Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice
Abstract INTRODUCTION: Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects. METHODS Food samples were experimentally contaminated with insects,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2019-12-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100605&tlng=en |
| _version_ | 1818534659862036480 |
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| author | Elaine Cristina de Mattos Maria Aparecida Moraes Marciano Vilma dos Santos Menezes Gaiotto Daros Cristiane Castro Faccini Angela Maria Lourenço Vera Lucia Pereira-Chioccola |
| author_facet | Elaine Cristina de Mattos Maria Aparecida Moraes Marciano Vilma dos Santos Menezes Gaiotto Daros Cristiane Castro Faccini Angela Maria Lourenço Vera Lucia Pereira-Chioccola |
| author_sort | Elaine Cristina de Mattos |
| collection | DOAJ |
| description | Abstract INTRODUCTION: Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects. METHODS Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR). RESULTS: Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food. CONCLUSIONS: This methodology could be utilized by food analysts to identify possible insect contamination in food samples. |
| first_indexed | 2024-12-11T18:14:27Z |
| format | Article |
| id | doaj.art-4f277c21d11c419a8cdf5e43ae2a47c2 |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-12-11T18:14:27Z |
| publishDate | 2019-12-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-4f277c21d11c419a8cdf5e43ae2a47c22022-12-22T00:55:28ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492019-12-015310.1590/0037-8682-0119-2019Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juiceElaine Cristina de Mattoshttps://orcid.org/0000-0002-1052-8883Maria Aparecida Moraes MarcianoVilma dos Santos Menezes Gaiotto DarosCristiane Castro FacciniAngela Maria LourençoVera Lucia Pereira-ChioccolaAbstract INTRODUCTION: Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects. METHODS Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR). RESULTS: Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food. CONCLUSIONS: This methodology could be utilized by food analysts to identify possible insect contamination in food samples.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100605&tlng=enColeopteraEuterpeTriatominaePolymerase Chain ReactionFoodFoodborne Diseases |
| spellingShingle | Elaine Cristina de Mattos Maria Aparecida Moraes Marciano Vilma dos Santos Menezes Gaiotto Daros Cristiane Castro Faccini Angela Maria Lourenço Vera Lucia Pereira-Chioccola Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice Revista da Sociedade Brasileira de Medicina Tropical Coleoptera Euterpe Triatominae Polymerase Chain Reaction Food Foodborne Diseases |
| title | Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice |
| title_full | Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice |
| title_fullStr | Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice |
| title_full_unstemmed | Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice |
| title_short | Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice |
| title_sort | fragment detection of coleopteran and triatomine insects in experimentally contaminated acai pulp and sugarcane juice |
| topic | Coleoptera Euterpe Triatominae Polymerase Chain Reaction Food Foodborne Diseases |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822020000100605&tlng=en |
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