Alternative Architecture of the <i>E. coli</i> Chemosensory Array
Chemotactic responses in motile bacteria are the result of sophisticated signal transduction by large, highly organized arrays of sensory proteins. Despite tremendous progress in the understanding of chemosensory array structure and function, a structural basis for the heightened sensitivity of netw...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/4/495 |
| _version_ | 1797540019814006784 |
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| author | Alister Burt C. Keith Cassidy Phillip J. Stansfeld Irina Gutsche |
| author_facet | Alister Burt C. Keith Cassidy Phillip J. Stansfeld Irina Gutsche |
| author_sort | Alister Burt |
| collection | DOAJ |
| description | Chemotactic responses in motile bacteria are the result of sophisticated signal transduction by large, highly organized arrays of sensory proteins. Despite tremendous progress in the understanding of chemosensory array structure and function, a structural basis for the heightened sensitivity of networked chemoreceptors is not yet complete. Here, we present cryo-electron tomography visualisations of native-state chemosensory arrays in <i>E. coli</i> minicells. Strikingly, these arrays appear to exhibit a p2-symmetric array architecture that differs markedly from the p6-symmetric architecture previously described in <i>E. coli</i>. Based on this data, we propose molecular models of this alternative architecture and the canonical p6-symmetric assembly. We evaluate our observations and each model in the context of previously published data, assessing the functional implications of an alternative architecture and effects for future studies. |
| first_indexed | 2024-03-10T12:55:02Z |
| format | Article |
| id | doaj.art-4f3c553041d14a02a5c8248525ba0a0a |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T12:55:02Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-4f3c553041d14a02a5c8248525ba0a0a2023-11-21T12:00:11ZengMDPI AGBiomolecules2218-273X2021-03-0111449510.3390/biom11040495Alternative Architecture of the <i>E. coli</i> Chemosensory ArrayAlister Burt0C. Keith Cassidy1Phillip J. Stansfeld2Irina Gutsche3Institut de Biologie Structurale, Université Grenoble Alpes, CEA, CNRS, IBS, 71 Avenue des Martyrs, F-38044 Grenoble, FranceDepartment of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, UKDepartment of Chemistry, School of Life Sciences, University of Warwick, Gibbet Hill Campus, Coventry CV4 7AL, UKInstitut de Biologie Structurale, Université Grenoble Alpes, CEA, CNRS, IBS, 71 Avenue des Martyrs, F-38044 Grenoble, FranceChemotactic responses in motile bacteria are the result of sophisticated signal transduction by large, highly organized arrays of sensory proteins. Despite tremendous progress in the understanding of chemosensory array structure and function, a structural basis for the heightened sensitivity of networked chemoreceptors is not yet complete. Here, we present cryo-electron tomography visualisations of native-state chemosensory arrays in <i>E. coli</i> minicells. Strikingly, these arrays appear to exhibit a p2-symmetric array architecture that differs markedly from the p6-symmetric architecture previously described in <i>E. coli</i>. Based on this data, we propose molecular models of this alternative architecture and the canonical p6-symmetric assembly. We evaluate our observations and each model in the context of previously published data, assessing the functional implications of an alternative architecture and effects for future studies.https://www.mdpi.com/2218-273X/11/4/495chemosensory arraychemotaxis<i>E. coli</i>cryo-electron tomographymolecular modelling |
| spellingShingle | Alister Burt C. Keith Cassidy Phillip J. Stansfeld Irina Gutsche Alternative Architecture of the <i>E. coli</i> Chemosensory Array Biomolecules chemosensory array chemotaxis <i>E. coli</i> cryo-electron tomography molecular modelling |
| title | Alternative Architecture of the <i>E. coli</i> Chemosensory Array |
| title_full | Alternative Architecture of the <i>E. coli</i> Chemosensory Array |
| title_fullStr | Alternative Architecture of the <i>E. coli</i> Chemosensory Array |
| title_full_unstemmed | Alternative Architecture of the <i>E. coli</i> Chemosensory Array |
| title_short | Alternative Architecture of the <i>E. coli</i> Chemosensory Array |
| title_sort | alternative architecture of the i e coli i chemosensory array |
| topic | chemosensory array chemotaxis <i>E. coli</i> cryo-electron tomography molecular modelling |
| url | https://www.mdpi.com/2218-273X/11/4/495 |
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