Germacrone exerts anti-cancer effects on gastric cancer through induction of cell cycle arrest and promotion of apoptosis
Abstract Background Germacrone is one of the natural bioactive compounds found in Rhizoma curcuma essential oils. In this study, the potential anti-cancer effect of germacrone in gastric cancer cell line BGC823 was investigated. Methods The cell viability and proliferative activity were assessed, an...
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-019-2810-3 |
| _version_ | 1818456601491668992 |
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| author | Lei Wu Lifen Wang Xiangguo Tian Junyong Zhang Hua Feng |
| author_facet | Lei Wu Lifen Wang Xiangguo Tian Junyong Zhang Hua Feng |
| author_sort | Lei Wu |
| collection | DOAJ |
| description | Abstract Background Germacrone is one of the natural bioactive compounds found in Rhizoma curcuma essential oils. In this study, the potential anti-cancer effect of germacrone in gastric cancer cell line BGC823 was investigated. Methods The cell viability and proliferative activity were assessed, and cell cycle analysis was also performed. Hoechst 33258 and Annexin V/PI double staining was used for detection of cell apoptosis. Protein profiles of cell cycle-related and apoptosis-related proteins were assessed. Results MTT assay revealed that germacrone had marked cytotoxicity on BGC823 cells. Germacrone induced cell cycle arrest in the G2/M phase via remarkably decreased expression levels of cyclin B1, cdc 2 and cdc 25c. In addition, the treatment with germacrone induced caspase-3 activity and PARP cleavage. These findings demonstrated the effects of germacrone on inhibiting cell proliferation through induction of G2/M phase cell cycle arrest and promotion of cell apoptosis. It also indicated that germacrone functioned through modulations of cell cycle-associated protein expression and mitochondria-mediated apoptosis. Conclusion These findings will be valuable as the molecular basis for the germacrone-mediated anti-cancer effect against gastric cancer. |
| first_indexed | 2024-12-14T22:29:16Z |
| format | Article |
| id | doaj.art-4f2f955123204468ba97dfd97c1e9506 |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-12-14T22:29:16Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
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| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-4f2f955123204468ba97dfd97c1e95062022-12-21T22:45:16ZengBMCBMC Complementary Medicine and Therapies2662-76712020-01-012011910.1186/s12906-019-2810-3Germacrone exerts anti-cancer effects on gastric cancer through induction of cell cycle arrest and promotion of apoptosisLei Wu0Lifen Wang1Xiangguo Tian2Junyong Zhang3Hua Feng4Department of Digestive Disease, Shandong Provincial Hospital Affiliated to Shandong UniversityDepartment of Digestive Disease, Shandong Provincial Hospital Affiliated to Shandong UniversityDepartment of Digestive Disease, Shandong Provincial Hospital Affiliated to Shandong UniversityDepartment of Digestive Disease, Shandong Provincial Hospital Affiliated to Shandong UniversityDepartment of Digestive Disease, Shandong Provincial Hospital Affiliated to Shandong UniversityAbstract Background Germacrone is one of the natural bioactive compounds found in Rhizoma curcuma essential oils. In this study, the potential anti-cancer effect of germacrone in gastric cancer cell line BGC823 was investigated. Methods The cell viability and proliferative activity were assessed, and cell cycle analysis was also performed. Hoechst 33258 and Annexin V/PI double staining was used for detection of cell apoptosis. Protein profiles of cell cycle-related and apoptosis-related proteins were assessed. Results MTT assay revealed that germacrone had marked cytotoxicity on BGC823 cells. Germacrone induced cell cycle arrest in the G2/M phase via remarkably decreased expression levels of cyclin B1, cdc 2 and cdc 25c. In addition, the treatment with germacrone induced caspase-3 activity and PARP cleavage. These findings demonstrated the effects of germacrone on inhibiting cell proliferation through induction of G2/M phase cell cycle arrest and promotion of cell apoptosis. It also indicated that germacrone functioned through modulations of cell cycle-associated protein expression and mitochondria-mediated apoptosis. Conclusion These findings will be valuable as the molecular basis for the germacrone-mediated anti-cancer effect against gastric cancer.https://doi.org/10.1186/s12906-019-2810-3GermacroneCell cycle arrestApoptosisGastric cancerBGC823 cells |
| spellingShingle | Lei Wu Lifen Wang Xiangguo Tian Junyong Zhang Hua Feng Germacrone exerts anti-cancer effects on gastric cancer through induction of cell cycle arrest and promotion of apoptosis BMC Complementary Medicine and Therapies Germacrone Cell cycle arrest Apoptosis Gastric cancer BGC823 cells |
| title | Germacrone exerts anti-cancer effects on gastric cancer through induction of cell cycle arrest and promotion of apoptosis |
| title_full | Germacrone exerts anti-cancer effects on gastric cancer through induction of cell cycle arrest and promotion of apoptosis |
| title_fullStr | Germacrone exerts anti-cancer effects on gastric cancer through induction of cell cycle arrest and promotion of apoptosis |
| title_full_unstemmed | Germacrone exerts anti-cancer effects on gastric cancer through induction of cell cycle arrest and promotion of apoptosis |
| title_short | Germacrone exerts anti-cancer effects on gastric cancer through induction of cell cycle arrest and promotion of apoptosis |
| title_sort | germacrone exerts anti cancer effects on gastric cancer through induction of cell cycle arrest and promotion of apoptosis |
| topic | Germacrone Cell cycle arrest Apoptosis Gastric cancer BGC823 cells |
| url | https://doi.org/10.1186/s12906-019-2810-3 |
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