Light response of Vibrio parahaemolyticus
Light is one of the most critical stimuli in the majority of living organisms. In the last two decades, blue light (BL) has become a major subject of attention because of developments in light-emitting diodes (LED). The effects of BL on eukaryotic organisms and phototrophic prokaryotes have been wel...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Marine Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2022.1037594/full |
| _version_ | 1828134750623105024 |
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| author | Yunato Kuroyanagi Jiro Tsuchiya Chunqi Jiang Sayaka Mino Hisae Kasai Daisuke Motooka Tetsuya Iida Tetsuya Iida Tetsuya Iida Masataka Satomi Tomoo Sawabe |
| author_facet | Yunato Kuroyanagi Jiro Tsuchiya Chunqi Jiang Sayaka Mino Hisae Kasai Daisuke Motooka Tetsuya Iida Tetsuya Iida Tetsuya Iida Masataka Satomi Tomoo Sawabe |
| author_sort | Yunato Kuroyanagi |
| collection | DOAJ |
| description | Light is one of the most critical stimuli in the majority of living organisms. In the last two decades, blue light (BL) has become a major subject of attention because of developments in light-emitting diodes (LED). The effects of BL on eukaryotic organisms and phototrophic prokaryotes have been well studied, but the knowledge of its effects on non-phototrophic prokaryotes remains unclear. Since BL can penetrate seawater, it is expected that most prokaryotes living in the ocean possess molecular mechanisms which protect against BL. The aim of this study is to assess the molecular mechanisms of Vibrio parahaemolyticus cells against BL as a marine bacterial model compared to other wavelength light exposures. Physiological and transcriptomic analyses of BL-exposed cells compared to other light treated cells revealed the highest ROS fold change, the highest number of differentially expressed genes (DEGs), and up-regulation in the gene responsible to not only compatible solute such as glycine betaine and ectoine but also iron-sulfur biosynthesis related to ROS formation. Furthermore, red light (RL) up-regulated the expression of cryptochrome DASH, a protein known to be excited by BL, and orange light (OL) decreased the expression of thermostable direct hemolysin (TDH), suggesting that OL attenuates the virulence of V. parahaemolyticus. In addition, the expression of VtrA (V. parahaemolyticus type III secretion system 2 (T3SS2) regulator A) but not VtrB (V. parahaemolyticus T3SS2 regulator B) increased under both light treatments, indicating that light exposure is unlikely to be involved in T3SS2-mediated pathogenicity. These results expand our knowledge on unique light responses in non-phototrophic marine prokaryotes. |
| first_indexed | 2024-04-11T17:39:43Z |
| format | Article |
| id | doaj.art-4f2a2e285cf5466ebae29818a85caa17 |
| institution | Directory Open Access Journal |
| issn | 2296-7745 |
| language | English |
| last_indexed | 2024-04-11T17:39:43Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj.art-4f2a2e285cf5466ebae29818a85caa172022-12-22T04:11:31ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452022-11-01910.3389/fmars.2022.10375941037594Light response of Vibrio parahaemolyticusYunato Kuroyanagi0Jiro Tsuchiya1Chunqi Jiang2Sayaka Mino3Hisae Kasai4Daisuke Motooka5Tetsuya Iida6Tetsuya Iida7Tetsuya Iida8Masataka Satomi9Tomoo Sawabe10Laboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, JapanLaboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, JapanLaboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, JapanLaboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, JapanLaboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, JapanDepartment of Infection Metagenomics, Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Osaka, JapanDepartment of Infection Metagenomics, Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Osaka, JapanDepartment of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Osaka, JapanCenter for Infectious Disease Education and Research (CiDER), Osaka University, Osaka, JapanJapan Fisheries Research and Education Agency, Fisheries Technology Institute, Shizuoka, JapanLaboratory of Microbiology, Faculty of Fisheries Sciences, Hokkaido University, Hakodate, JapanLight is one of the most critical stimuli in the majority of living organisms. In the last two decades, blue light (BL) has become a major subject of attention because of developments in light-emitting diodes (LED). The effects of BL on eukaryotic organisms and phototrophic prokaryotes have been well studied, but the knowledge of its effects on non-phototrophic prokaryotes remains unclear. Since BL can penetrate seawater, it is expected that most prokaryotes living in the ocean possess molecular mechanisms which protect against BL. The aim of this study is to assess the molecular mechanisms of Vibrio parahaemolyticus cells against BL as a marine bacterial model compared to other wavelength light exposures. Physiological and transcriptomic analyses of BL-exposed cells compared to other light treated cells revealed the highest ROS fold change, the highest number of differentially expressed genes (DEGs), and up-regulation in the gene responsible to not only compatible solute such as glycine betaine and ectoine but also iron-sulfur biosynthesis related to ROS formation. Furthermore, red light (RL) up-regulated the expression of cryptochrome DASH, a protein known to be excited by BL, and orange light (OL) decreased the expression of thermostable direct hemolysin (TDH), suggesting that OL attenuates the virulence of V. parahaemolyticus. In addition, the expression of VtrA (V. parahaemolyticus type III secretion system 2 (T3SS2) regulator A) but not VtrB (V. parahaemolyticus T3SS2 regulator B) increased under both light treatments, indicating that light exposure is unlikely to be involved in T3SS2-mediated pathogenicity. These results expand our knowledge on unique light responses in non-phototrophic marine prokaryotes.https://www.frontiersin.org/articles/10.3389/fmars.2022.1037594/fullblue lightvibrio parahaemolyticusROSRNA-Seqlight response |
| spellingShingle | Yunato Kuroyanagi Jiro Tsuchiya Chunqi Jiang Sayaka Mino Hisae Kasai Daisuke Motooka Tetsuya Iida Tetsuya Iida Tetsuya Iida Masataka Satomi Tomoo Sawabe Light response of Vibrio parahaemolyticus Frontiers in Marine Science blue light vibrio parahaemolyticus ROS RNA-Seq light response |
| title | Light response of Vibrio parahaemolyticus |
| title_full | Light response of Vibrio parahaemolyticus |
| title_fullStr | Light response of Vibrio parahaemolyticus |
| title_full_unstemmed | Light response of Vibrio parahaemolyticus |
| title_short | Light response of Vibrio parahaemolyticus |
| title_sort | light response of vibrio parahaemolyticus |
| topic | blue light vibrio parahaemolyticus ROS RNA-Seq light response |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2022.1037594/full |
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