Relationship between Phenolic Compounds and Antioxidant Activity in Berries and Leaves of Raspberry Genotypes and Their Genotyping by SSR Markers
The red raspberry is one of the world’s most popular berries. The main direction of its breeding has switched to nutritional quality, and the evaluation of raspberry germplasm for antioxidant content and activity is very important. As berries, raspberry leaves contain valuable bioactive compounds, b...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/10/1961 |
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| author | Vadim G. Lebedev Tatyana N. Lebedeva Elena O. Vidyagina Vladimir N. Sorokopudov Anna A. Popova Konstantin A. Shestibratov |
| author_facet | Vadim G. Lebedev Tatyana N. Lebedeva Elena O. Vidyagina Vladimir N. Sorokopudov Anna A. Popova Konstantin A. Shestibratov |
| author_sort | Vadim G. Lebedev |
| collection | DOAJ |
| description | The red raspberry is one of the world’s most popular berries. The main direction of its breeding has switched to nutritional quality, and the evaluation of raspberry germplasm for antioxidant content and activity is very important. As berries, raspberry leaves contain valuable bioactive compounds, but the optimal time for their collection is unknown. We evaluated 25 new breeding lines and standard raspberry cultivars for their polyphenolic content and antioxidant capacity. The antioxidant activity of berries correlated better with the content of total phenolics (0.88 and 0.92) and flavonoids (0.76 and 0.88) than with anthocyanins (0.37 and 0.66). Two breeding lines were significantly superior to the standard cultivars and can be used in further breeding. Leaves collected in three phenological phases of the raspberry contained more phenolics (5.4-fold) and flavonoids (4.1-fold) and showed higher antioxidant activities (2.4-fold in FRAP assay, 2.2-fold in ABTS) than berries. The optimal time for harvesting raspberry leaves is the fruit ripening stage, with exceptions for some cultivars. Genetic diversity analysis using microsatellite (SSR) markers from flavonoid biosynthesis genes divided the genotypes into five clusters, generally in agreement with their kinships. The relationship between genetic data based on metabolism-specific SSR markers and the chemical diversity of cultivars was first assessed. The biochemical and genetic results show a strong correlation (0.78). This study is useful for further the improvement of raspberry and other berry crops. |
| first_indexed | 2024-03-09T20:48:32Z |
| format | Article |
| id | doaj.art-4f1826234ae443b89b5dea8200a02ca2 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T20:48:32Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-4f1826234ae443b89b5dea8200a02ca22023-11-23T22:38:37ZengMDPI AGAntioxidants2076-39212022-09-011110196110.3390/antiox11101961Relationship between Phenolic Compounds and Antioxidant Activity in Berries and Leaves of Raspberry Genotypes and Their Genotyping by SSR MarkersVadim G. Lebedev0Tatyana N. Lebedeva1Elena O. Vidyagina2Vladimir N. Sorokopudov3Anna A. Popova4Konstantin A. Shestibratov5Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Prospekt Nauki 6, 142290 Pushchino, RussiaInstitute of Physicochemical and Biological Problems in Soil Science of the Russian Academy of Sciences, Instituskaya Str. 2, 142290 Pushchino, RussiaBranch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Prospekt Nauki 6, 142290 Pushchino, RussiaAll-Russian Scientific Research Institute of Medicinal and Aromatic Plants, Grina Str. 7, 117216 Moscow, RussiaDepartment of Botany and Plant Physiology, Voronezh State University of Forestry and Technologies Named after G.F. Morozov, Timiryazeva Str. 8, 394087 Voronezh, RussiaBranch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Prospekt Nauki 6, 142290 Pushchino, RussiaThe red raspberry is one of the world’s most popular berries. The main direction of its breeding has switched to nutritional quality, and the evaluation of raspberry germplasm for antioxidant content and activity is very important. As berries, raspberry leaves contain valuable bioactive compounds, but the optimal time for their collection is unknown. We evaluated 25 new breeding lines and standard raspberry cultivars for their polyphenolic content and antioxidant capacity. The antioxidant activity of berries correlated better with the content of total phenolics (0.88 and 0.92) and flavonoids (0.76 and 0.88) than with anthocyanins (0.37 and 0.66). Two breeding lines were significantly superior to the standard cultivars and can be used in further breeding. Leaves collected in three phenological phases of the raspberry contained more phenolics (5.4-fold) and flavonoids (4.1-fold) and showed higher antioxidant activities (2.4-fold in FRAP assay, 2.2-fold in ABTS) than berries. The optimal time for harvesting raspberry leaves is the fruit ripening stage, with exceptions for some cultivars. Genetic diversity analysis using microsatellite (SSR) markers from flavonoid biosynthesis genes divided the genotypes into five clusters, generally in agreement with their kinships. The relationship between genetic data based on metabolism-specific SSR markers and the chemical diversity of cultivars was first assessed. The biochemical and genetic results show a strong correlation (0.78). This study is useful for further the improvement of raspberry and other berry crops.https://www.mdpi.com/2076-3921/11/10/1961antioxidantsanthocyaninsflavonoid biosynthesisgenetic diversityphenological phase<i>Rubus idaeus</i> |
| spellingShingle | Vadim G. Lebedev Tatyana N. Lebedeva Elena O. Vidyagina Vladimir N. Sorokopudov Anna A. Popova Konstantin A. Shestibratov Relationship between Phenolic Compounds and Antioxidant Activity in Berries and Leaves of Raspberry Genotypes and Their Genotyping by SSR Markers Antioxidants antioxidants anthocyanins flavonoid biosynthesis genetic diversity phenological phase <i>Rubus idaeus</i> |
| title | Relationship between Phenolic Compounds and Antioxidant Activity in Berries and Leaves of Raspberry Genotypes and Their Genotyping by SSR Markers |
| title_full | Relationship between Phenolic Compounds and Antioxidant Activity in Berries and Leaves of Raspberry Genotypes and Their Genotyping by SSR Markers |
| title_fullStr | Relationship between Phenolic Compounds and Antioxidant Activity in Berries and Leaves of Raspberry Genotypes and Their Genotyping by SSR Markers |
| title_full_unstemmed | Relationship between Phenolic Compounds and Antioxidant Activity in Berries and Leaves of Raspberry Genotypes and Their Genotyping by SSR Markers |
| title_short | Relationship between Phenolic Compounds and Antioxidant Activity in Berries and Leaves of Raspberry Genotypes and Their Genotyping by SSR Markers |
| title_sort | relationship between phenolic compounds and antioxidant activity in berries and leaves of raspberry genotypes and their genotyping by ssr markers |
| topic | antioxidants anthocyanins flavonoid biosynthesis genetic diversity phenological phase <i>Rubus idaeus</i> |
| url | https://www.mdpi.com/2076-3921/11/10/1961 |
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