The Effects of Manual Acupuncture on Mitochondrial Fusion and Fission Gene Expression in Rat Spleen
Background : A significant amount of research has been conducted to establish the validity of acupuncture, and it has been demonstrated through animal disease model studies that acupuncture influences mitochondrial changes. However, to more accurately examine the mechanisms of acupuncture treatment...
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| Format: | Article |
| Language: | English |
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Medical Association of Pharmacopuncture Institute
2023-04-01
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| Series: | Journal of Acupuncture & Meridian Studies |
| Subjects: | |
| Online Access: | http://www.journal-jams.org/journal/view.html?doi=10.51507/j.jams.2023.16.2.49 |
| _version_ | 1797844041105145856 |
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| author | Yu-Mi Lee Dong-Hee Choi Jeong-Hye Park Min-Woo Cheon Jae Gwan Kim Jeong-Sang Kim Taejin Choi Hye-Ran Kim Daehwan Youn |
| author_facet | Yu-Mi Lee Dong-Hee Choi Jeong-Hye Park Min-Woo Cheon Jae Gwan Kim Jeong-Sang Kim Taejin Choi Hye-Ran Kim Daehwan Youn |
| author_sort | Yu-Mi Lee |
| collection | DOAJ |
| description | Background : A significant amount of research has been conducted to establish the validity of acupuncture, and it has been demonstrated through animal disease model studies that acupuncture influences mitochondrial changes. However, to more accurately examine the mechanisms of acupuncture treatment effectiveness in pathological models, it is crucial to investigate changes in disease-free animals. Among various hypotheses regarding the effects of acupuncture on the body, we focused on the result that acupuncture stimulation is related to mitochondria. Objectives: We examined the effects of acupuncture mitochondrial fission and fusionrelated mediators in disease-free Sprague Dawley (SD) rats' spleen meridian acupoints. Methods : SD rats were divided into control, SP1, SP2, SP3, SP5, and SP9 acupuncture groups. Acupuncture was performed at each point for 10 minutes daily for four days. Peroxisome proliferator-activated receptor-gamma coactivator 1-α (PGC-1α) and fission protein 1 (Fis1) levels were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR), while dynamin-related protein 1 (DRP1), optic atrophy-1 (OPA1), mitofusin-1 (MFN1), and mitofusin-2 (MFN2) levels were assessed via western blotting. Mitochondria protein concentrations and NADH dehydrogenase activity in spleen tissues were measured using enzyme-linked immunosorbent assay (ELISA). Results : PGC-1α expression decreased in the SP1 (p < 0.01), SP5 (p < 0.05), and SP9 (p < 0.05) groups, while Fis1 expression increased in the SP1 (p < 0.01), SP5 (p < 0.01), and SP9 (p < 0.05) groups. DRP1, OPA1, MFN1, and MFN2 levels exhibited no significant changes. Mitochondrial protein concentrations decreased in the SP2 (p < 0.01), SP3 (p < 0.01), SP5 (p < 0.01), and SP9 (p < 0.01) groups, while NADH dehydrogenase activity decreased in the SP2 (p < 0.05) and SP9 (p < 0.05) groups. Conclusion : Acupuncture at the SP9 acupoint influenced the mitochondrial fission pathway by modulating PGC-1α and Fis1 mediators in the rat spleen under non-disease conditions. |
| first_indexed | 2024-04-09T17:16:03Z |
| format | Article |
| id | doaj.art-4f23156369394f50b05af216c1ef8fd2 |
| institution | Directory Open Access Journal |
| issn | 2005-2901 |
| language | English |
| last_indexed | 2024-04-09T17:16:03Z |
| publishDate | 2023-04-01 |
| publisher | Medical Association of Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Acupuncture & Meridian Studies |
