Joint Development Involves a Continuous Influx of Gdf5-Positive Cells
Synovial joints comprise several tissue types, including articular cartilage, the capsule, and ligaments. All of these compartments are commonly assumed to originate from an early set of Gdf5-expressing progenitors populating the interzone domain. Here, we provide evidence that joints develop throug...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-06-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124716306532 |
| _version_ | 1818265622130196480 |
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| author | Yulia Shwartz Sergey Viukov Sharon Krief Elazar Zelzer |
| author_facet | Yulia Shwartz Sergey Viukov Sharon Krief Elazar Zelzer |
| author_sort | Yulia Shwartz |
| collection | DOAJ |
| description | Synovial joints comprise several tissue types, including articular cartilage, the capsule, and ligaments. All of these compartments are commonly assumed to originate from an early set of Gdf5-expressing progenitors populating the interzone domain. Here, we provide evidence that joints develop through a continuous influx of cells into the interzone, where they contribute differentially to forming joint tissues. Using a knockin Gdf5-CreERT2 mouse, we show that early labeling of Gdf5-positive interzone cells failed to mark the entire organ. Conversely, multiple Cre activation steps indicated a contribution of these cells to various joint compartments later in development. Spatiotemporal differences between Gdf5 and tdTomato reporter expression support the notion of a continuous recruitment process. Finally, differential contribution of Gdf5-positive cells to various tissues suggests that the spatiotemporal dynamics of Gdf5 expression may instruct lineage divergence. This work supports the influx model of joint development, which may apply to other organogenic processes. |
| first_indexed | 2024-12-12T19:53:44Z |
| format | Article |
| id | doaj.art-4f2e37c9659c4009a0ec86eb1e036789 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-12T19:53:44Z |
| publishDate | 2016-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-4f2e37c9659c4009a0ec86eb1e0367892022-12-22T00:13:55ZengElsevierCell Reports2211-12472016-06-0115122577258710.1016/j.celrep.2016.05.055Joint Development Involves a Continuous Influx of Gdf5-Positive CellsYulia Shwartz0Sergey Viukov1Sharon Krief2Elazar Zelzer3Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, IsraelDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, IsraelDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, IsraelDepartment of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, IsraelSynovial joints comprise several tissue types, including articular cartilage, the capsule, and ligaments. All of these compartments are commonly assumed to originate from an early set of Gdf5-expressing progenitors populating the interzone domain. Here, we provide evidence that joints develop through a continuous influx of cells into the interzone, where they contribute differentially to forming joint tissues. Using a knockin Gdf5-CreERT2 mouse, we show that early labeling of Gdf5-positive interzone cells failed to mark the entire organ. Conversely, multiple Cre activation steps indicated a contribution of these cells to various joint compartments later in development. Spatiotemporal differences between Gdf5 and tdTomato reporter expression support the notion of a continuous recruitment process. Finally, differential contribution of Gdf5-positive cells to various tissues suggests that the spatiotemporal dynamics of Gdf5 expression may instruct lineage divergence. This work supports the influx model of joint development, which may apply to other organogenic processes.http://www.sciencedirect.com/science/article/pii/S2211124716306532Gdf5synovial jointskeletogenesisinterzoneprogenitor cellSox9lineage tracingmouse |
| spellingShingle | Yulia Shwartz Sergey Viukov Sharon Krief Elazar Zelzer Joint Development Involves a Continuous Influx of Gdf5-Positive Cells Cell Reports Gdf5 synovial joint skeletogenesis interzone progenitor cell Sox9 lineage tracing mouse |
| title | Joint Development Involves a Continuous Influx of Gdf5-Positive Cells |
| title_full | Joint Development Involves a Continuous Influx of Gdf5-Positive Cells |
| title_fullStr | Joint Development Involves a Continuous Influx of Gdf5-Positive Cells |
| title_full_unstemmed | Joint Development Involves a Continuous Influx of Gdf5-Positive Cells |
| title_short | Joint Development Involves a Continuous Influx of Gdf5-Positive Cells |
| title_sort | joint development involves a continuous influx of gdf5 positive cells |
| topic | Gdf5 synovial joint skeletogenesis interzone progenitor cell Sox9 lineage tracing mouse |
| url | http://www.sciencedirect.com/science/article/pii/S2211124716306532 |
| work_keys_str_mv | AT yuliashwartz jointdevelopmentinvolvesacontinuousinfluxofgdf5positivecells AT sergeyviukov jointdevelopmentinvolvesacontinuousinfluxofgdf5positivecells AT sharonkrief jointdevelopmentinvolvesacontinuousinfluxofgdf5positivecells AT elazarzelzer jointdevelopmentinvolvesacontinuousinfluxofgdf5positivecells |