Characterization and Optimization of Culture Conditions for <i>Aurantiochytrium</i> sp. SC145 Isolated from Sand Cay (Son Ca) Island, Vietnam, and Antioxidative and Neuroprotective Activities of Its Polyunsaturated Fatty Acid Mixture
<i>Aurantiochytrium</i> is a heterotrophic marine microalga that has potential industrial applications. The main objectives of this study were to isolate an <i>Aurantiochytrium</i> strain from Sand Cay (Son Ca) Island, Vietnam, optimize its culture conditions, determine its n...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/12/780 |
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| author | Hoang Thi Minh Hien Le Thi Thom Nguyen Cam Ha Luu Thi Tam Ngo Thi Hoai Thu Tru Van Nguyen Vu Thi Loan Nguyen Trong Dan Dang Diem Hong |
| author_facet | Hoang Thi Minh Hien Le Thi Thom Nguyen Cam Ha Luu Thi Tam Ngo Thi Hoai Thu Tru Van Nguyen Vu Thi Loan Nguyen Trong Dan Dang Diem Hong |
| author_sort | Hoang Thi Minh Hien |
| collection | DOAJ |
| description | <i>Aurantiochytrium</i> is a heterotrophic marine microalga that has potential industrial applications. The main objectives of this study were to isolate an <i>Aurantiochytrium</i> strain from Sand Cay (Son Ca) Island, Vietnam, optimize its culture conditions, determine its nutritional composition, extract polyunsaturated fatty acids (PUFAs) in the free (FFA) and the alkyl ester (FAAE) forms, and evaluate the antioxidation and neuroprotection properties of the PUFAs. <i>Aurantiochytrium</i> sp. SC145 can be grown stably under laboratory conditions. Its culture conditions were optimized for a dry cell weight (DCW) of 31.18 g/L, with total lipids comprising 25.29%, proteins 7.93%, carbohydrates 15.21%, and carotenoid at 143.67 µg/L of DCW. The FAAEs and FFAs extracted from <i>Aurantiochytrium</i> sp. SC145 were rich in omega 3–6–9 fatty acids (40.73% and 44.00% of total fatty acids, respectively). No acute or subchronic oral toxicity was determined in mice fed with the PUFAs in FFA or FAAE forms at different doses over 90 days. Furthermore, the PUFAs in the FFA or FAAE forms and their main constituents of EPA, DHA, and ALA showed antioxidant and AChE inhibitory properties and neuroprotective activities against damage caused by H<sub>2</sub>O<sub>2</sub>- and amyloid-ß protein fragment 25–35 (Aβ<sub>25-35</sub>)-induced C6 cells. These data suggest that PUFAs extracted from <i>Aurantiochytrium</i> sp. SC145 may be a potential therapeutic target for the treatment of neurodegenerative disorders. |
| first_indexed | 2024-03-09T16:09:55Z |
| format | Article |
| id | doaj.art-4f0f7ba0b9e4497a919919c0f4a7761b |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-09T16:09:55Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-4f0f7ba0b9e4497a919919c0f4a7761b2023-11-24T16:20:04ZengMDPI AGMarine Drugs1660-33972022-12-01201278010.3390/md20120780Characterization and Optimization of Culture Conditions for <i>Aurantiochytrium</i> sp. SC145 Isolated from Sand Cay (Son Ca) Island, Vietnam, and Antioxidative and Neuroprotective Activities of Its Polyunsaturated Fatty Acid MixtureHoang Thi Minh Hien0Le Thi Thom1Nguyen Cam Ha2Luu Thi Tam3Ngo Thi Hoai Thu4Tru Van Nguyen5Vu Thi Loan6Nguyen Trong Dan7Dang Diem Hong8Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, VietnamJoint Vietnam–Russia Tropical Science and Technology Research Center, 63 Nguyen Van Huyen Str., Cau Giay, Hanoi 100000, VietnamJoint Vietnam–Russia Tropical Science and Technology Research Center, 63 Nguyen Van Huyen Str., Cau Giay, Hanoi 100000, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam<i>Aurantiochytrium</i> is a heterotrophic marine microalga that has potential industrial applications. The main objectives of this study were to isolate an <i>Aurantiochytrium</i> strain from Sand Cay (Son Ca) Island, Vietnam, optimize its