Patterned progression of gut microbiota associated with necrotizing enterocolitis and late onset sepsis in preterm infants: a prospective study in a Chinese neonatal intensive care unit
Necrotizing enterocolitis (NEC) and late-onset sepsis (LOS) are two common premature birth complications with high morbidity and mortality. Recent studies in Europe and America have linked gut microbiota dysbiosis to their etiology. However, similar studies in Asian populations remain scant. In this...
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PeerJ Inc.
2019-07-01
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| author | Jiayi Liu Yuqing Li Yi Feng Liya Pan Zhoulonglong Xie Zhilong Yan Li Zhang Mingkun Li Jianhua Zhao Jianhua Sun Li Hong |
| author_facet | Jiayi Liu Yuqing Li Yi Feng Liya Pan Zhoulonglong Xie Zhilong Yan Li Zhang Mingkun Li Jianhua Zhao Jianhua Sun Li Hong |
| author_sort | Jiayi Liu |
| collection | DOAJ |
| description | Necrotizing enterocolitis (NEC) and late-onset sepsis (LOS) are two common premature birth complications with high morbidity and mortality. Recent studies in Europe and America have linked gut microbiota dysbiosis to their etiology. However, similar studies in Asian populations remain scant. In this pilot study, we profiled gut microbiota of 24 Chinese preterm infants from birth till death or discharge from NICU. Four of them developed NEC and three developed LOS. Unexpectedly, we detected highly-diversified microbiota with similar compositions in all patients shortly after birth. However, as patients aged, the microbial diversities in case groups differed significantly from that of the control group. These differences emerged after the third day of life and persisted throughout the course of both NEC and LOS. Using a Zero-Inflated Beta Regression Model with Random Effects (ZIBR), we detected higher Bacillus (p = 0.032) and Solibacillus (p = 0.047) before the onset of NEC and LOS. During NEC progression, Enterococcus, Streptococcus and Peptoclostridium were the dominant genera while during LOS progression; Klebsiella was the only dominant genus that was also detected by the diagnostic hemoculture. These results warrant further studies to identify causative microbial patterns and underlying mechanisms. |
| first_indexed | 2024-03-09T07:52:32Z |
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| id | doaj.art-4f25badf4f274925a8b1aef0eed9fc4d |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T07:52:32Z |
| publishDate | 2019-07-01 |
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| spelling | doaj.art-4f25badf4f274925a8b1aef0eed9fc4d2023-12-03T01:26:17ZengPeerJ Inc.PeerJ2167-83592019-07-017e731010.7717/peerj.7310Patterned progression of gut microbiota associated with necrotizing enterocolitis and late onset sepsis in preterm infants: a prospective study in a Chinese neonatal intensive care unitJiayi Liu0Yuqing Li1Yi Feng2Liya Pan3Zhoulonglong Xie4Zhilong Yan5Li Zhang6Mingkun Li7Jianhua Zhao8Jianhua Sun9Li Hong10Department of Clinical Nutrition, Shanghai Children’s Medical Center, School of Medicine Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Nutrition, Shanghai Children’s Medical Center, School of Medicine Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Nutrition, Shanghai Children’s Medical Center, School of Medicine Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Nutrition, Shanghai Children’s Medical Center, School of Medicine Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Surgery, Shanghai Children’s Medical Center, School of Medicine Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Surgery, Shanghai Children’s Medical Center, School of Medicine Shanghai Jiao Tong University, Shanghai, ChinaKey Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Genomic and Precision Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, ChinaShanghai Majorbio Bio-pharm Technology Co., Ltd, Shanghai, ChinaDepartment of Neonatology, Shanghai Children’s Medical Center, School of Medicine Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Clinical Nutrition, Shanghai Children’s Medical Center, School of Medicine Shanghai Jiao Tong University, Shanghai, ChinaNecrotizing enterocolitis (NEC) and late-onset sepsis (LOS) are two common premature birth complications with high morbidity and mortality. Recent studies in Europe and America have linked gut microbiota dysbiosis to their etiology. However, similar studies in Asian populations remain scant. In this pilot study, we profiled gut microbiota of 24 Chinese preterm infants from birth till death or discharge from NICU. Four of them developed NEC and three developed LOS. Unexpectedly, we detected highly-diversified microbiota with similar compositions in all patients shortly after birth. However, as patients aged, the microbial diversities in case groups differed significantly from that of the control group. These differences emerged after the third day of life and persisted throughout the course of both NEC and LOS. Using a Zero-Inflated Beta Regression Model with Random Effects (ZIBR), we detected higher Bacillus (p = 0.032) and Solibacillus (p = 0.047) before the onset of NEC and LOS. During NEC progression, Enterococcus, Streptococcus and Peptoclostridium were the dominant genera while during LOS progression; Klebsiella was the only dominant genus that was also detected by the diagnostic hemoculture. These results warrant further studies to identify causative microbial patterns and underlying mechanisms.https://peerj.com/articles/7310.pdfGut MicrobiotaPreterm InfantNecrotizing EnterocolitisNeonatal Late Onset SepsisHigh Throughput DNA Sequencing |
| spellingShingle | Jiayi Liu Yuqing Li Yi Feng Liya Pan Zhoulonglong Xie Zhilong Yan Li Zhang Mingkun Li Jianhua Zhao Jianhua Sun Li Hong Patterned progression of gut microbiota associated with necrotizing enterocolitis and late onset sepsis in preterm infants: a prospective study in a Chinese neonatal intensive care unit PeerJ Gut Microbiota Preterm Infant Necrotizing Enterocolitis Neonatal Late Onset Sepsis High Throughput DNA Sequencing |
| title | Patterned progression of gut microbiota associated with necrotizing enterocolitis and late onset sepsis in preterm infants: a prospective study in a Chinese neonatal intensive care unit |
| title_full | Patterned progression of gut microbiota associated with necrotizing enterocolitis and late onset sepsis in preterm infants: a prospective study in a Chinese neonatal intensive care unit |
| title_fullStr | Patterned progression of gut microbiota associated with necrotizing enterocolitis and late onset sepsis in preterm infants: a prospective study in a Chinese neonatal intensive care unit |
| title_full_unstemmed | Patterned progression of gut microbiota associated with necrotizing enterocolitis and late onset sepsis in preterm infants: a prospective study in a Chinese neonatal intensive care unit |
| title_short | Patterned progression of gut microbiota associated with necrotizing enterocolitis and late onset sepsis in preterm infants: a prospective study in a Chinese neonatal intensive care unit |
| title_sort | patterned progression of gut microbiota associated with necrotizing enterocolitis and late onset sepsis in preterm infants a prospective study in a chinese neonatal intensive care unit |
| topic | Gut Microbiota Preterm Infant Necrotizing Enterocolitis Neonatal Late Onset Sepsis High Throughput DNA Sequencing |
| url | https://peerj.com/articles/7310.pdf |
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