Bioactivity of Trichoderma harzianum A peptaibols against Zymoseptoria tritici causal agent of septoria leaf blotch of wheat
In this study Trichoderma harzianum strain A was isolated from the rhizosphere of an argan tree in southern Morocco. Trichoderma harzianum strain A had previously demonstrated a high antagonistic potential in vitro by direct confrontation and in vivo on wheat plants in pots under greenhouse conditio...
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| Format: | Article |
| Language: | English |
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Polish Academy of Sciences
2023-03-01
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| Series: | Journal of Plant Protection Research |
| Subjects: | |
| Online Access: | https://journals.pan.pl/Content/126702/PDF/OA_06_JPPR_63_1_1487_Barakat.pdf |
| _version_ | 1797862370600550400 |
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| author | Ilham Barakat Noureddine Chtaina Taha El Kamli Philippe Grappin Mohammed El Guilli Brahim Ezzahiri |
| author_facet | Ilham Barakat Noureddine Chtaina Taha El Kamli Philippe Grappin Mohammed El Guilli Brahim Ezzahiri |
| author_sort | Ilham Barakat |
| collection | DOAJ |
| description | In this study Trichoderma harzianum strain A was isolated from the rhizosphere of an argan tree in southern Morocco. Trichoderma harzianum strain A had previously demonstrated a high antagonistic potential in vitro by direct confrontation and in vivo on wheat plants in pots under greenhouse conditions against Zymoseptoria tritici, the agent of septoria leaf blotch. In this study, the activity of filtrates prepared from the liquid culture of T. harzianum A alone and from the confrontation medium with two Z. tritici strains [G1-1 (durum wheat) and A5-1 (soft wheat)] on the inhibition of Z. tritici pycnidiospore germination was studied by nephelometry. The results of the antibiosis assay revealed that filtrate 0 (A in confrontation with G1-1) and F3 (A against A5-1) showed 95% of G1-1 and A5-1 pycnidiospore inhibition at 9/10 dilution of the undiluted filtrates after 4 days of incubation. To understand and explain the antifungal activity of these filtrates, the extraction and identification of secondary molecules of peptaibiotic nature secreted by T. harzianum A in the three studied filtrates were performed. According to the results of high-performance liquid chromatography-mass spectrometry (HPLC-MS) analyses, 38 peptaibiotic molecules reported in the literature for their antifungal activity were identified in the different extracts at high concentrations (high peak intensities). These molecules are divided into nine groups, namely: Trichocryptin, Trichobrevin, Triochocryptin, Hypocompactin, Hyporodicin, Trichocompactin, Alamethicine, Trichoferin, and Trichokonin. It was also shown that the presence of the pathogen induces the production of peptaibols by the antagonistic strain of Trichoderma. |
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| format | Article |
| id | doaj.art-4f3097c4ed85447cbc55993f09d8911a |
| institution | Directory Open Access Journal |
| issn | 1427-4345 1899-007X |
| language | English |
| last_indexed | 2024-04-09T22:19:19Z |
| publishDate | 2023-03-01 |
| publisher | Polish Academy of Sciences |
| record_format | Article |
| series | Journal of Plant Protection Research |
