The Effect of Triclabendazole on ALT Enzyme activity in Fasciola hepatica helminths and parasitized sheep liver tissues
Introduction: To determine an indicator for Triclabendazole (TCBZ) efficacy, Alanine aminotransferase (ALT) activity in Fasciola hepatica (Iranian isolate) parasite in presence and absence of TCBZ was evaluated by an in vitro cultivation method. Also, ALT enzyme activity between none and parasitized...
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| Format: | Article |
| Language: | English |
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Pasteur Institute of Iran
2015-01-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
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| Online Access: | http://jommid.pasteur.ac.ir/article-1-81-en.html |
| _version_ | 1819170428988424192 |
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| author | Ali Farahnak Fariba Amni Taghi Golmohammadi Mohammad Reza Eshraghian Mohammad Bagher Molaei Rad |
| author_facet | Ali Farahnak Fariba Amni Taghi Golmohammadi Mohammad Reza Eshraghian Mohammad Bagher Molaei Rad |
| author_sort | Ali Farahnak |
| collection | DOAJ |
| description | Introduction: To determine an indicator for Triclabendazole (TCBZ) efficacy, Alanine aminotransferase (ALT) activity in Fasciola hepatica (Iranian isolate) parasite in presence and absence of TCBZ was evaluated by an in vitro cultivation method. Also, ALT enzyme activity between none and parasitized-infected sheep liver tissues was assessed. Method: The sheep livers were collected and transferred immediately to the Department of Parasitology. Adult living parasites were recovered, washed and divided into two groups, treatment and control groups with 10 parasites in each. We added 15 μg TCBZ to the treatment group; then incubated both groups for 4 h at 37ºC. The parasites, infected and parasite free liver tissues were ground and homogenized by a Mortar and pestle, centrifuged, and supernatants were recovered. Protein concentration and ALT enzyme activity were measured in the supenatants. Results: The results of ALT enzyme activity assay showed 0.03 U/ml/mg protein for treated F. hepatica and 0.01 U/ml/mg protein for untreated samples, the mean values of difference was not significant (p>0.05). The difference between ALT activity in none and parasitized-infected liver was not significant (p>0.05). However, two-sample T-test analysis showed higher ALT activity in treated and untreated parasite in comparison with none and parasitized-infected liver specimens (p<0.05). In addition to ALT protein band for parasite and liver tissue, Cathepsin enzyme (proteases) was detected for parasite by SDS-PAGE analysis. Conclusion: ALT activity cannot be considered as a useful marker for TCBZ efficacy in F. hepatica treatment. However, ALT enzyme showed comparable activities in parasite and its host liver tissue. |
| first_indexed | 2024-12-22T19:35:15Z |
| format | Article |
| id | doaj.art-4f213e01f61e4e3f97d7e8a290217146 |
| institution | Directory Open Access Journal |
| issn | 2345-5349 2345-5330 |
| language | English |
| last_indexed | 2024-12-22T19:35:15Z |
| publishDate | 2015-01-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Journal of Medical Microbiology and Infectious Diseases |
| spelling | doaj.art-4f213e01f61e4e3f97d7e8a2902171462022-12-21T18:14:59ZengPasteur Institute of IranJournal of Medical Microbiology and Infectious Diseases2345-53492345-53302015-01-013115The Effect of Triclabendazole on ALT Enzyme activity in Fasciola hepatica helminths and parasitized sheep liver tissuesAli Farahnak0Fariba Amni1Taghi Golmohammadi2Mohammad Reza Eshraghian3Mohammad Bagher Molaei Rad4 Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Biochemistry, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran Introduction: To determine an indicator for Triclabendazole (TCBZ) efficacy, Alanine aminotransferase (ALT) activity in Fasciola hepatica (Iranian isolate) parasite in presence and absence of TCBZ was evaluated by an in vitro cultivation method. Also, ALT enzyme activity between none and parasitized-infected sheep liver tissues was assessed. Method: The sheep livers were collected and transferred immediately to the Department of Parasitology. Adult living parasites were recovered, washed and divided into two groups, treatment and control groups with 10 parasites in each. We added 15 μg TCBZ to the treatment group; then incubated both groups for 4 h at 37ºC. The parasites, infected and parasite free liver tissues were ground and homogenized by a Mortar and pestle, centrifuged, and supernatants were recovered. Protein concentration and ALT enzyme activity were measured in the supenatants. Results: The results of ALT enzyme activity assay showed 0.03 U/ml/mg protein for treated F. hepatica and 0.01 U/ml/mg protein for untreated samples, the mean values of difference was not significant (p>0.05). The difference between ALT activity in none and parasitized-infected liver was not significant (p>0.05). However, two-sample T-test analysis showed higher ALT activity in treated and untreated parasite in comparison with none and parasitized-infected liver specimens (p<0.05). In addition to ALT protein band for parasite and liver tissue, Cathepsin enzyme (proteases) was detected for parasite by SDS-PAGE analysis. Conclusion: ALT activity cannot be considered as a useful marker for TCBZ efficacy in F. hepatica treatment. However, ALT enzyme showed comparable activities in parasite and its host liver tissue.http://jommid.pasteur.ac.ir/article-1-81-en.htmlalanine aminotransferasefasciola hepaticaegaten®triclabendazoleliver |
| spellingShingle | Ali Farahnak Fariba Amni Taghi Golmohammadi Mohammad Reza Eshraghian Mohammad Bagher Molaei Rad The Effect of Triclabendazole on ALT Enzyme activity in Fasciola hepatica helminths and parasitized sheep liver tissues Journal of Medical Microbiology and Infectious Diseases alanine aminotransferase fasciola hepatica egaten® triclabendazole liver |
| title | The Effect of Triclabendazole on ALT Enzyme activity in Fasciola hepatica helminths and parasitized sheep liver tissues |
| title_full | The Effect of Triclabendazole on ALT Enzyme activity in Fasciola hepatica helminths and parasitized sheep liver tissues |
| title_fullStr | The Effect of Triclabendazole on ALT Enzyme activity in Fasciola hepatica helminths and parasitized sheep liver tissues |
| title_full_unstemmed | The Effect of Triclabendazole on ALT Enzyme activity in Fasciola hepatica helminths and parasitized sheep liver tissues |
| title_short | The Effect of Triclabendazole on ALT Enzyme activity in Fasciola hepatica helminths and parasitized sheep liver tissues |
| title_sort | effect of triclabendazole on alt enzyme activity in fasciola hepatica helminths and parasitized sheep liver tissues |
| topic | alanine aminotransferase fasciola hepatica egaten® triclabendazole liver |
| url | http://jommid.pasteur.ac.ir/article-1-81-en.html |
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