Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S
Endoplasmic reticulum (ER) stress, which can be induced by reactive oxygen species (ROS) and multiple factors, is associated with numerous intestinal diseases. The organic selenium source 2-hydroxy-4-methylselenobutanoic acid (HMSeBA), has been proved to decrease intestinal inflammation and autophag...
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| Format: | Article |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.900421/full |
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| author | Dajiang Ding Dajiang Ding Daolin Mou Daolin Mou Daolin Mou Daolin Mou Heng Zhu Heng Zhu Xuemei Jiang Xuemei Jiang Lianqiang Che Lianqiang Che Zhengfeng Fang Zhengfeng Fang Shengyu Xu Shengyu Xu Yan Lin Yan Lin Yong Zhuo Yong Zhuo Jian Li Jian Li Jian Li Chao Huang Yuanfeng Zou Lixia Li De Wu De Wu De Wu Bin Feng Bin Feng Bin Feng |
| author_facet | Dajiang Ding Dajiang Ding Daolin Mou Daolin Mou Daolin Mou Daolin Mou Heng Zhu Heng Zhu Xuemei Jiang Xuemei Jiang Lianqiang Che Lianqiang Che Zhengfeng Fang Zhengfeng Fang Shengyu Xu Shengyu Xu Yan Lin Yan Lin Yong Zhuo Yong Zhuo Jian Li Jian Li Jian Li Chao Huang Yuanfeng Zou Lixia Li De Wu De Wu De Wu Bin Feng Bin Feng Bin Feng |
| author_sort | Dajiang Ding |
| collection | DOAJ |
| description | Endoplasmic reticulum (ER) stress, which can be induced by reactive oxygen species (ROS) and multiple factors, is associated with numerous intestinal diseases. The organic selenium source 2-hydroxy-4-methylselenobutanoic acid (HMSeBA), has been proved to decrease intestinal inflammation and autophagy by improving the expression of selenoproteins. However, it remains unclear whether HMSeBA could alleviate intestinal ER stress by decreasing excessive production of ROS products. This study was conducted to investigate the effect of maternal HMSeBA supplementation on the regulation of intestinal ER stress of their offspring and the regulatory mechanism. Sows were supplemented with HMSeBA during gestation and jejunal epithelial (IPEC-J2) cells were treatment with HMSeBA. Results showed that maternal HMSeBA supplementation significantly upregulated mRNA level of selenoprotein S (SELS) in the jejunum of newborn and weaned piglets compared with the control group, while decreased the gene expression and protein abundance of ER stress markers in the jejunum of LPS challenged weaned piglets. In addition, HMSeBA treatment significantly increased the expression of glutathione peroxidase 4 (GPX4) and SELS, while decreased ROS level and the expression of ER stress markers induced by hydrogen peroxide (H2O2) in IPEC-J2 cells. Furthermore, knockdown of GPX4 did not enhance the ERS signal induced by H2O2, but the lack of GPX4 would cause further deterioration of ER stress signal in the absence of SELS. In conclusion, maternal HMSeBA supplementation might alleviate ROS induced intestinal ER stress by improving the expression of SELS and GPX4 in their offspring. Thus, maternal HMSeBA supplementation might be benefit for the intestinal health of their offspring. |
| first_indexed | 2024-12-12T04:02:58Z |
| format | Article |
| id | doaj.art-4f33f8b9ca554741ad49b0c36c38c4bd |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-12T04:02:58Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-4f33f8b9ca554741ad49b0c36c38c4bd2022-12-22T00:38:52ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-05-01910.3389/fnut.2022.900421900421Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein SDajiang Ding0Dajiang Ding1Daolin Mou2Daolin Mou3Daolin Mou4Daolin Mou5Heng Zhu6Heng Zhu7Xuemei Jiang8Xuemei Jiang9Lianqiang Che10Lianqiang Che11Zhengfeng Fang12Zhengfeng Fang13Shengyu Xu14Shengyu Xu15Yan Lin16Yan Lin17Yong Zhuo18Yong Zhuo19Jian Li20Jian Li21Jian Li22Chao Huang23Yuanfeng Zou24Lixia Li25De Wu26De Wu27De Wu28Bin Feng29Bin Feng30Bin Feng31Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaCollege of Bioengineering, Sichuan University of Science and Engineering, Zigong, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaCollege of Veterinary Medicine, Sichuan Agricultural University, Chengdu, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaAnimal Nutrition Institute, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Ministry of Education, Sichuan Agricultural University, Chengdu, ChinaKey Laboratory of Animal Disease-Resistant Nutrition of Sichuan Province, Sichuan Agricultural University, Chengdu, ChinaEndoplasmic reticulum (ER) stress, which can be induced by reactive oxygen species (ROS) and multiple factors, is associated with numerous intestinal diseases. The organic selenium source 2-hydroxy-4-methylselenobutanoic acid (HMSeBA), has been proved to decrease intestinal inflammation and autophagy by improving the expression of selenoproteins. However, it remains unclear whether HMSeBA could alleviate intestinal ER stress by decreasing excessive production of ROS products. This study was conducted to investigate the effect of maternal HMSeBA supplementation on the regulation of intestinal ER stress of their offspring and the regulatory mechanism. Sows were supplemented with HMSeBA during gestation and jejunal epithelial (IPEC-J2) cells were treatment with HMSeBA. Results showed that maternal HMSeBA supplementation significantly upregulated mRNA level of selenoprotein S (SELS) in the jejunum of newborn and weaned piglets compared with the control group, while decreased the gene expression and protein abundance of ER stress markers in the jejunum of LPS challenged weaned piglets. In addition, HMSeBA treatment significantly increased the expression of glutathione peroxidase 4 (GPX4) and SELS, while decreased ROS level and the expression of ER stress markers induced by hydrogen peroxide (H2O2) in IPEC-J2 cells. Furthermore, knockdown of GPX4 did not enhance the ERS signal induced by H2O2, but the lack of GPX4 would cause further deterioration of ER stress signal in the absence of SELS. In conclusion, maternal HMSeBA supplementation might alleviate ROS induced intestinal ER stress by improving the expression of SELS and GPX4 in their offspring. Thus, maternal HMSeBA supplementation might be benefit for the intestinal health of their offspring.https://www.frontiersin.org/articles/10.3389/fnut.2022.900421/fullseleniumER stressselenoprotein SGPX4intestine |
| spellingShingle | Dajiang Ding Dajiang Ding Daolin Mou Daolin Mou Daolin Mou Daolin Mou Heng Zhu Heng Zhu Xuemei Jiang Xuemei Jiang Lianqiang Che Lianqiang Che Zhengfeng Fang Zhengfeng Fang Shengyu Xu Shengyu Xu Yan Lin Yan Lin Yong Zhuo Yong Zhuo Jian Li Jian Li Jian Li Chao Huang Yuanfeng Zou Lixia Li De Wu De Wu De Wu Bin Feng Bin Feng Bin Feng Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S Frontiers in Nutrition selenium ER stress selenoprotein S GPX4 intestine |
| title | Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S |
| title_full | Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S |
| title_fullStr | Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S |
| title_full_unstemmed | Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S |
| title_short | Maternal Organic Selenium Supplementation Relieves Intestinal Endoplasmic Reticulum Stress in Piglets by Enhancing the Expression of Glutathione Peroxidase 4 and Selenoprotein S |
| title_sort | maternal organic selenium supplementation relieves intestinal endoplasmic reticulum stress in piglets by enhancing the expression of glutathione peroxidase 4 and selenoprotein s |
| topic | selenium ER stress selenoprotein S GPX4 intestine |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.900421/full |
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