| spelling | doaj.art-4f3097c4ed85447cbc55993f09d8911a2023-03-22T17:29:37ZengPolish Academy of SciencesJournal of Plant Protection Research1427-43451899-007X2023-03-01vol. 63No 15967https://doi.org/10.24425/jppr.2023.144504Bioactivity of Trichoderma harzianum A peptaibols against Zymoseptoria tritici causal agent of septoria leaf blotch of wheatIlham Barakat0https://orcid.org/0000-0002-8753-3963Noureddine Chtaina1Taha El Kamli2Philippe Grappin3Mohammed El Guilli4Brahim Ezzahiri5Department of Production, Protection, and Biotechnology, Hassan II Institute of Agronomic and Veterinary Medicine, Rabat, MoroccoDepartment of Production, Protection, and Biotechnology, Hassan II Institute of Agronomic and Veterinary Medicine, Rabat, MoroccoDepartment of Veterinary, Hassan II Institute of Agronomic and Veterinary Medicine, Rabat, MoroccoFungiSem, IRHS, INRA-University of Angers-Agrocampus-Ouest, Angers, FranceLaboratory of Phytopathology and Post-Harvest Quality, Regional Centre for Agronomic Research, Kénitra, MoroccoDepartment of Production, Protection, and Biotechnology, Hassan II Institute of Agronomic and Veterinary Medicine, Rabat, MoroccoIn this study Trichoderma harzianum strain A was isolated from the rhizosphere of an argan tree in southern Morocco. Trichoderma harzianum strain A had previously demonstrated a high antagonistic potential in vitro by direct confrontation and in vivo on wheat plants in pots under greenhouse conditions against Zymoseptoria tritici, the agent of septoria leaf blotch. In this study, the activity of filtrates prepared from the liquid culture of T. harzianum A alone and from the confrontation medium with two Z. tritici strains [G1-1 (durum wheat) and A5-1 (soft wheat)] on the inhibition of Z. tritici pycnidiospore germination was studied by nephelometry. The results of the antibiosis assay revealed that filtrate 0 (A in confrontation with G1-1) and F3 (A against A5-1) showed 95% of G1-1 and A5-1 pycnidiospore inhibition at 9/10 dilution of the undiluted filtrates after 4 days of incubation. To understand and explain the antifungal activity of these filtrates, the extraction and identification of secondary molecules of peptaibiotic nature secreted by T. harzianum A in the three studied filtrates were performed. According to the results of high-performance liquid chromatography-mass spectrometry (HPLC-MS) analyses, 38 peptaibiotic molecules reported in the literature for their antifungal activity were identified in the different extracts at high concentrations (high peak intensities). These molecules are divided into nine groups, namely: Trichocryptin, Trichobrevin, Triochocryptin, Hypocompactin, Hyporodicin, Trichocompactin, Alamethicine, Trichoferin, and Trichokonin. It was also shown that the presence of the pathogen induces the production of peptaibols by the antagonistic strain of Trichoderma.https://journals.pan.pl/Content/126702/PDF/OA_06_JPPR_63_1_1487_Barakat.pdfantibiosisnephelometrypeptaibolstrichoderma harzianumzymoseptoria tritici |
| spellingShingle | Ilham Barakat Noureddine Chtaina Taha El Kamli Philippe Grappin Mohammed El Guilli Brahim Ezzahiri Bioactivity of Trichoderma harzianum A peptaibols against Zymoseptoria tritici causal agent of septoria leaf blotch of wheat Journal of Plant Protection Research antibiosis nephelometry peptaibols trichoderma harzianum zymoseptoria tritici |
| title | Bioactivity of Trichoderma harzianum A peptaibols against Zymoseptoria tritici causal agent of septoria leaf blotch of wheat |
| title_full | Bioactivity of Trichoderma harzianum A peptaibols against Zymoseptoria tritici causal agent of septoria leaf blotch of wheat |
| title_fullStr | Bioactivity of Trichoderma harzianum A peptaibols against Zymoseptoria tritici causal agent of septoria leaf blotch of wheat |
| title_full_unstemmed | Bioactivity of Trichoderma harzianum A peptaibols against Zymoseptoria tritici causal agent of septoria leaf blotch of wheat |
| title_short | Bioactivity of Trichoderma harzianum A peptaibols against Zymoseptoria tritici causal agent of septoria leaf blotch of wheat |
| title_sort | bioactivity of trichoderma harzianum a peptaibols against zymoseptoria tritici causal agent of septoria leaf blotch of wheat |
| topic | antibiosis nephelometry peptaibols trichoderma harzianum zymoseptoria tritici |
| url | https://journals.pan.pl/Content/126702/PDF/OA_06_JPPR_63_1_1487_Barakat.pdf |
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