Molecular Detection of Porcine Parainfluenza Viruses 1 and 5 Using a Newly Developed Duplex Real-Time RT-PCR in South Korea
Two species of porcine parainfluenza viruses (PPIV), PPIV1 and PPIV5, are globally distributed in pig herds and associated with porcine respiratory diseases, and a diagnostic tool for the simultaneous detection of the two viruses is required. In this study, a TaqMan probe-based duplex real-time reve...
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MDPI AG
2023-02-01
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| author | Jong-Min Kim Hye-Ryung Kim Gyu-Tae Jeon Ji-Su Baek Oh-Deog Kwon Choi-Kyu Park |
| author_facet | Jong-Min Kim Hye-Ryung Kim Gyu-Tae Jeon Ji-Su Baek Oh-Deog Kwon Choi-Kyu Park |
| author_sort | Jong-Min Kim |
| collection | DOAJ |
| description | Two species of porcine parainfluenza viruses (PPIV), PPIV1 and PPIV5, are globally distributed in pig herds and associated with porcine respiratory diseases, and a diagnostic tool for the simultaneous detection of the two viruses is required. In this study, a TaqMan probe-based duplex real-time reverse transcription polymerase chain reaction (dqRT-PCR) assay was first developed for the differential detection of PPIV1 and PPIV5 nucleocapsid protein (NP) genes in porcine clinical samples. The dqRT-PCR assay was highly sensitive, its limit of detection was approximately 10 RNA copies/reaction, it specifically amplified the targeted NP genes of PPIV1 and PPIV5 without cross-reacting with other porcine pathogens, and their clinical detection rates were 15.2% and 0.7%, respectively. The results from 441 clinical samples taken from 278 Korean domestic pig farms showed that the prevalence of PPIV1 and PPIV5 was 11.2% and 1.1%, respectively, and co-infection of both viruses was confirmed in a farm, suggesting that PPIV1 and PPIV5 are co-circulating in current Korean pig herds. Phylogenetic analysis based on the partial NP genes suggested that genetically diverse PPIV1 strains are circulating in Korean pig herds. The developed dqRT-PCR assay was found to be an accurate, reliable, and quantitative detection tool for PPIV1 and PPIV5 RNA in clinical pig samples and will be useful for etiological and epidemiological studies and the control of viral infections in the field. |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T09:15:55Z |
| publishDate | 2023-02-01 |
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| series | Animals |
| spelling | doaj.art-4f1762b8aacd45c290c584023aec24562023-11-16T18:38:50ZengMDPI AGAnimals2076-26152023-02-0113459810.3390/ani13040598Molecular Detection of Porcine Parainfluenza Viruses 1 and 5 Using a Newly Developed Duplex Real-Time RT-PCR in South KoreaJong-Min Kim0Hye-Ryung Kim1Gyu-Tae Jeon2Ji-Su Baek3Oh-Deog Kwon4Choi-Kyu Park5Animal Disease Intervention Center, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaAnimal Disease Intervention Center, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaAnimal Disease Intervention Center, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaAnimal Disease Intervention Center, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaAnimal Disease Intervention Center, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaAnimal Disease Intervention Center, College of Veterinary Medicine, Kyungpook National University, Daegu 41566, Republic of KoreaTwo species of porcine parainfluenza viruses (PPIV), PPIV1 and PPIV5, are globally distributed in pig herds and associated with porcine respiratory diseases, and a diagnostic tool for the simultaneous detection of the two viruses is required. In this study, a TaqMan probe-based duplex real-time reverse transcription polymerase chain reaction (dqRT-PCR) assay was first developed for the differential detection of PPIV1 and PPIV5 nucleocapsid protein (NP) genes in porcine clinical samples. The dqRT-PCR assay was highly sensitive, its limit of detection was approximately 10 RNA copies/reaction, it specifically amplified the targeted NP genes of PPIV1 and PPIV5 without cross-reacting with other porcine pathogens, and their clinical detection rates were 15.2% and 0.7%, respectively. The results from 441 clinical samples taken from 278 Korean domestic pig farms showed that the prevalence of PPIV1 and PPIV5 was 11.2% and 1.1%, respectively, and co-infection of both viruses was confirmed in a farm, suggesting that PPIV1 and PPIV5 are co-circulating in current Korean pig herds. Phylogenetic analysis based on the partial NP genes suggested that genetically diverse PPIV1 strains are circulating in Korean pig herds. The developed dqRT-PCR assay was found to be an accurate, reliable, and quantitative detection tool for PPIV1 and PPIV5 RNA in clinical pig samples and will be useful for etiological and epidemiological studies and the control of viral infections in the field.https://www.mdpi.com/2076-2615/13/4/598porcine parainfluenza virus 1porcine parainfluenza virus 5dqRT-PCR |
| spellingShingle | Jong-Min Kim Hye-Ryung Kim Gyu-Tae Jeon Ji-Su Baek Oh-Deog Kwon Choi-Kyu Park Molecular Detection of Porcine Parainfluenza Viruses 1 and 5 Using a Newly Developed Duplex Real-Time RT-PCR in South Korea Animals porcine parainfluenza virus 1 porcine parainfluenza virus 5 dqRT-PCR |
| title | Molecular Detection of Porcine Parainfluenza Viruses 1 and 5 Using a Newly Developed Duplex Real-Time RT-PCR in South Korea |
| title_full | Molecular Detection of Porcine Parainfluenza Viruses 1 and 5 Using a Newly Developed Duplex Real-Time RT-PCR in South Korea |
| title_fullStr | Molecular Detection of Porcine Parainfluenza Viruses 1 and 5 Using a Newly Developed Duplex Real-Time RT-PCR in South Korea |
| title_full_unstemmed | Molecular Detection of Porcine Parainfluenza Viruses 1 and 5 Using a Newly Developed Duplex Real-Time RT-PCR in South Korea |
| title_short | Molecular Detection of Porcine Parainfluenza Viruses 1 and 5 Using a Newly Developed Duplex Real-Time RT-PCR in South Korea |
| title_sort | molecular detection of porcine parainfluenza viruses 1 and 5 using a newly developed duplex real time rt pcr in south korea |
| topic | porcine parainfluenza virus 1 porcine parainfluenza virus 5 dqRT-PCR |
| url | https://www.mdpi.com/2076-2615/13/4/598 |
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