Myb Immunohistochemical Staining and Fluorescence in situ Hybridization in Salivary Rare Basaloid Lesions
Objective: Salivary rare basaloid lesions, including cribriform type basal cell adenoma (cBCA), BCA with incomplete capsule (iBCA), sialoblastoma (SB), and intercalated duct hyperplasia (IDH), could easily be misdiagnosed as adenoid cystic carcinoma (AdCC). We aim to identify an approach for differe...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fonc.2020.00870/full |
| _version_ | 1819147343962832896 |
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| author | Binbin Li Binbin Li Binbin Li Weiping Jie Weiping Jie Weiping Jie Huiying He |
| author_facet | Binbin Li Binbin Li Binbin Li Weiping Jie Weiping Jie Weiping Jie Huiying He |
| author_sort | Binbin Li |
| collection | DOAJ |
| description | Objective: Salivary rare basaloid lesions, including cribriform type basal cell adenoma (cBCA), BCA with incomplete capsule (iBCA), sialoblastoma (SB), and intercalated duct hyperplasia (IDH), could easily be misdiagnosed as adenoid cystic carcinoma (AdCC). We aim to identify an approach for differential diagnosis and to establish an optimal workflow concerning the diagnosis of these lesions.Material and methods: A panel of antibodies (MYB, β-catenin, CD117, SOX10, ki67, P63, calponin) and fluorescence in situ hybridization (FISH)-MYB were utilized to distinguish above salivary basaloid diseases from AdCC.Results: Histologically, the striking diagnostic features of cBCA, iBCA, SB, and IDH are composed of basaloid tumor cells, well-defined encapsulation, or lack of destructive invasion. Immunohistochemically, Myb immune-labeling could effectively make a distinction among cBCA, iBCA, SB, and IDH from AdCC, except in SB. cBCA and iBCA typically expressed β-catenin in the nuclei of tumor cells. There was no statistical significance in the ki67 index between SB and AdCC, but their indices were significantly higher than those of iBCA and IDH (p < 0.05, p < 0.05, respectively). P63 and calponin immune-expression were observed in the basaloid or myoepithelial cells. CD117 were observed positively in cBCA, iBCA, SB, and AdCC, except in IDH. SOX10 were observed positively in all cases. No cases had fusion of MYB and NFIB detectable by FISH, except in AdCC.Conclusion: Considering their sensitivity and specificity, FISH-Myb and an immunohistochemical panel of MYB/β-catenin/ki67 would be an optimal choice for the differential diagnosis of these basaloid lesions.Clinical relevance: Some salivary basaloid tumor or tumor-like lesions have overlapping features with AdCC. Through this present research, we suggested that the panel IHC of MYB, βcatenin, and ki67 combined with FISH-Myb should be an optimal choice for differential diagnosis among those lesions. |
| first_indexed | 2024-12-22T13:28:19Z |
| format | Article |
| id | doaj.art-4f237577e4e24f0c9629f3d4d5947504 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-22T13:28:19Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-4f237577e4e24f0c9629f3d4d59475042022-12-21T18:24:15ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-06-011010.3389/fonc.2020.00870498238Myb Immunohistochemical Staining and Fluorescence in situ Hybridization in Salivary Rare Basaloid LesionsBinbin Li0Binbin Li1Binbin Li2Weiping Jie3Weiping Jie4Weiping Jie5Huiying He6Department of Oral Pathology, Peking University School and Hospital of Stomatology, Beijing, ChinaResearch Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions, Chinese Academy of Medical Sciences, Beijing, ChinaNational Clinical Research Center for Oral Diseases, Peking University School and Hospital of Stomatology, Beijing, ChinaDepartment of Oral Pathology, Peking University School and Hospital of Stomatology, Beijing, ChinaResearch Unit of Precision Pathologic Diagnosis in Tumors of the Oral and Maxillofacial Regions, Chinese Academy of Medical Sciences, Beijing, ChinaNational Clinical Research Center for Oral Diseases, Peking University School and Hospital of Stomatology, Beijing, ChinaDepartment of Pathology, School of Basic Medical Sciences, Third Hospital, Peking University Health Science Center, Beijing, ChinaObjective: Salivary rare basaloid lesions, including cribriform type basal cell adenoma (cBCA), BCA with incomplete capsule (iBCA), sialoblastoma (SB), and intercalated duct hyperplasia (IDH), could easily be misdiagnosed as adenoid cystic carcinoma (AdCC). We aim to identify an approach for differential diagnosis and to establish an optimal workflow concerning the diagnosis of these lesions.Material and methods: A panel of antibodies (MYB, β-catenin, CD117, SOX10, ki67, P63, calponin) and fluorescence in situ hybridization (FISH)-MYB were utilized to distinguish above salivary basaloid diseases from AdCC.Results: Histologically, the striking diagnostic features of cBCA, iBCA, SB, and IDH are composed of basaloid tumor cells, well-defined encapsulation, or lack of destructive invasion. Immunohistochemically, Myb immune-labeling could effectively make a distinction among cBCA, iBCA, SB, and IDH from AdCC, except in SB. cBCA and iBCA typically expressed β-catenin in the nuclei of tumor cells. There was no statistical significance in the ki67 index between SB and AdCC, but their indices were significantly higher than those of iBCA and IDH (p < 0.05, p < 0.05, respectively). P63 and calponin immune-expression were observed in the basaloid or myoepithelial cells. CD117 were observed positively in cBCA, iBCA, SB, and AdCC, except in IDH. SOX10 were observed positively in all cases. No cases had fusion of MYB and NFIB detectable by FISH, except in AdCC.Conclusion: Considering their sensitivity and specificity, FISH-Myb and an immunohistochemical panel of MYB/β-catenin/ki67 would be an optimal choice for the differential diagnosis of these basaloid lesions.Clinical relevance: Some salivary basaloid tumor or tumor-like lesions have overlapping features with AdCC. Through this present research, we suggested that the panel IHC of MYB, βcatenin, and ki67 combined with FISH-Myb should be an optimal choice for differential diagnosis among those lesions.https://www.frontiersin.org/article/10.3389/fonc.2020.00870/fullMYBimmunohistochemistryfluorescence in situ hybridizationsalivary basaloid lesionsadenoid cystic carcinoma |
| spellingShingle | Binbin Li Binbin Li Binbin Li Weiping Jie Weiping Jie Weiping Jie Huiying He Myb Immunohistochemical Staining and Fluorescence in situ Hybridization in Salivary Rare Basaloid Lesions Frontiers in Oncology MYB immunohistochemistry fluorescence in situ hybridization salivary basaloid lesions adenoid cystic carcinoma |
| title | Myb Immunohistochemical Staining and Fluorescence in situ Hybridization in Salivary Rare Basaloid Lesions |
| title_full | Myb Immunohistochemical Staining and Fluorescence in situ Hybridization in Salivary Rare Basaloid Lesions |
| title_fullStr | Myb Immunohistochemical Staining and Fluorescence in situ Hybridization in Salivary Rare Basaloid Lesions |
| title_full_unstemmed | Myb Immunohistochemical Staining and Fluorescence in situ Hybridization in Salivary Rare Basaloid Lesions |
| title_short | Myb Immunohistochemical Staining and Fluorescence in situ Hybridization in Salivary Rare Basaloid Lesions |
| title_sort | myb immunohistochemical staining and fluorescence in situ hybridization in salivary rare basaloid lesions |
| topic | MYB immunohistochemistry fluorescence in situ hybridization salivary basaloid lesions adenoid cystic carcinoma |
| url | https://www.frontiersin.org/article/10.3389/fonc.2020.00870/full |
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