Comparative Metabolomic Analysis of Four Fabaceae and Relationship to In Vitro Nematicidal Activity against <i>Xiphinema index</i>
The grapevine fanleaf virus (GFLV), responsible for fanleaf degeneration, is spread in vineyards by the soil nematode <i>Xiphinema index</i>. Nematicide molecules were used to limit the spread of the disease until they were banned due to negative environmental impacts. Therefore, there i...
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MDPI AG
2022-05-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/10/3052 |
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| author | Lise Negrel Raymonde Baltenweck Gerard Demangeat Françoise Le Bohec-Dorner Camille Rustenholz Amandine Velt Claude Gertz Eva Bieler Markus Dürrenberger Pascale Gombault Philippe Hugueney Olivier Lemaire |
| author_facet | Lise Negrel Raymonde Baltenweck Gerard Demangeat Françoise Le Bohec-Dorner Camille Rustenholz Amandine Velt Claude Gertz Eva Bieler Markus Dürrenberger Pascale Gombault Philippe Hugueney Olivier Lemaire |
| author_sort | Lise Negrel |
| collection | DOAJ |
| description | The grapevine fanleaf virus (GFLV), responsible for fanleaf degeneration, is spread in vineyards by the soil nematode <i>Xiphinema index</i>. Nematicide molecules were used to limit the spread of the disease until they were banned due to negative environmental impacts. Therefore, there is a growing interest in alternative methods, including plant-derived products with antagonistic effects to <i>X. index</i>. In this work, we evaluated the nematicidal potential of the aerial parts and roots of four <i>Fabaceae</i>: sainfoin (<i>Onobrychis viciifolia)</i>, birdsfoot trefoil (<i>Lotus corniculatus)</i>, sweet clover (<i>Melilotus albus)</i>, and red clover (<i>Trifolium pratense)</i>, as well as that of sainfoin-based commercial pellets. For all tested plants, either aerial or root parts, or both of them, exhibited a nematicidal effect on <i>X. index</i> in vitro, pellets being as effective as freshly harvested plants. Comparative metabolomic analyses did not reveal molecules or molecule families specifically associated with antagonistic properties toward <i>X. index</i>, suggesting that the nematicidal effect is the result of a combination of different molecules rather than associated with a single compound. Finally, scanning electron microscope observations did not reveal the visible impact of <i>O. viciifolia</i> extract on <i>X. index</i> cuticle, suggesting that alteration of the cuticle may not be the primary cause of their nematicidal effect. |
| first_indexed | 2024-03-10T03:20:59Z |
| format | Article |
| id | doaj.art-4f26803661dc4323af9f6855286965e0 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T03:20:59Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4f26803661dc4323af9f6855286965e02023-11-23T12:20:17ZengMDPI AGMolecules1420-30492022-05-012710305210.3390/molecules27103052Comparative Metabolomic Analysis of Four Fabaceae and Relationship to In Vitro Nematicidal Activity against <i>Xiphinema index</i>Lise Negrel0Raymonde Baltenweck1Gerard Demangeat2Françoise Le Bohec-Dorner3Camille Rustenholz4Amandine Velt5Claude Gertz6Eva Bieler7Markus Dürrenberger8Pascale Gombault9Philippe Hugueney10Olivier Lemaire11Multifolia, 10408 Viâpres-le-Petit, FranceSanté de la Vigne et Qualité du Vin, INRAE, Université de Strasbourg, 68000 Colmar, FranceSanté de la Vigne et Qualité du Vin, INRAE, Université de Strasbourg, 68000 Colmar, FranceSanté de la Vigne et Qualité du Vin, INRAE, Université de Strasbourg, 68000 Colmar, FranceSanté de la Vigne et Qualité du Vin, INRAE, Université de Strasbourg, 68000 Colmar, FranceSanté de la Vigne et Qualité du Vin, INRAE, Université de Strasbourg, 68000 