Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in south of Iran
In this study amylase producing actinomycetes were isolated from the sediments of mangrove forests in the south of Iran and the rate of amylase activity was measured. The samples of sediments were collected from one hundred different places in mangrove forests of the south of Iran. Collected samples...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2015-01-01
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| Series: | Egyptian Journal of Aquatic Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687428515000412 |
| _version_ | 1819055651951738880 |
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| author | Farshid Kafilzadeh Faranak Dehdari |
| author_facet | Farshid Kafilzadeh Faranak Dehdari |
| author_sort | Farshid Kafilzadeh |
| collection | DOAJ |
| description | In this study amylase producing actinomycetes were isolated from the sediments of mangrove forests in the south of Iran and the rate of amylase activity was measured. The samples of sediments were collected from one hundred different places in mangrove forests of the south of Iran. Collected samples were diluted then they were purified on the starch (casein agar) culture and Woodruff. After that they were examined in terms of amylase production on agar–starch culture. The activity of the produced amylase by the isolated aquatic actinomycetes was measured by dinitrosalicylic acid (DNS) method. The results showed that aquatic actinomycetes were isolated from 86 per 100 places in spring (86%) and from 61 per 100 places in summer (61%). The highest rates of producing enzyme were related to isolated samples in spring (62.97 U/ml). Biochemical and Bergey’s book tests showed that the most isolated aquatic actinomycetes belonged to Streptomyces genus. As regards this, it is economical and easy to isolate the aquatic actinomycetes which produce amylase that is used in different industries in Iran from the sediments of mangrove forests of the south of Iran. So the isolated strains in this study can be suitable candidates for amylase production after genetic manipulation. |
| first_indexed | 2024-12-21T13:10:55Z |
| format | Article |
| id | doaj.art-4f275785be3b47a1bff718d30520c97d |
| institution | Directory Open Access Journal |
| issn | 1687-4285 |
| language | English |
| last_indexed | 2024-12-21T13:10:55Z |
| publishDate | 2015-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Egyptian Journal of Aquatic Research |
| spelling | doaj.art-4f275785be3b47a1bff718d30520c97d2022-12-21T19:02:53ZengElsevierEgyptian Journal of Aquatic Research1687-42852015-01-0141219720110.1016/j.ejar.2015.04.003Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in south of IranFarshid KafilzadehFaranak DehdariIn this study amylase producing actinomycetes were isolated from the sediments of mangrove forests in the south of Iran and the rate of amylase activity was measured. The samples of sediments were collected from one hundred different places in mangrove forests of the south of Iran. Collected samples were diluted then they were purified on the starch (casein agar) culture and Woodruff. After that they were examined in terms of amylase production on agar–starch culture. The activity of the produced amylase by the isolated aquatic actinomycetes was measured by dinitrosalicylic acid (DNS) method. The results showed that aquatic actinomycetes were isolated from 86 per 100 places in spring (86%) and from 61 per 100 places in summer (61%). The highest rates of producing enzyme were related to isolated samples in spring (62.97 U/ml). Biochemical and Bergey’s book tests showed that the most isolated aquatic actinomycetes belonged to Streptomyces genus. As regards this, it is economical and easy to isolate the aquatic actinomycetes which produce amylase that is used in different industries in Iran from the sediments of mangrove forests of the south of Iran. So the isolated strains in this study can be suitable candidates for amylase production after genetic manipulation.http://www.sciencedirect.com/science/article/pii/S1687428515000412Mangrove forestsAmylaseDinitrosalicylic acid methodStreptomyces |
| spellingShingle | Farshid Kafilzadeh Faranak Dehdari Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in south of Iran Egyptian Journal of Aquatic Research Mangrove forests Amylase Dinitrosalicylic acid method Streptomyces |
| title | Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in south of Iran |
| title_full | Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in south of Iran |
| title_fullStr | Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in south of Iran |
| title_full_unstemmed | Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in south of Iran |
| title_short | Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in south of Iran |
| title_sort | amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in south of iran |
| topic | Mangrove forests Amylase Dinitrosalicylic acid method Streptomyces |
| url | http://www.sciencedirect.com/science/article/pii/S1687428515000412 |
| work_keys_str_mv | AT farshidkafilzadeh amylaseactivityofaquaticactinomycetesisolatedfromthesedimentsofmangroveforestsinsouthofiran AT faranakdehdari amylaseactivityofaquaticactinomycetesisolatedfromthesedimentsofmangroveforestsinsouthofiran |