culture conditions, determine its nutritional composition, extract polyunsaturated fatty acids (PUFAs) in the free (FFA) and the alkyl ester (FAAE) forms, and evaluate the antioxidation and neuroprotection properties of the PUFAs. <i>Aurantiochytrium</i> sp. SC145 can be grown stably under laboratory conditions. Its culture conditions were optimized for a dry cell weight (DCW) of 31.18 g/L, with total lipids comprising 25.29%, proteins 7.93%, carbohydrates 15.21%, and carotenoid at 143.67 µg/L of DCW. The FAAEs and FFAs extracted from <i>Aurantiochytrium</i> sp. SC145 were rich in omega 3–6–9 fatty acids (40.73% and 44.00% of total fatty acids, respectively). No acute or subchronic oral toxicity was determined in mice fed with the PUFAs in FFA or FAAE forms at different doses over 90 days. Furthermore, the PUFAs in the FFA or FAAE forms and their main constituents of EPA, DHA, and ALA showed antioxidant and AChE inhibitory properties and neuroprotective activities against damage caused by H<sub>2</sub>O<sub>2</sub>- and amyloid-ß protein fragment 25–35 (Aβ<sub>25-35</sub>)-induced C6 cells. These data suggest that PUFAs extracted from <i>Aurantiochytrium</i> sp. SC145 may be a potential therapeutic target for the treatment of neurodegenerative disorders.https://www.mdpi.com/1660-3397/20/12/780Antioxidant and acetylcholinesterase (AChE) inhibitorfatty acid in the free formfatty acid in the alkyl ester formheterotrophic marine microalganeuroprotective effect |
| spellingShingle | Hoang Thi Minh Hien Le Thi Thom Nguyen Cam Ha Luu Thi Tam Ngo Thi Hoai Thu Tru Van Nguyen Vu Thi Loan Nguyen Trong Dan Dang Diem Hong Characterization and Optimization of Culture Conditions for <i>Aurantiochytrium</i> sp. SC145 Isolated from Sand Cay (Son Ca) Island, Vietnam, and Antioxidative and Neuroprotective Activities of Its Polyunsaturated Fatty Acid Mixture Marine Drugs Antioxidant and acetylcholinesterase (AChE) inhibitor fatty acid in the free form fatty acid in the alkyl ester form heterotrophic marine microalga neuroprotective effect |
| title | Characterization and Optimization of Culture Conditions for <i>Aurantiochytrium</i> sp. SC145 Isolated from Sand Cay (Son Ca) Island, Vietnam, and Antioxidative and Neuroprotective Activities of Its Polyunsaturated Fatty Acid Mixture |
| title_full | Characterization and Optimization of Culture Conditions for <i>Aurantiochytrium</i> sp. SC145 Isolated from Sand Cay (Son Ca) Island, Vietnam, and Antioxidative and Neuroprotective Activities of Its Polyunsaturated Fatty Acid Mixture |
| title_fullStr | Characterization and Optimization of Culture Conditions for <i>Aurantiochytrium</i> sp. SC145 Isolated from Sand Cay (Son Ca) Island, Vietnam, and Antioxidative and Neuroprotective Activities of Its Polyunsaturated Fatty Acid Mixture |
| title_full_unstemmed | Characterization and Optimization of Culture Conditions for <i>Aurantiochytrium</i> sp. SC145 Isolated from Sand Cay (Son Ca) Island, Vietnam, and Antioxidative and Neuroprotective Activities of Its Polyunsaturated Fatty Acid Mixture |
| title_short | Characterization and Optimization of Culture Conditions for <i>Aurantiochytrium</i> sp. SC145 Isolated from Sand Cay (Son Ca) Island, Vietnam, and Antioxidative and Neuroprotective Activities of Its Polyunsaturated Fatty Acid Mixture |
| title_sort | characterization and optimization of culture conditions for i aurantiochytrium i sp sc145 isolated from sand cay son ca island vietnam and antioxidative and neuroprotective activities of its polyunsaturated fatty acid mixture |
| topic | Antioxidant and acetylcholinesterase (AChE) inhibitor fatty acid in the free form fatty acid in the alkyl ester form heterotrophic marine microalga neuroprotective effect |
| url | https://www.mdpi.com/1660-3397/20/12/780 |
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