The Interaction of NO and H<sub>2</sub>S in Boar Spermatozoa under Oxidative Stress
Various recent studies dedicated to the role of nitric oxide (NO) and hydrogen sulfide (H<sub>2</sub>S) in somatic cells provide evidence for an interaction of the two gasotransmitters. In the case of male gametes, only the action of a single donor of each gasotransmitter has been invest...
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MDPI AG
2022-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/5/602 |
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| author | Martin Kadlec Eliana Pintus José Luis Ros-Santaella |
| author_facet | Martin Kadlec Eliana Pintus José Luis Ros-Santaella |
| author_sort | Martin Kadlec |
| collection | DOAJ |
| description | Various recent studies dedicated to the role of nitric oxide (NO) and hydrogen sulfide (H<sub>2</sub>S) in somatic cells provide evidence for an interaction of the two gasotransmitters. In the case of male gametes, only the action of a single donor of each gasotransmitter has been investigated up until today. It has been demonstrated that, at low concentrations, both gasotransmitters alone exert a positive effect on sperm quality parameters. Moreover, the activity of gaseous cellular messengers may be affected by the presence of oxidative stress, an underlying condition of several male reproductive disorders. In this study, we explored the effect of the combination of two donors SNP and NaHS (NO and H<sub>2</sub>S donors, respectively) on boar spermatozoa under oxidative stress. We applied NaHS, SNP, and their combination (DD) at 100 nM concentration in boar spermatozoa samples treated with Fe<sup>2+</sup>/ascorbate system. After 90 min of incubation at 38 °C, we have observed that progressive motility (PMot) and plasma membrane integrity (PMI) were improved (<i>p</i> < 0.05) in DD treatment compared to the Ctr sample under oxidative stress (CtrOX). Moreover, the PMot of DD treatment was higher (<i>p</i> < 0.05) than that of NaHS. Similar to NaHS, SNP treatment did not overcome the PMot and PMI of CtrOX. In conclusion, for the first time, we provide evidence that the combination of SNP and NaHS surmounts the effect of single-donor application in terms of PMot and PMI in porcine spermatozoa under oxidative stress. |
| first_indexed | 2024-03-09T20:49:29Z |
| format | Article |
| id | doaj.art-4f246da436b94f5da08e69529b231005 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T20:49:29Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-4f246da436b94f5da08e69529b2310052023-11-23T22:37:04ZengMDPI AGAnimals2076-26152022-02-0112560210.3390/ani12050602The Interaction of NO and H<sub>2</sub>S in Boar Spermatozoa under Oxidative StressMartin Kadlec0Eliana Pintus1José Luis Ros-Santaella2Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech RepublicDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech RepublicDepartment of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech RepublicVarious recent studies dedicated to the role of nitric oxide (NO) and hydrogen sulfide (H<sub>2</sub>S) in somatic cells provide evidence for an interaction of the two gasotransmitters. In the case of male gametes, only the action of a single donor of each gasotransmitter has been investigated up until today. It has been demonstrated that, at low concentrations, both gasotransmitters alone exert a positive effect on sperm quality parameters. Moreover, the activity of gaseous cellular messengers may be affected by the presence of oxidative stress, an underlying condition of several male reproductive disorders. In this study, we explored the effect of the combination of two donors SNP and NaHS (NO and H<sub>2</sub>S donors, respectively) on boar spermatozoa under oxidative stress. We applied NaHS, SNP, and their combination (DD) at 100 nM concentration in boar spermatozoa samples treated with Fe<sup>2+</sup>/ascorbate system. After 90 min of incubation at 38 °C, we have observed that progressive motility (PMot) and plasma membrane integrity (PMI) were improved (<i>p</i> < 0.05) in DD treatment compared to the Ctr sample under oxidative stress (CtrOX). Moreover, the PMot of DD treatment was higher (<i>p</i> < 0.05) than that of NaHS. Similar to NaHS, SNP treatment did not overcome the PMot and PMI of CtrOX. In conclusion, for the first time, we provide evidence that the combination of SNP and NaHS surmounts the effect of single-donor application in terms of PMot and PMI in porcine spermatozoa under oxidative stress.https://www.mdpi.com/2076-2615/12/5/602gasotransmitter interactionhydrogen sulfidenitric oxideoxidative stressboar spermatozoa |
| spellingShingle | Martin Kadlec Eliana Pintus José Luis Ros-Santaella The Interaction of NO and H<sub>2</sub>S in Boar Spermatozoa under Oxidative Stress Animals gasotransmitter interaction hydrogen sulfide nitric oxide oxidative stress boar spermatozoa |
| title | The Interaction of NO and H<sub>2</sub>S in Boar Spermatozoa under Oxidative Stress |
| title_full | The Interaction of NO and H<sub>2</sub>S in Boar Spermatozoa under Oxidative Stress |
| title_fullStr | The Interaction of NO and H<sub>2</sub>S in Boar Spermatozoa under Oxidative Stress |
| title_full_unstemmed | The Interaction of NO and H<sub>2</sub>S in Boar Spermatozoa under Oxidative Stress |
| title_short | The Interaction of NO and H<sub>2</sub>S in Boar Spermatozoa under Oxidative Stress |
| title_sort | interaction of no and h sub 2 sub s in boar spermatozoa under oxidative stress |
| topic | gasotransmitter interaction hydrogen sulfide nitric oxide oxidative stress boar spermatozoa |
| url | https://www.mdpi.com/2076-2615/12/5/602 |
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