Comparative Genomics and In Vitro Plant Growth Promotion and Biocontrol Traits of Lactic Acid Bacteria from the Wheat Rhizosphere
This study aimed to isolate lactic acid bacteria (LAB) from wheat rhizosphere, to characterize their in vitro plant growth promoting activities and to differentiate plant-associated LAB from those associated with foods or human disease through comparative genomic analysis. <i>Lactococcus lacti...
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MDPI AG
2020-12-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/1/78 |
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| author | Sabrina Strafella David J. Simpson Mohammad Yaghoubi Khanghahi Maria De Angelis Michael Gänzle Fabio Minervini Carmine Crecchio |
| author_facet | Sabrina Strafella David J. Simpson Mohammad Yaghoubi Khanghahi Maria De Angelis Michael Gänzle Fabio Minervini Carmine Crecchio |
| author_sort | Sabrina Strafella |
| collection | DOAJ |
| description | This study aimed to isolate lactic acid bacteria (LAB) from wheat rhizosphere, to characterize their in vitro plant growth promoting activities and to differentiate plant-associated LAB from those associated with foods or human disease through comparative genomic analysis. <i>Lactococcus lactis</i> subsp. <i>lactis</i> and <i>Enterococcus faecium</i> were isolated using de Man-Rogosa-Sharpe (MRS) and Glucose Yeast Peptone (GYP) as enrichment culture media. Comparative genomic analyses showed that plant-associated LAB strains were enriched in genes coding for bacteriocin production when compared to strains from other ecosystems. Isolates of <i>L. lactis</i> and <i>E. faecium</i> did not produce physiologically relevant concentrations of the phyto-hormone indolacetic acid. All isolates solubilized high amount of phosphate and 12 of 16 strains solubilized potassium. <i>E. faecium</i> LB5, <i>L. lactis</i> LB6, LB7, and LB9 inhibited the plant pathogenic <i>Fusarium graminearum</i> to the same extent as two strains of <i>Bacillus</i> sp. However, the antifungal activity of the abovementioned LAB strains depended on the medium of cultivation and a low pH while antifungal activity of <i>Bacillus</i> spp. was independent of the growth medium and likely relates to antifungal lipopeptides. This study showed the potential of rhizospheric LAB for future application as biofertilizers in agriculture. |
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| publishDate | 2020-12-01 |
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| series | Microorganisms |
| spelling | doaj.art-4f118a6143fa4e9ab6989f0e410541cf2023-11-21T03:07:25ZengMDPI AGMicroorganisms2076-26072020-12-01917810.3390/microorganisms9010078Comparative Genomics and In Vitro Plant Growth Promotion and Biocontrol Traits of Lactic Acid Bacteria from the Wheat RhizosphereSabrina Strafella0David J. Simpson1Mohammad Yaghoubi Khanghahi2Maria De Angelis3Michael Gänzle4Fabio Minervini5Carmine Crecchio6Department of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Via Amendola 165/a, 70126 Bari, ItalyDepartment of Agricultural, Food and Nutritional Science, 410 Agriculture/Forestry Centre, University of Alberta, Edmonton, AB T6G 2P5, CanadaDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Via Amendola 165/a, 70126 Bari, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Via Amendola 165/a, 70126 Bari, ItalyDepartment of Agricultural, Food and Nutritional Science, 410 Agriculture/Forestry Centre, University of Alberta, Edmonton, AB T6G 2P5, CanadaDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Via Amendola 165/a, 70126 Bari, ItalyDepartment of Soil, Plant and Food Sciences, University of Bari Aldo Moro, Via Amendola 165/a, 70126 Bari, ItalyThis study aimed to isolate lactic acid bacteria (LAB) from wheat rhizosphere, to characterize their in vitro plant growth promoting activities and to differentiate plant-associated LAB from those associated with foods or human disease through comparative genomic analysis. <i>Lactococcus lactis</i> subsp. <i>lactis</i> and <i>Enterococcus faecium</i> were isolated using de Man-Rogosa-Sharpe (MRS) and Glucose Yeast Peptone (GYP) as enrichment culture media. Comparative genomic analyses showed that plant-associated LAB strains were enriched in genes coding for bacteriocin production when compared to strains from other ecosystems. Isolates of <i>L. lactis</i> and <i>E. faecium</i> did not produce physiologically relevant concentrations of the phyto-hormone indolacetic acid. All isolates solubilized high amount of phosphate and 12 of 16 strains solubilized potassium. <i>E. faecium</i> LB5, <i>L. lactis</i> LB6, LB7, and LB9 inhibited the plant pathogenic <i>Fusarium graminearum</i> to the same extent as two strains of <i>Bacillus</i> sp. However, the antifungal activity of the abovementioned LAB strains depended on the medium of cultivation and a low pH while antifungal activity of <i>Bacillus</i> spp. was independent of the growth medium and likely relates to antifungal lipopeptides. This study showed the potential of rhizospheric LAB for future application as biofertilizers in agriculture.https://www.mdpi.com/2076-2607/9/1/78plant growth promoting bacterialactic acid bacteriawheat rhizospheric soilcomparative genomic analysisbacteriocins3-indolacetic acid production |
| spellingShingle | Sabrina Strafella David J. Simpson Mohammad Yaghoubi Khanghahi Maria De Angelis Michael Gänzle Fabio Minervini Carmine Crecchio Comparative Genomics and In Vitro Plant Growth Promotion and Biocontrol Traits of Lactic Acid Bacteria from the Wheat Rhizosphere Microorganisms plant growth promoting bacteria lactic acid bacteria wheat rhizospheric soil comparative genomic analysis bacteriocins 3-indolacetic acid production |
| title | Comparative Genomics and In Vitro Plant Growth Promotion and Biocontrol Traits of Lactic Acid Bacteria from the Wheat Rhizosphere |
| title_full | Comparative Genomics and In Vitro Plant Growth Promotion and Biocontrol Traits of Lactic Acid Bacteria from the Wheat Rhizosphere |
| title_fullStr | Comparative Genomics and In Vitro Plant Growth Promotion and Biocontrol Traits of Lactic Acid Bacteria from the Wheat Rhizosphere |
| title_full_unstemmed | Comparative Genomics and In Vitro Plant Growth Promotion and Biocontrol Traits of Lactic Acid Bacteria from the Wheat Rhizosphere |
| title_short | Comparative Genomics and In Vitro Plant Growth Promotion and Biocontrol Traits of Lactic Acid Bacteria from the Wheat Rhizosphere |
| title_sort | comparative genomics and in vitro plant growth promotion and biocontrol traits of lactic acid bacteria from the wheat rhizosphere |
| topic | plant growth promoting bacteria lactic acid bacteria wheat rhizospheric soil comparative genomic analysis bacteriocins 3-indolacetic acid production |
| url | https://www.mdpi.com/2076-2607/9/1/78 |
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