Bioaminergic Responses in an In Vitro System Studying Human Gut Microbiota–Kiwifruit Interactions
Whole kiwifruit (‘Hayward’ and ‘Zesy002’) were examined for their bioaminergic potential after being subjected to in vitro gastrointestinal digestion and colonic fermentation. Controls included the prebiotic inulin and water, a carbohydrate-free vehicle. The dopamine precursor <span style="f...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/10/1582 |
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| author | Shanthi G. Parkar Carel M. H. Jobsis Tania M. Trower Janine M. Cooney Duncan I. Hedderley Kerry L. Bentley-Hewitt |
| author_facet | Shanthi G. Parkar Carel M. H. Jobsis Tania M. Trower Janine M. Cooney Duncan I. Hedderley Kerry L. Bentley-Hewitt |
| author_sort | Shanthi G. Parkar |
| collection | DOAJ |
| description | Whole kiwifruit (‘Hayward’ and ‘Zesy002’) were examined for their bioaminergic potential after being subjected to in vitro gastrointestinal digestion and colonic fermentation. Controls included the prebiotic inulin and water, a carbohydrate-free vehicle. The dopamine precursor <span style="font-variant: small-caps;">l</span>-dihydroxyphenylalanine (L-DOPA) and the serotonin precursor 5-hydroxytryptophan were increased in the kiwifruit gastrointestinal digesta (‘Hayward’ > ‘Zesy002’) in comparison to the water digesta. Fermentation of the digesta with human fecal bacteria for 18 h modulated the concentrations of bioamine metabolites. The most notable were the significant increases in L-DOPA (‘Zesy002’ > ‘Hayward’) and γ-aminobutyric acid (GABA) (‘Hayward’ > ‘Zesy002’). Kiwifruit increased <i>Bifidobacterium</i> spp. and Veillonellaceae (correlating with L-DOPA increase), and <i>Lachnospira</i> spp. (correlating with GABA). The digesta and fermenta were incubated with Caco-2 cells for 3 h followed by gene expression analysis. Effects were seen on genes related to serotonin synthesis/re-uptake/conversion to melatonin, gut tight junction, inflammation and circadian rhythm with different digesta and fermenta from the four treatments. These indicate potential effects of the substrates and the microbially generated organic acid and bioamine metabolites on intestinal functions that have physiological relevance. Further studies are required to confirm the potential bioaminergic effects of gut microbiota–kiwifruit interactions. |
| first_indexed | 2024-03-10T15:38:25Z |
| format | Article |
| id | doaj.art-4f215a8fac6a460cb0e40585a78ed73f |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T15:38:25Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-4f215a8fac6a460cb0e40585a78ed73f2023-11-20T17:04:33ZengMDPI AGMicroorganisms2076-26072020-10-01810158210.3390/microorganisms8101582Bioaminergic Responses in an In Vitro System Studying Human Gut Microbiota–Kiwifruit InteractionsShanthi G. Parkar0Carel M. H. Jobsis1Tania M. Trower2Janine M. Cooney3Duncan I. Hedderley4Kerry L. Bentley-Hewitt5The New Zealand Institute for Plant and Food Research Limited (Plant & Food Research), Private Bag 11600, Palmerston North 4442, New ZealandThe New Zealand Institute for Plant and Food Research Limited (Plant & Food Research), Private Bag 11600, Palmerston North 4442, New ZealandPlant & Food Research, Private Bag 11600, Hamilton 3240, New ZealandPlant & Food Research, Private Bag 11600, Hamilton 3240, New ZealandThe New Zealand Institute for Plant and Food Research Limited (Plant & Food Research), Private Bag 11600, Palmerston North 4442, New ZealandThe New Zealand Institute for Plant and Food Research Limited (Plant & Food Research), Private Bag 11600, Palmerston North 4442, New ZealandWhole kiwifruit (‘Hayward’ and ‘Zesy002’) were examined for their bioaminergic potential after being subjected to in vitro gastrointestinal digestion and colonic fermentation. Controls included the prebiotic inulin and water, a carbohydrate-free vehicle. The dopamine precursor <span style="font-variant: small-caps;">l</span>-dihydroxyphenylalanine (L-DOPA) and the serotonin precursor 5-hydroxytryptophan were increased in the kiwifruit gastrointestinal digesta (‘Hayward’ > ‘Zesy002’) in comparison to the water digesta. Fermentation of the digesta with human fecal bacteria for 18 h modulated the concentrations of bioamine metabolites. The most notable were the significant increases in L-DOPA (‘Zesy002’ > ‘Hayward’) and γ-aminobutyric acid (GABA) (‘Hayward’ > ‘Zesy002’). Kiwifruit increased <i>Bifidobacterium</i> spp. and Veillonellaceae (correlating with L-DOPA increase), and <i>Lachnospira</i> spp. (correlating with GABA). The digesta and fermenta were incubated with Caco-2 cells for 3 h followed by gene expression analysis. Effects were seen on genes related to serotonin synthesis/re-uptake/conversion to melatonin, gut tight junction, inflammation and circadian rhythm with different digesta and fermenta from the four treatments. These indicate potential effects of the substrates and the microbially generated organic acid and bioamine metabolites on intestinal functions that have physiological relevance. Further studies are required to confirm the potential bioaminergic effects of gut microbiota–kiwifruit interactions.https://www.mdpi.com/2076-2607/8/10/1582green-fleshed kiwifruitZespri<sup>®</sup> Green kiwifruitgold-fleshed kiwifruitGold3 kiwifruitZespri<sup>®</sup> SunGold kiwifruitbiogenic amines (BAs) |
| spellingShingle | Shanthi G. Parkar Carel M. H. Jobsis Tania M. Trower Janine M. Cooney Duncan I. Hedderley Kerry L. Bentley-Hewitt Bioaminergic Responses in an In Vitro System Studying Human Gut Microbiota–Kiwifruit Interactions Microorganisms green-fleshed kiwifruit Zespri<sup>®</sup> Green kiwifruit gold-fleshed kiwifruit Gold3 kiwifruit Zespri<sup>®</sup> SunGold kiwifruit biogenic amines (BAs) |
| title | Bioaminergic Responses in an In Vitro System Studying Human Gut Microbiota–Kiwifruit Interactions |
| title_full | Bioaminergic Responses in an In Vitro System Studying Human Gut Microbiota–Kiwifruit Interactions |
| title_fullStr | Bioaminergic Responses in an In Vitro System Studying Human Gut Microbiota–Kiwifruit Interactions |
| title_full_unstemmed | Bioaminergic Responses in an In Vitro System Studying Human Gut Microbiota–Kiwifruit Interactions |
| title_short | Bioaminergic Responses in an In Vitro System Studying Human Gut Microbiota–Kiwifruit Interactions |
| title_sort | bioaminergic responses in an in vitro system studying human gut microbiota kiwifruit interactions |
| topic | green-fleshed kiwifruit Zespri<sup>®</sup> Green kiwifruit gold-fleshed kiwifruit Gold3 kiwifruit Zespri<sup>®</sup> SunGold kiwifruit biogenic amines (BAs) |
| url | https://www.mdpi.com/2076-2607/8/10/1582 |
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