| spelling | doaj.art-4f23156369394f50b05af216c1ef8fd22023-04-20T00:41:51ZengMedical Association of Pharmacopuncture InstituteJournal of Acupuncture & Meridian Studies2005-29012023-04-01162495510.51507/j.jams.2023.16.2.49j.jams.2023.16.2.49The Effects of Manual Acupuncture on Mitochondrial Fusion and Fission Gene Expression in Rat SpleenYu-Mi Lee0Dong-Hee Choi1Jeong-Hye Park2Min-Woo Cheon3Jae Gwan Kim4Jeong-Sang Kim5Taejin Choi6Hye-Ran Kim7Daehwan Youn8Department of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, KoreaDepartment of Korean Medicine, Dongshin University, Naju, KoreaDepartment of Korean Medicine, Dongshin University, Naju, KoreaDepartment of Health Administration, Dongshin University, Naju, KoreaDepartment of Biomedical Science and Engineering, Gwangju Institute of Science and Technology, Gwangju, KoreaDepartment of Korean Medicine, Dongshin University, Naju, KoreaDongHaeng Convalescent Hospital, Gwangju, KoreaDepartment of Korean Medicine, Dongshin University, Naju, KoreaDepartment of Korean Medicine, Dongshin University, Naju, KoreaBackground : A significant amount of research has been conducted to establish the validity of acupuncture, and it has been demonstrated through animal disease model studies that acupuncture influences mitochondrial changes. However, to more accurately examine the mechanisms of acupuncture treatment effectiveness in pathological models, it is crucial to investigate changes in disease-free animals. Among various hypotheses regarding the effects of acupuncture on the body, we focused on the result that acupuncture stimulation is related to mitochondria. Objectives: We examined the effects of acupuncture mitochondrial fission and fusionrelated mediators in disease-free Sprague Dawley (SD) rats' spleen meridian acupoints. Methods : SD rats were divided into control, SP1, SP2, SP3, SP5, and SP9 acupuncture groups. Acupuncture was performed at each point for 10 minutes daily for four days. Peroxisome proliferator-activated receptor-gamma coactivator 1-α (PGC-1α) and fission protein 1 (Fis1) levels were evaluated using quantitative real-time polymerase chain reaction (qRT-PCR), while dynamin-related protein 1 (DRP1), optic atrophy-1 (OPA1), mitofusin-1 (MFN1), and mitofusin-2 (MFN2) levels were assessed via western blotting. Mitochondria protein concentrations and NADH dehydrogenase activity in spleen tissues were measured using enzyme-linked immunosorbent assay (ELISA). Results : PGC-1α expression decreased in the SP1 (p < 0.01), SP5 (p < 0.05), and SP9 (p < 0.05) groups, while Fis1 expression increased in the SP1 (p < 0.01), SP5 (p < 0.01), and SP9 (p < 0.05) groups. DRP1, OPA1, MFN1, and MFN2 levels exhibited no significant changes. Mitochondrial protein concentrations decreased in the SP2 (p < 0.01), SP3 (p < 0.01), SP5 (p < 0.01), and SP9 (p < 0.01) groups, while NADH dehydrogenase activity decreased in the SP2 (p < 0.05) and SP9 (p < 0.05) groups. Conclusion : Acupuncture at the SP9 acupoint influenced the mitochondrial fission pathway by modulating PGC-1α and Fis1 mediators in the rat spleen under non-disease conditions.http://www.journal-jams.org/journal/view.html?doi=10.51507/j.jams.2023.16.2.49acupuncturemitochondrial fissionspleenpgc-1αfis1nadh dehydrogenase |
| spellingShingle | Yu-Mi Lee Dong-Hee Choi Jeong-Hye Park Min-Woo Cheon Jae Gwan Kim Jeong-Sang Kim Taejin Choi Hye-Ran Kim Daehwan Youn The Effects of Manual Acupuncture on Mitochondrial Fusion and Fission Gene Expression in Rat Spleen Journal of Acupuncture & Meridian Studies acupuncture mitochondrial fission spleen pgc-1α fis1 nadh dehydrogenase |
| title | The Effects of Manual Acupuncture on Mitochondrial Fusion and Fission Gene Expression in Rat Spleen |
| title_full | The Effects of Manual Acupuncture on Mitochondrial Fusion and Fission Gene Expression in Rat Spleen |
| title_fullStr | The Effects of Manual Acupuncture on Mitochondrial Fusion and Fission Gene Expression in Rat Spleen |
| title_full_unstemmed | The Effects of Manual Acupuncture on Mitochondrial Fusion and Fission Gene Expression in Rat Spleen |
| title_short | The Effects of Manual Acupuncture on Mitochondrial Fusion and Fission Gene Expression in Rat Spleen |
| title_sort | effects of manual acupuncture on mitochondrial fusion and fission gene expression in rat spleen |
| topic | acupuncture mitochondrial fission spleen pgc-1α fis1 nadh dehydrogenase |
| url | http://www.journal-jams.org/journal/view.html?doi=10.51507/j.jams.2023.16.2.49 |
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