An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7.
BACKGROUND: An aptamer based biosensor (aptasensor) was developed and evaluated for rapid colorimetric detection of Escherichia coli (E. coli) O157:H7. METHODOLOGY/PRINCIPAL FINDINGS: The aptasensor was assembled by modifying the truncated lipopolysaccharides (LPS)-binding aptamer on the surface of...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3492178?pdf=render |
| _version_ | 1819127929352749056 |
|---|---|
| author | Wenhe Wu Jie Zhang Meiqin Zheng Yuhong Zhong Jie Yang Yuhong Zhao Wenping Wu Wei Ye Jie Wen Qi Wang Jianxin Lu |
| author_facet | Wenhe Wu Jie Zhang Meiqin Zheng Yuhong Zhong Jie Yang Yuhong Zhao Wenping Wu Wei Ye Jie Wen Qi Wang Jianxin Lu |
| author_sort | Wenhe Wu |
| collection | DOAJ |
| description | BACKGROUND: An aptamer based biosensor (aptasensor) was developed and evaluated for rapid colorimetric detection of Escherichia coli (E. coli) O157:H7. METHODOLOGY/PRINCIPAL FINDINGS: The aptasensor was assembled by modifying the truncated lipopolysaccharides (LPS)-binding aptamer on the surface of nanoscale polydiacetylene (PDA) vesicle using peptide bonding between the carboxyl group of the vesicle and the amine group of the aptamer. Molecular recognition between E. coli O157:H7 and aptamer at the interface of the vesicle lead to blue-red transition of PDA which was readily visible to the naked eyes and could be quantified by colorimetric responses (CR). Confocal laser scanning microscope (CLSM) and transmission electron microscopy (TEM) was used to confirm the specific interactions between the truncated aptamer and E. coli O157:H7. The aptasensor could detect cellular concentrations in a range of 10(4)~ 10(8) colony-forming units (CFU)/ml within 2 hours and its specificity was 100% for detection of E. coli O157:H7. Compared with the standard culture method, the correspondent rate was 98.5% for the detection of E. coli O157:H7 on 203 clinical fecal specimens with our aptasensor. CONCLUSIONS: The new aptasensor represents a significant advancement in detection capabilities based on the combination of nucleic acid aptamer with PDA vesicle, and offers a specific and convenient screening method for the detection of pathogenic bacteria. This technic could also be applied in areas from clinical analysis to biological terrorism defense, especially in low-resource settings. |
| first_indexed | 2024-12-22T08:19:44Z |
| format | Article |
| id | doaj.art-4f3b1af1d64a482591513cb551536baf |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T08:19:44Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4f3b1af1d64a482591513cb551536baf2022-12-21T18:32:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e4899910.1371/journal.pone.0048999An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7.Wenhe WuJie ZhangMeiqin ZhengYuhong ZhongJie YangYuhong ZhaoWenping WuWei YeJie WenQi WangJianxin LuBACKGROUND: An aptamer based biosensor (aptasensor) was developed and evaluated for rapid colorimetric detection of Escherichia coli (E. coli) O157:H7. METHODOLOGY/PRINCIPAL FINDINGS: The aptasensor was assembled by modifying the truncated lipopolysaccharides (LPS)-binding aptamer on the surface of nanoscale polydiacetylene (PDA) vesicle using peptide bonding between the carboxyl group of the vesicle and the amine group of the aptamer. Molecular recognition between E. coli O157:H7 and aptamer at the interface of the vesicle lead to blue-red transition of PDA which was readily visible to the naked eyes and could be quantified by colorimetric responses (CR). Confocal laser scanning microscope (CLSM) and transmission electron microscopy (TEM) was used to confirm the specific interactions between the truncated aptamer and E. coli O157:H7. The aptasensor could detect cellular concentrations in a range of 10(4)~ 10(8) colony-forming units (CFU)/ml within 2 hours and its specificity was 100% for detection of E. coli O157:H7. Compared with the standard culture method, the correspondent rate was 98.5% for the detection of E. coli O157:H7 on 203 clinical fecal specimens with our aptasensor. CONCLUSIONS: The new aptasensor represents a significant advancement in detection capabilities based on the combination of nucleic acid aptamer with PDA vesicle, and offers a specific and convenient screening method for the detection of pathogenic bacteria. This technic could also be applied in areas from clinical analysis to biological terrorism defense, especially in low-resource settings.http://europepmc.org/articles/PMC3492178?pdf=render |
| spellingShingle | Wenhe Wu Jie Zhang Meiqin Zheng Yuhong Zhong Jie Yang Yuhong Zhao Wenping Wu Wei Ye Jie Wen Qi Wang Jianxin Lu An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7. PLoS ONE |
| title | An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7. |
| title_full | An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7. |
| title_fullStr | An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7. |
| title_full_unstemmed | An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7. |
| title_short | An aptamer-based biosensor for colorimetric detection of Escherichia coli O157:H7. |
| title_sort | aptamer based biosensor for colorimetric detection of escherichia coli o157 h7 |
| url | http://europepmc.org/articles/PMC3492178?pdf=render |
| work_keys_str_mv | AT wenhewu anaptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT jiezhang anaptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT meiqinzheng anaptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT yuhongzhong anaptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT jieyang anaptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT yuhongzhao anaptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT wenpingwu anaptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT weiye anaptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT jiewen anaptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT qiwang anaptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT jianxinlu anaptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT wenhewu aptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT jiezhang aptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT meiqinzheng aptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT yuhongzhong aptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT jieyang aptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT yuhongzhao aptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT wenpingwu aptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT weiye aptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT jiewen aptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT qiwang aptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 AT jianxinlu aptamerbasedbiosensorforcolorimetricdetectionofescherichiacolio157h7 |