Altered immunity in crowded Mythimna separata is mediated by octopamine and dopamine
Abstract Similar to pathogenic infection, high population density alters insects’ prophylactic immunity. Density-dependent prophylaxis has been reported in many polyphenic insects, but the regulatory mechanism underlying this phenomenon remains unclear. The biogenic monoamines are known to play crit...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2018-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-20711-8 |
| _version_ | 1818998105645776896 |
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| author | Hailong Kong Chuanlei Dong Zhen Tian Nian Mao Cheng Wang Yunxia Cheng Lei Zhang Xingfu Jiang Lizhi Luo |
| author_facet | Hailong Kong Chuanlei Dong Zhen Tian Nian Mao Cheng Wang Yunxia Cheng Lei Zhang Xingfu Jiang Lizhi Luo |
| author_sort | Hailong Kong |
| collection | DOAJ |
| description | Abstract Similar to pathogenic infection, high population density alters insects’ prophylactic immunity. Density-dependent prophylaxis has been reported in many polyphenic insects, but the regulatory mechanism underlying this phenomenon remains unclear. The biogenic monoamines are known to play critical roles in mediating insect immune responses. In the current study, the immune capacity and the levels of three biogenic monoamines were investigated in the polyphenic larvae of Mythimna separata, reared at the densities of 1, 2, 5, 10, and 30 larvae per 650-mL jar. Concomitant with the increased phenoloxidase (PO) activity and total haemocyte count in the larvae at high densities (5, 10, 30 larvae/jar), the octopamine level was also increased. In contrast, the dopamine level was decreased, and the 5-hydroxytryptamine level was not significantly affected. Injection of octopamine induced significant increases in the total haemocyte count and PO activity. Conversely, epinastine, a specific antagonist of octopamine, decreased the total haemocyte count and PO activity. Another octopamine antagonist, phentolamine, inhibited the activity of PO and lysozymes. In addition, injection of dopamine induced a significant increase in PO activity and decreased the total haemocyte count and lysozyme activity. These results suggested that both octopamine and dopamine mediate the increases in total haemocyte count and PO activity in the crowded larvae. |
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| format | Article |
| id | doaj.art-4f191e87bee3452e9dc5740c79612ac3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:56:14Z |
| publishDate | 2018-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4f191e87bee3452e9dc5740c79612ac32022-12-21T19:25:27ZengNature PortfolioScientific Reports2045-23222018-02-018111010.1038/s41598-018-20711-8Altered immunity in crowded Mythimna separata is mediated by octopamine and dopamineHailong Kong0Chuanlei Dong1Zhen Tian2Nian Mao3Cheng Wang4Yunxia Cheng5Lei Zhang6Xingfu Jiang7Lizhi Luo8College of Horticulture and Plant Protection, Yangzhou UniversityCollege of Horticulture and Plant Protection, Yangzhou UniversityCollege of Horticulture and Plant Protection, Yangzhou UniversityCollege of Horticulture and Plant Protection, Yangzhou UniversityCollege of Horticulture and Plant Protection, Yangzhou UniversityState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural SciencesAbstract Similar to pathogenic infection, high population density alters insects’ prophylactic immunity. Density-dependent prophylaxis has been reported in many polyphenic insects, but the regulatory mechanism underlying this phenomenon remains unclear. The biogenic monoamines are known to play critical roles in mediating insect immune responses. In the current study, the immune capacity and the levels of three biogenic monoamines were investigated in the polyphenic larvae of Mythimna separata, reared at the densities of 1, 2, 5, 10, and 30 larvae per 650-mL jar. Concomitant with the increased phenoloxidase (PO) activity and total haemocyte count in the larvae at high densities (5, 10, 30 larvae/jar), the octopamine level was also increased. In contrast, the dopamine level was decreased, and the 5-hydroxytryptamine level was not significantly affected. Injection of octopamine induced significant increases in the total haemocyte count and PO activity. Conversely, epinastine, a specific antagonist of octopamine, decreased the total haemocyte count and PO activity. Another octopamine antagonist, phentolamine, inhibited the activity of PO and lysozymes. In addition, injection of dopamine induced a significant increase in PO activity and decreased the total haemocyte count and lysozyme activity. These results suggested that both octopamine and dopamine mediate the increases in total haemocyte count and PO activity in the crowded larvae.https://doi.org/10.1038/s41598-018-20711-8 |
| spellingShingle | Hailong Kong Chuanlei Dong Zhen Tian Nian Mao Cheng Wang Yunxia Cheng Lei Zhang Xingfu Jiang Lizhi Luo Altered immunity in crowded Mythimna separata is mediated by octopamine and dopamine Scientific Reports |
| title | Altered immunity in crowded Mythimna separata is mediated by octopamine and dopamine |
| title_full | Altered immunity in crowded Mythimna separata is mediated by octopamine and dopamine |
| title_fullStr | Altered immunity in crowded Mythimna separata is mediated by octopamine and dopamine |
| title_full_unstemmed | Altered immunity in crowded Mythimna separata is mediated by octopamine and dopamine |
| title_short | Altered immunity in crowded Mythimna separata is mediated by octopamine and dopamine |
| title_sort | altered immunity in crowded mythimna separata is mediated by octopamine and dopamine |
| url | https://doi.org/10.1038/s41598-018-20711-8 |
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