Expression of a Serratia marcescens Chitinase Gene in Sinorhizobium fredii USDA191 and Sinorhizobium meliloti RCR2011 Impedes Soybean and Alfalfa Nodulation
A gene encoding chitinase from Serratia marcescens BJL200 was cloned into a broad-host-range vector (pRK415) and mobilized into Sinorhizobium fredii USDA191. Chitinolytic activity was detected in S. fredii USDA191 transconjugants that carried the S. marcescens chiB gene. Chitinase-producing S. fredi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
1999-08-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.8.748 |
| _version_ | 1811278046979686400 |
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| author | Hari B. Krishnan Kil Yong Kim Ammulu Hari Krishnan |
| author_facet | Hari B. Krishnan Kil Yong Kim Ammulu Hari Krishnan |
| author_sort | Hari B. Krishnan |
| collection | DOAJ |
| description | A gene encoding chitinase from Serratia marcescens BJL200 was cloned into a broad-host-range vector (pRK415) and mobilized into Sinorhizobium fredii USDA191. Chitinolytic activity was detected in S. fredii USDA191 transconjugants that carried the S. marcescens chiB gene. Chitinase-producing S. fredii USDA191 formed nodules on soybean cultivar McCall. However, there was a delay in nodule formation and a marked decrease in the total number of nodules formed by the chitinase-producing S. fredii in comparison with the wild-type strain. Expression of chitinase in S. meliloti RCR2011 also impeded alfalfa nodulation. Thin-layer chromatography of 14C-labeled Nod factors from chitinase-producing S. fredii USDA191 revealed hydrolysis of lipochitooligosaccharides. |
| first_indexed | 2024-04-13T00:27:40Z |
| format | Article |
| id | doaj.art-4f3f42d67adc49fea2198f9cce8ca85e |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T00:27:40Z |
| publishDate | 1999-08-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-4f3f42d67adc49fea2198f9cce8ca85e2022-12-22T03:10:33ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77061999-08-0112874875110.1094/MPMI.1999.12.8.748Expression of a Serratia marcescens Chitinase Gene in Sinorhizobium fredii USDA191 and Sinorhizobium meliloti RCR2011 Impedes Soybean and Alfalfa NodulationHari B. KrishnanKil Yong KimAmmulu Hari KrishnanA gene encoding chitinase from Serratia marcescens BJL200 was cloned into a broad-host-range vector (pRK415) and mobilized into Sinorhizobium fredii USDA191. Chitinolytic activity was detected in S. fredii USDA191 transconjugants that carried the S. marcescens chiB gene. Chitinase-producing S. fredii USDA191 formed nodules on soybean cultivar McCall. However, there was a delay in nodule formation and a marked decrease in the total number of nodules formed by the chitinase-producing S. fredii in comparison with the wild-type strain. Expression of chitinase in S. meliloti RCR2011 also impeded alfalfa nodulation. Thin-layer chromatography of 14C-labeled Nod factors from chitinase-producing S. fredii USDA191 revealed hydrolysis of lipochitooligosaccharides.https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.8.748 |
| spellingShingle | Hari B. Krishnan Kil Yong Kim Ammulu Hari Krishnan Expression of a Serratia marcescens Chitinase Gene in Sinorhizobium fredii USDA191 and Sinorhizobium meliloti RCR2011 Impedes Soybean and Alfalfa Nodulation Molecular Plant-Microbe Interactions |
| title | Expression of a Serratia marcescens Chitinase Gene in Sinorhizobium fredii USDA191 and Sinorhizobium meliloti RCR2011 Impedes Soybean and Alfalfa Nodulation |
| title_full | Expression of a Serratia marcescens Chitinase Gene in Sinorhizobium fredii USDA191 and Sinorhizobium meliloti RCR2011 Impedes Soybean and Alfalfa Nodulation |
| title_fullStr | Expression of a Serratia marcescens Chitinase Gene in Sinorhizobium fredii USDA191 and Sinorhizobium meliloti RCR2011 Impedes Soybean and Alfalfa Nodulation |
| title_full_unstemmed | Expression of a Serratia marcescens Chitinase Gene in Sinorhizobium fredii USDA191 and Sinorhizobium meliloti RCR2011 Impedes Soybean and Alfalfa Nodulation |
| title_short | Expression of a Serratia marcescens Chitinase Gene in Sinorhizobium fredii USDA191 and Sinorhizobium meliloti RCR2011 Impedes Soybean and Alfalfa Nodulation |
| title_sort | expression of a serratia marcescens chitinase gene in sinorhizobium fredii usda191 and sinorhizobium meliloti rcr2011 impedes soybean and alfalfa nodulation |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.1999.12.8.748 |
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