Unraveling the antimicrobial potential of Lactiplantibacillus plantarum strains TE0907 and TE1809 sourced from Bufo gargarizans: advancing the frontier of probiotic-based therapeutics
IntroductionIn an era increasingly defined by the challenge of antibiotic resistance, this study offers groundbreaking insights into the antibacterial properties of two distinct Lactiplantibacillus plantarum strains, TE0907 and TE1809, hailing from the unique ecosystem of Bufo gargarizans. It unique...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1347830/full |
| _version_ | 1797311644044361728 |
|---|---|
| author | Feiyun Huang Yanni Zhao Yusen Hou Yu Yang Bisong Yue Xiuyue Zhang |
| author_facet | Feiyun Huang Yanni Zhao Yusen Hou Yu Yang Bisong Yue Xiuyue Zhang |
| author_sort | Feiyun Huang |
| collection | DOAJ |
| description | IntroductionIn an era increasingly defined by the challenge of antibiotic resistance, this study offers groundbreaking insights into the antibacterial properties of two distinct Lactiplantibacillus plantarum strains, TE0907 and TE1809, hailing from the unique ecosystem of Bufo gargarizans. It uniquely focuses on elucidating the intricate components and mechanisms that empower these strains with their notable antibacterial capabilities.MethodsThe research employs a multi-omics approach, including agar diffusion tests to assess antibacterial efficacy and adhesion assays with HT-29 cells to understand the preliminary mechanisms. Additionally, gas chromatography-mass spectrometry (GC-MS) is employed to analyze the production of organic acids, notably acetic acid, and whole-genome sequencing is utilized to identify genes linked to the biosynthesis of antibiotics and bacteriocin-coding domains.ResultsThe comparative analysis highlighted the exceptional antibacterial efficacy of strains TE0907 and TE1809, with mean inhibitory zones measured at 14.97 and 15.98 mm, respectively. A pivotal discovery was the significant synthesis of acetic acid in both strains, demonstrated by a robust correlation coefficient (cor ≥ 0.943), linking its abundance to their antimicrobial efficiency. Genomic exploration uncovered a diverse range of elements involved in the biosynthesis of antibiotics similar to tetracycline and vancomycin and potential regions encoding bacteriocins, including Enterolysin and Plantaricin.ConclusionThis research illuminates the remarkable antibacterial efficacy and mechanisms intrinsic to L. plantarum strains TE0907 and TE1809, sourced from B. gargarizans. The findings underscore the strains' extensive biochemical and enzymatic armamentarium, offering valuable insights into their role in antagonizing enteric pathogens. These results lay down a comprehensive analytical foundation for the potential clinical deployment of these strains in safeguarding animal gut health, thereby enriching our understanding of the role of probiotic bacteria in the realm of antimicrobial interventions. |
| first_indexed | 2024-03-08T02:02:38Z |
| format | Article |
| id | doaj.art-4f264a8d94264bb8828605d6ddf31e1d |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-08T02:02:38Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4f264a8d94264bb8828605d6ddf31e1d2024-02-14T04:48:35ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-02-011510.3389/fmicb.2024.13478301347830Unraveling the antimicrobial potential of Lactiplantibacillus plantarum strains TE0907 and TE1809 sourced from Bufo gargarizans: advancing the frontier of probiotic-based therapeuticsFeiyun Huang0Yanni Zhao1Yusen Hou2Yu Yang3Bisong Yue4Xiuyue Zhang5Key Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, ChinaKey Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, ChinaKey Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, ChinaKey Laboratory of Bio-Resources and Eco-Environment (Ministry of Education), College of Life Sciences, Sichuan University, Chengdu, ChinaSichuan Key Laboratory of Conservation Biology on Endangered Wildlife, College of Life Sciences, Sichuan University, Chengdu, ChinaIntroductionIn an era increasingly defined by the challenge of antibiotic resistance, this study offers groundbreaking insights into the antibacterial properties of two distinct Lactiplantibacillus plantarum strains, TE0907 and TE1809, hailing from the unique ecosystem of Bufo gargarizans. It uniquely focuses on elucidating the intricate components and mechanisms that empower these strains with their notable antibacterial capabilities.MethodsThe research employs a multi-omics approach, including agar diffusion tests to assess antibacterial