Recent insights into the structure and function of coronavirus ribonucleases
Coronaviruses use approximately two‐thirds of their 30‐kb genomes to encode nonstructural proteins (nsps) with diverse functions that assist in viral replication and transcription, and evasion of the host immune response. The SARS‐CoV‐2 pandemic has led to renewed interest in the molecular mechanism...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-09-01
|
| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.13414 |
| _version_ | 1828120954538033152 |
|---|---|
| author | Meredith N. Frazier Amanda A. Riccio Isha M. Wilson William C. Copeland Robin E. Stanley |
| author_facet | Meredith N. Frazier Amanda A. Riccio Isha M. Wilson William C. Copeland Robin E. Stanley |
| author_sort | Meredith N. Frazier |
| collection | DOAJ |
| description | Coronaviruses use approximately two‐thirds of their 30‐kb genomes to encode nonstructural proteins (nsps) with diverse functions that assist in viral replication and transcription, and evasion of the host immune response. The SARS‐CoV‐2 pandemic has led to renewed interest in the molecular mechanisms used by coronaviruses to infect cells and replicate. Among the 16 Nsps involved in replication and transcription, coronaviruses encode two ribonucleases that process the viral RNA—an exonuclease (Nsp14) and an endonuclease (Nsp15). In this review, we discuss recent structural and biochemical studies of these nucleases and the implications for drug discovery. |
| first_indexed | 2024-04-11T14:15:44Z |
| format | Article |
| id | doaj.art-4f34b159ad4f4aff82ea99ddef73b102 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-11T14:15:44Z |
| publishDate | 2022-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-4f34b159ad4f4aff82ea99ddef73b1022022-12-22T04:19:33ZengWileyFEBS Open Bio2211-54632022-09-011291567158310.1002/2211-5463.13414Recent insights into the structure and function of coronavirus ribonucleasesMeredith N. Frazier0Amanda A. Riccio1Isha M. Wilson2William C. Copeland3Robin E. Stanley4Signal Transduction Laboratory Department of Health and Human Services National Institute of Environmental Health Sciences National Institutes of Health Research Triangle Park NC USAGenome Integrity and Structural Biology Laboratory Department of Health and Human Services National Institute of Environmental Health Sciences National Institutes of Health Research Triangle Park NC USASignal Transduction Laboratory Department of Health and Human Services National Institute of Environmental Health Sciences National Institutes of Health Research Triangle Park NC USAGenome Integrity and Structural Biology Laboratory Department of Health and Human Services National Institute of Environmental Health Sciences National Institutes of Health Research Triangle Park NC USASignal Transduction Laboratory Department of Health and Human Services National Institute of Environmental Health Sciences National Institutes of Health Research Triangle Park NC USACoronaviruses use approximately two‐thirds of their 30‐kb genomes to encode nonstructural proteins (nsps) with diverse functions that assist in viral replication and transcription, and evasion of the host immune response. The SARS‐CoV‐2 pandemic has led to renewed interest in the molecular mechanisms used by coronaviruses to infect cells and replicate. Among the 16 Nsps involved in replication and transcription, coronaviruses encode two ribonucleases that process the viral RNA—an exonuclease (Nsp14) and an endonuclease (Nsp15). In this review, we discuss recent structural and biochemical studies of these nucleases and the implications for drug discovery.https://doi.org/10.1002/2211-5463.13414coronavirusesdrug designendonucleasesexonucleasesSARS‐CoV‐2structural biology |
| spellingShingle | Meredith N. Frazier Amanda A. Riccio Isha M. Wilson William C. Copeland Robin E. Stanley Recent insights into the structure and function of coronavirus ribonucleases FEBS Open Bio coronaviruses drug design endonucleases exonucleases SARS‐CoV‐2 structural biology |
| title | Recent insights into the structure and function of coronavirus ribonucleases |
| title_full | Recent insights into the structure and function of coronavirus ribonucleases |
| title_fullStr | Recent insights into the structure and function of coronavirus ribonucleases |
| title_full_unstemmed | Recent insights into the structure and function of coronavirus ribonucleases |
| title_short | Recent insights into the structure and function of coronavirus ribonucleases |
| title_sort | recent insights into the structure and function of coronavirus ribonucleases |
| topic | coronaviruses drug design endonucleases exonucleases SARS‐CoV‐2 structural biology |
| url | https://doi.org/10.1002/2211-5463.13414 |
| work_keys_str_mv | AT meredithnfrazier recentinsightsintothestructureandfunctionofcoronavirusribonucleases AT amandaariccio recentinsightsintothestructureandfunctionofcoronavirusribonucleases AT ishamwilson recentinsightsintothestructureandfunctionofcoronavirusribonucleases AT williamccopeland recentinsightsintothestructureandfunctionofcoronavirusribonucleases AT robinestanley recentinsightsintothestructureandfunctionofcoronavirusribonucleases |