A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture
A hypoxic (low oxygen level) microenvironment and nitric oxide paracrine signaling play important roles in the control of both biological and pathological cell responses. In this study, we present a microfluidic chip architecture for nitric oxide delivery under a hypoxic microenvironment in human em...
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| Format: | Article |
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MDPI AG
2020-10-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/11/979 |
| _version_ | 1827703113299001344 |
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| author | Samineh Barmaki Daniela Obermaier Esko Kankuri Jyrki Vuola Sami Franssila Ville Jokinen |
| author_facet | Samineh Barmaki Daniela Obermaier Esko Kankuri Jyrki Vuola Sami Franssila Ville Jokinen |
| author_sort | Samineh Barmaki |
| collection | DOAJ |
| description | A hypoxic (low oxygen level) microenvironment and nitric oxide paracrine signaling play important roles in the control of both biological and pathological cell responses. In this study, we present a microfluidic chip architecture for nitric oxide delivery under a hypoxic microenvironment in human embryonic kidney cells (HEK-293). The chip utilizes two separate, but interdigitated microfluidic channels. The hypoxic microenvironment was created by sodium sulfite as the oxygen scavenger in one of the channels. The nitric oxide microenvironment was created by sodium nitroprusside as the light-activated nitric oxide donor in the other channel. The solutions are separated from the cell culture by a 30 µm thick gas-permeable, but liquid-impermeable polydimethylsiloxane membrane. We show that the architecture is preliminarily feasible to define the gaseous microenvironment of a cell culture in the 100 µm and 1 mm length scales. |
| first_indexed | 2024-03-10T15:11:51Z |
| format | Article |
| id | doaj.art-4f2f0bf3a96a410ba3019a340d379e20 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T15:11:51Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-4f2f0bf3a96a410ba3019a340d379e202023-11-20T19:17:21ZengMDPI AGMicromachines2072-666X2020-10-01111197910.3390/mi11110979A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell CultureSamineh Barmaki0Daniela Obermaier1Esko Kankuri2Jyrki Vuola3Sami Franssila4Ville Jokinen5Department of Pharmacology, Faculty of Medicine, University of Helsinki, 00290 Helsinki, FinlandPreSens Precision Sensing GmbH, 93053 Regensburg, GermanyDepartment of Pharmacology, Faculty of Medicine, University of Helsinki, 00290 Helsinki, FinlandHelsinki Burn Centre, Jorvi Hospital, Helsinki University Hospital and University of Helsinki, 00260 Helsinki, FinlandDepartment of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, 00076 Espoo, FinlandDepartment of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, 00076 Espoo, FinlandA hypoxic (low oxygen level) microenvironment and nitric oxide paracrine signaling play important roles in the control of both biological and pathological cell responses. In this study, we present a microfluidic chip architecture for nitric oxide delivery under a hypoxic microenvironment in human embryonic kidney cells (HEK-293). The chip utilizes two separate, but interdigitated microfluidic channels. The hypoxic microenvironment was created by sodium sulfite as the oxygen scavenger in one of the channels. The nitric oxide microenvironment was created by sodium nitroprusside as the light-activated nitric oxide donor in the other channel. The solutions are separated from the cell culture by a 30 µm thick gas-permeable, but liquid-impermeable polydimethylsiloxane membrane. We show that the architecture is preliminarily feasible to define the gaseous microenvironment of a cell culture in the 100 µm and 1 mm length scales.https://www.mdpi.com/2072-666X/11/11/979hypoxianitric oxidemicroenvironmentcell culturemicrofluidic chipoxygen depletion |
| spellingShingle | Samineh Barmaki Daniela Obermaier Esko Kankuri Jyrki Vuola Sami Franssila Ville Jokinen A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture Micromachines hypoxia nitric oxide microenvironment cell culture microfluidic chip oxygen depletion |
| title | A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture |
| title_full | A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture |
| title_fullStr | A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture |
| title_full_unstemmed | A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture |
| title_short | A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture |
| title_sort | microfluidic chip architecture enabling a hypoxic microenvironment and nitric oxide delivery in cell culture |
| topic | hypoxia nitric oxide microenvironment cell culture microfluidic chip oxygen depletion |
| url | https://www.mdpi.com/2072-666X/11/11/979 |
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