Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays
Abutilon hirtum (Lam.) Sweet belongs to the Malvaceae family and is a perennial shrub commonly known as Indian mallow, which is distributed in tropical regions and many different areas in Saudi Arabia. A. hirtum is an economically and a medicinally useful plant in different zones of the world for he...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023058255 |
| _version_ | 1827856215031414784 |
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| author | Sameer H. Qari |
| author_facet | Sameer H. Qari |
| author_sort | Sameer H. Qari |
| collection | DOAJ |
| description | Abutilon hirtum (Lam.) Sweet belongs to the Malvaceae family and is a perennial shrub commonly known as Indian mallow, which is distributed in tropical regions and many different areas in Saudi Arabia. A. hirtum is an economically and a medicinally useful plant in different zones of the world for healing various diseases. The current research explores the in vitro antioxidant characteristics and cytotoxic and genotoxic potential effects of the ethanolic leaf extract of A. hirtum (LEAH). Mitotic index (MI), micronucleus (MN), and chromosomal aberration (CA) tests were performed in Allium cepa, and MTT assays were performed using human dermal fibroblast, adult (HDFa) and breast cancer (MCF7) cell lines. The comet assay was used to assess the genotoxic effect of LEAH. The antioxidant activity of LEAH was evaluated by DPPH and superoxide anion free radical scavenging assays. The results revealed that the cytotoxic effects of LEAH on Allium cepa were significantly changed in an inverse relationship with MI (general average for 3 times 0.275) and a direct relationship with MNs and CAs against concentration and treatment time, for highest concentration 1.351 mg/ml were 0.74 and 1.81 respectively. In addition, the MTT test revealed a dose-dependent cytotoxic impact, with the % cell viability decreasing as the concentration of LEAH increased, the lowest % cell viability (19.54) and highest inhibition (80.46) were obtained with 4.40 mg/ml of LEAH. In general, the results indicated that A. hirtum has a damaging effect at high doses; however, a cell safe effect, and a strong antioxidant and DNA protective effect at carefully calculated doses was observed. This provides credibility to justify its general therapeutic activity. Moreover, future studies should identify bioactive molecules and their molecular mechanisms responsible for potential therapies. |
| first_indexed | 2024-03-12T12:22:30Z |
| format | Article |
| id | doaj.art-4f3581d69856487ba673e19a57c198e4 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-12T12:22:30Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-4f3581d69856487ba673e19a57c198e42023-08-30T05:51:57ZengElsevierHeliyon2405-84402023-08-0198e18617Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assaysSameer H. Qari0Department of Biology, Genetics and Molecular Biology Central Laboratory, Aljumum University College, Umm Al-Qura University, Makkah, Saudi ArabiaAbutilon hirtum (Lam.) Sweet belongs to the Malvaceae family and is a perennial shrub commonly known as Indian mallow, which is distributed in tropical regions and many different areas in Saudi Arabia. A. hirtum is an economically and a medicinally useful plant in different zones of the world for healing various diseases. The current research explores the in vitro antioxidant characteristics and cytotoxic and genotoxic potential effects of the ethanolic leaf extract of A. hirtum (LEAH). Mitotic index (MI), micronucleus (MN), and chromosomal aberration (CA) tests were performed in Allium cepa, and MTT assays were performed using human dermal fibroblast, adult (HDFa) and breast cancer (MCF7) cell lines. The comet assay was used to assess the genotoxic effect of LEAH. The antioxidant activity of LEAH was evaluated by DPPH and superoxide anion free radical scavenging assays. The results revealed that the cytotoxic effects of LEAH on Allium cepa were significantly changed in an inverse relationship with MI (general average for 3 times 0.275) and a direct relationship with MNs and CAs against concentration and treatment time, for highest concentration 1.351 mg/ml were 0.74 and 1.81 respectively. In addition, the MTT test revealed a dose-dependent cytotoxic impact, with the % cell viability decreasing as the concentration of LEAH increased, the lowest % cell viability (19.54) and highest inhibition (80.46) were obtained with 4.40 mg/ml of LEAH. In general, the results indicated that A. hirtum has a damaging effect at high doses; however, a cell safe effect, and a strong antioxidant and DNA protective effect at carefully calculated doses was observed. This provides credibility to justify its general therapeutic activity. Moreover, future studies should identify bioactive molecules and their molecular mechanisms responsible for potential therapies.http://www.sciencedirect.com/science/article/pii/S2405844023058255Abutilon hirtumGenotoxicityAntioxidantCytotoxicityEthnomedicine |
| spellingShingle | Sameer H. Qari Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays Heliyon Abutilon hirtum Genotoxicity Antioxidant Cytotoxicity Ethnomedicine |
| title | Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays |
| title_full | Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays |
| title_fullStr | Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays |
| title_full_unstemmed | Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays |
| title_short | Evaluation of the antioxidant activity, genotoxic, and cytotoxic effects of the ethanolic leaves extract of Abutilon hirtum (Lam.) Sweet using in vitro assays |
| title_sort | evaluation of the antioxidant activity genotoxic and cytotoxic effects of the ethanolic leaves extract of abutilon hirtum lam sweet using in vitro assays |
| topic | Abutilon hirtum Genotoxicity Antioxidant Cytotoxicity Ethnomedicine |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023058255 |
| work_keys_str_mv | AT sameerhqari evaluationoftheantioxidantactivitygenotoxicandcytotoxiceffectsoftheethanolicleavesextractofabutilonhirtumlamsweetusinginvitroassays |