more pronounced after 3 weeks, but the gene expression of fibrotic markers did not change over time. Complement system molecules were strongly expressed in the abdominal wall of CHX-treated mice. To conclude, both histological and molecular changes were already present in week 1, allowing examination at the onset of fibrosis. This is crucial information for refining further experiments and limiting the amount of unnecessary animal suffering.https://www.mdpi.com/2227-9059/10/11/2726chlorhexidine gluconateperitoneal fibrosismouse modelinflammationcomplement |
| spellingShingle | Neža Brezovec Nika Kojc Andreja Erman Matjaž Hladnik Jošt Stergar Matija Milanič Matija Tomšič Saša Čučnik Snežna Sodin-Šemrl Martina Perše Katja Lakota Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice Biomedicines chlorhexidine gluconate peritoneal fibrosis mouse model inflammation complement |
| title | Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice |
| title_full | Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice |
| title_fullStr | Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice |
| title_full_unstemmed | Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice |
| title_short | Molecular and Cellular Markers in Chlorhexidine-Induced Peritoneal Fibrosis in Mice |
| title_sort | molecular and cellular markers in chlorhexidine induced peritoneal fibrosis in mice |
| topic | chlorhexidine gluconate peritoneal fibrosis mouse model inflammation complement |
| url | https://www.mdpi.com/2227-9059/10/11/2726 |
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