NMDA GluN2C/2D receptors contribute to synaptic regulation and plasticity in the anterior cingulate cortex of adult mice
Abstract Introduction N-Methyl-D-aspartate receptors (NMDARs) play a critical role in different forms of plasticity in the central nervous system. NMDARs are always assembled in tetrameric form, in which two GluN1 subunits and two GluN2 and/or GluN3 subunits combine together. Previous studies focuse...
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| Format: | Article |
| Language: | English |
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BMC
2021-03-01
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| Series: | Molecular Brain |
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| Online Access: | https://doi.org/10.1186/s13041-021-00744-3 |
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| author | Qi-Yu Chen Xu-Hui Li Jing-Shan Lu Yinglu Liu Jung-Hyun Alex Lee Yu-Xin Chen Wantong Shi Kexin Fan Min Zhuo |
| author_facet | Qi-Yu Chen Xu-Hui Li Jing-Shan Lu Yinglu Liu Jung-Hyun Alex Lee Yu-Xin Chen Wantong Shi Kexin Fan Min Zhuo |
| author_sort | Qi-Yu Chen |
| collection | DOAJ |
| description | Abstract Introduction N-Methyl-D-aspartate receptors (NMDARs) play a critical role in different forms of plasticity in the central nervous system. NMDARs are always assembled in tetrameric form, in which two GluN1 subunits and two GluN2 and/or GluN3 subunits combine together. Previous studies focused mainly on the hippocampus. The anterior cingulate cortex (ACC) is a key cortical region for sensory and emotional functions. NMDAR GluN2A and GluN2B subunits have been previously investigated, however much less is known about the GluN2C/2D subunits. Results In the present study, we found that the GluN2C/2D subunits are expressed in the pyramidal cells of ACC of adult mice. Application of a selective antagonist of GluN2C/2D, (2R*,3S*)-1-(9-bromophenanthrene-3-carbonyl) piperazine-2,3-dicarboxylic acid (UBP145), significantly reduced NMDAR-mediated currents, while synaptically evoked EPSCs were not affected. UBP145 affected neither the postsynaptic long-term potentiation (post-LTP) nor the presynaptic LTP (pre-LTP). Furthermore, the long-term depression (LTD) was also not affected by UBP145. Finally, both UBP145 decreased the frequency of the miniature EPSCs (mEPSCs) while the amplitude remained intact, suggesting that the GluN2C/2D may be involved in presynaptic regulation of spontaneous glutamate release. Conclusions Our results provide direct evidence that the GluN2C/2D contributes to evoked NMDAR mediated currents and mEPSCs in the ACC, which may have significant physiological implications. |
| first_indexed | 2024-12-20T13:00:37Z |
| format | Article |
| id | doaj.art-4f150ea282d74aacbd70d66abde5cd01 |
| institution | Directory Open Access Journal |
| issn | 1756-6606 |
| language | English |
| last_indexed | 2024-12-20T13:00:37Z |
| publishDate | 2021-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Brain |
| spelling | doaj.art-4f150ea282d74aacbd70d66abde5cd012022-12-21T19:39:56ZengBMCMolecular Brain1756-66062021-03-0114111310.1186/s13041-021-00744-3NMDA GluN2C/2D receptors contribute to synaptic regulation and plasticity in the anterior cingulate cortex of adult miceQi-Yu Chen0Xu-Hui Li1Jing-Shan Lu2Yinglu Liu3Jung-Hyun Alex Lee4Yu-Xin Chen5Wantong Shi6Kexin Fan7Min Zhuo8Center for Neuron and Disease, Frontier Institutes of Science and Technology, Xi’an Jiaotong UniversityCenter for Neuron and Disease, Frontier Institutes of Science and Technology, Xi’an Jiaotong UniversityCenter for Neuron and Disease, Frontier Institutes of Science and Technology, Xi’an Jiaotong UniversityCenter for Neuron and Disease, Frontier Institutes of Science and Technology, Xi’an Jiaotong UniversityDepartment of Physiology, University of TorontoCenter for Neuron and Disease, Frontier Institutes of Science and Technology, Xi’an Jiaotong UniversityCenter for Neuron and Disease, Frontier Institutes of Science and Technology, Xi’an Jiaotong UniversityCenter for Neuron and Disease, Frontier Institutes of Science and Technology, Xi’an Jiaotong UniversityCenter for Neuron and Disease, Frontier Institutes of Science and Technology, Xi’an Jiaotong UniversityAbstract Introduction N-Methyl-D-aspartate receptors (NMDARs) play a critical role in different forms of plasticity in the central nervous system. NMDARs are always assembled in tetrameric form, in which two GluN1 subunits and two GluN2 and/or GluN3 subunits combine together. Previous studies focused mainly on the hippocampus. The anterior cingulate cortex (ACC) is a key cortical region for sensory and emotional functions. NMDAR GluN2A and GluN2B subunits have been previously investigated, however much less is known about the GluN2C/2D subunits. Results In the present study, we found that the GluN2C/2D subunits are expressed in the pyramidal cells of ACC of adult mice. Application of a selective antagonist of GluN2C/2D, (2R*,3S*)-1-(9-bromophenanthrene-3-carbonyl) piperazine-2,3-dicarboxylic acid (UBP145), significantly reduced NMDAR-mediated currents, while synaptically evoked EPSCs were not affected. UBP145 affected neither the postsynaptic long-term potentiation (post-LTP) nor the presynaptic LTP (pre-LTP). Furthermore, the long-term depression (LTD) was also not affected by UBP145. Finally, both UBP145 decreased the frequency of the miniature EPSCs (mEPSCs) while the amplitude remained intact, suggesting that the GluN2C/2D may be involved in presynaptic regulation of spontaneous glutamate release. Conclusions Our results provide direct evidence that the GluN2C/2D contributes to evoked NMDAR mediated currents and mEPSCs in the ACC, which may have significant physiological implications.https://doi.org/10.1186/s13041-021-00744-3NMDARGluN2C/2DACCLTPLTD |
| spellingShingle | Qi-Yu Chen Xu-Hui Li Jing-Shan Lu Yinglu Liu Jung-Hyun Alex Lee Yu-Xin Chen Wantong Shi Kexin Fan Min Zhuo NMDA GluN2C/2D receptors contribute to synaptic regulation and plasticity in the anterior cingulate cortex of adult mice Molecular Brain NMDAR GluN2C/2D ACC LTP LTD |
| title | NMDA GluN2C/2D receptors contribute to synaptic regulation and plasticity in the anterior cingulate cortex of adult mice |
| title_full | NMDA GluN2C/2D receptors contribute to synaptic regulation and plasticity in the anterior cingulate cortex of adult mice |
| title_fullStr | NMDA GluN2C/2D receptors contribute to synaptic regulation and plasticity in the anterior cingulate cortex of adult mice |
| title_full_unstemmed | NMDA GluN2C/2D receptors contribute to synaptic regulation and plasticity in the anterior cingulate cortex of adult mice |
| title_short | NMDA GluN2C/2D receptors contribute to synaptic regulation and plasticity in the anterior cingulate cortex of adult mice |
| title_sort | nmda glun2c 2d receptors contribute to synaptic regulation and plasticity in the anterior cingulate cortex of adult mice |
| topic | NMDAR GluN2C/2D ACC LTP LTD |
| url | https://doi.org/10.1186/s13041-021-00744-3 |
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