Colmar, FranceSanté de la Vigne et Qualité du Vin, INRAE, Université de Strasbourg, 68000 Colmar, FranceNano Imaging Laboratory, Swiss Nanoscience Institute, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, SwitzerlandNano Imaging Laboratory, Swiss Nanoscience Institute, University of Basel, Klingelbergstrasse 50/70, CH-4056 Basel, SwitzerlandMultifolia, 10408 Viâpres-le-Petit, FranceSanté de la Vigne et Qualité du Vin, INRAE, Université de Strasbourg, 68000 Colmar, FranceSanté de la Vigne et Qualité du Vin, INRAE, Université de Strasbourg, 68000 Colmar, FranceThe grapevine fanleaf virus (GFLV), responsible for fanleaf degeneration, is spread in vineyards by the soil nematode <i>Xiphinema index</i>. Nematicide molecules were used to limit the spread of the disease until they were banned due to negative environmental impacts. Therefore, there is a growing interest in alternative methods, including plant-derived products with antagonistic effects to <i>X. index</i>. In this work, we evaluated the nematicidal potential of the aerial parts and roots of four <i>Fabaceae</i>: sainfoin (<i>Onobrychis viciifolia)</i>, birdsfoot trefoil (<i>Lotus corniculatus)</i>, sweet clover (<i>Melilotus albus)</i>, and red clover (<i>Trifolium pratense)</i>, as well as that of sainfoin-based commercial pellets. For all tested plants, either aerial or root parts, or both of them, exhibited a nematicidal effect on <i>X. index</i> in vitro, pellets being as effective as freshly harvested plants. Comparative metabolomic analyses did not reveal molecules or molecule families specifically associated with antagonistic properties toward <i>X. index</i>, suggesting that the nematicidal effect is the result of a combination of different molecules rather than associated with a single compound. Finally, scanning electron microscope observations did not reveal the visible impact of <i>O. viciifolia</i> extract on <i>X. index</i> cuticle, suggesting that alteration of the cuticle may not be the primary cause of their nematicidal effect.https://www.mdpi.com/1420-3049/27/10/3052<i>Fabaceae</i><i>Onobrychis</i><i>Lotus</i><i>Melilotus</i><i>Trifolium</i><i>Xiphinema index</i> |
| spellingShingle | Lise Negrel Raymonde Baltenweck Gerard Demangeat Françoise Le Bohec-Dorner Camille Rustenholz Amandine Velt Claude Gertz Eva Bieler Markus Dürrenberger Pascale Gombault Philippe Hugueney Olivier Lemaire Comparative Metabolomic Analysis of Four Fabaceae and Relationship to In Vitro Nematicidal Activity against <i>Xiphinema index</i> Molecules <i>Fabaceae</i> <i>Onobrychis</i> <i>Lotus</i> <i>Melilotus</i> <i>Trifolium</i> <i>Xiphinema index</i> |
| title | Comparative Metabolomic Analysis of Four Fabaceae and Relationship to In Vitro Nematicidal Activity against <i>Xiphinema index</i> |
| title_full | Comparative Metabolomic Analysis of Four Fabaceae and Relationship to In Vitro Nematicidal Activity against <i>Xiphinema index</i> |
| title_fullStr | Comparative Metabolomic Analysis of Four Fabaceae and Relationship to In Vitro Nematicidal Activity against <i>Xiphinema index</i> |
| title_full_unstemmed | Comparative Metabolomic Analysis of Four Fabaceae and Relationship to In Vitro Nematicidal Activity against <i>Xiphinema index</i> |
| title_short | Comparative Metabolomic Analysis of Four Fabaceae and Relationship to In Vitro Nematicidal Activity against <i>Xiphinema index</i> |
| title_sort | comparative metabolomic analysis of four fabaceae and relationship to in vitro nematicidal activity against i xiphinema index i |
| topic | <i>Fabaceae</i> <i>Onobrychis</i> <i>Lotus</i> <i>Melilotus</i> <i>Trifolium</i> <i>Xiphinema index</i> |
| url | https://www.mdpi.com/1420-3049/27/10/3052 |
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