efficacy and adhesion assays with HT-29 cells to understand the preliminary mechanisms. Additionally, gas chromatography-mass spectrometry (GC-MS) is employed to analyze the production of organic acids, notably acetic acid, and whole-genome sequencing is utilized to identify genes linked to the biosynthesis of antibiotics and bacteriocin-coding domains.ResultsThe comparative analysis highlighted the exceptional antibacterial efficacy of strains TE0907 and TE1809, with mean inhibitory zones measured at 14.97 and 15.98 mm, respectively. A pivotal discovery was the significant synthesis of acetic acid in both strains, demonstrated by a robust correlation coefficient (cor ≥ 0.943), linking its abundance to their antimicrobial efficiency. Genomic exploration uncovered a diverse range of elements involved in the biosynthesis of antibiotics similar to tetracycline and vancomycin and potential regions encoding bacteriocins, including Enterolysin and Plantaricin.ConclusionThis research illuminates the remarkable antibacterial efficacy and mechanisms intrinsic to L. plantarum strains TE0907 and TE1809, sourced from B. gargarizans. The findings underscore the strains' extensive biochemical and enzymatic armamentarium, offering valuable insights into their role in antagonizing enteric pathogens. These results lay down a comprehensive analytical foundation for the potential clinical deployment of these strains in safeguarding animal gut health, thereby enriching our understanding of the role of probiotic bacteria in the realm of antimicrobial interventions.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1347830/fullBufo gargarizansLactiplantibacillus plantarumSCFAsbacteriocinGC-MSwhole-genome sequencing |
| spellingShingle | Feiyun Huang Yanni Zhao Yusen Hou Yu Yang Bisong Yue Xiuyue Zhang Unraveling the antimicrobial potential of Lactiplantibacillus plantarum strains TE0907 and TE1809 sourced from Bufo gargarizans: advancing the frontier of probiotic-based therapeutics Frontiers in Microbiology Bufo gargarizans Lactiplantibacillus plantarum SCFAs bacteriocin GC-MS whole-genome sequencing |
| title | Unraveling the antimicrobial potential of Lactiplantibacillus plantarum strains TE0907 and TE1809 sourced from Bufo gargarizans: advancing the frontier of probiotic-based therapeutics |
| title_full | Unraveling the antimicrobial potential of Lactiplantibacillus plantarum strains TE0907 and TE1809 sourced from Bufo gargarizans: advancing the frontier of probiotic-based therapeutics |
| title_fullStr | Unraveling the antimicrobial potential of Lactiplantibacillus plantarum strains TE0907 and TE1809 sourced from Bufo gargarizans: advancing the frontier of probiotic-based therapeutics |
| title_full_unstemmed | Unraveling the antimicrobial potential of Lactiplantibacillus plantarum strains TE0907 and TE1809 sourced from Bufo gargarizans: advancing the frontier of probiotic-based therapeutics |
| title_short | Unraveling the antimicrobial potential of Lactiplantibacillus plantarum strains TE0907 and TE1809 sourced from Bufo gargarizans: advancing the frontier of probiotic-based therapeutics |
| title_sort | unraveling the antimicrobial potential of lactiplantibacillus plantarum strains te0907 and te1809 sourced from bufo gargarizans advancing the frontier of probiotic based therapeutics |
| topic | Bufo gargarizans Lactiplantibacillus plantarum SCFAs bacteriocin GC-MS whole-genome sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1347830/full |
| work_keys_str_mv | AT feiyunhuang unravelingtheantimicrobialpotentialoflactiplantibacillusplantarumstrainste0907andte1809sourcedfrombufogargarizansadvancingthefrontierofprobioticbasedtherapeutics AT yannizhao unravelingtheantimicrobialpotentialoflactiplantibacillusplantarumstrainste0907andte1809sourcedfrombufogargarizansadvancingthefrontierofprobioticbasedtherapeutics AT yusenhou unravelingtheantimicrobialpotentialoflactiplantibacillusplantarumstrainste0907andte1809sourcedfrombufogargarizansadvancingthefrontierofprobioticbasedtherapeutics AT yuyang unravelingtheantimicrobialpotentialoflactiplantibacillusplantarumstrainste0907andte1809sourcedfrombufogargarizansadvancingthefrontierofprobioticbasedtherapeutics AT bisongyue unravelingtheantimicrobialpotentialoflactiplantibacillusplantarumstrainste0907andte1809sourcedfrombufogargarizansadvancingthefrontierofprobioticbasedtherapeutics AT xiuyuezhang unravelingtheantimicrobialpotentialoflactiplantibacillusplantarumstrainste0907andte1809sourcedfrombufogargarizansadvancingthefrontierofprobioticbasedtherapeutics |