Chemotyping of Fusarium graminearum using Tri13 trichothecene biosynthetic gene
Fusarium graminearum is one of the most important causes of FHB or wheat scab in different part of the world. This fungus is able to produce widespread Trichothecene mycotoxins such as Nivalenol (NIV) and Deoxynivalenol (DON) which are harmful for both human and animals. To determine chemotypes of T...
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| Format: | Article |
| Language: | English |
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University of Tarbiat Modares
2013-12-01
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| Series: | Journal of Crop Protection |
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| Online Access: | http://jcp.modares.ac.ir/article-3-5240-en.html |
| _version_ | 1828553956716969984 |
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| author | Mostafa Abedi-Tizaki Seyed Kazem Sabbagh Mahta Mazaheri Naeini Saedeh Sepehrikia |
| author_facet | Mostafa Abedi-Tizaki Seyed Kazem Sabbagh Mahta Mazaheri Naeini Saedeh Sepehrikia |
| author_sort | Mostafa Abedi-Tizaki |
| collection | DOAJ |
| description | Fusarium graminearum is one of the most important causes of FHB or wheat scab in different part of the world. This fungus is able to produce widespread Trichothecene mycotoxins such as Nivalenol (NIV) and Deoxynivalenol (DON) which are harmful for both human and animals. To determine chemotypes of Trichothecene, a total of 100 isolates from different fields of Golestan province in Iran including Gorgan, Kordkuy, Bandaregaz, Gonbad, Minodasht, Kalaleh and Azadshahr were identified as F. graminearum using morphological features then 96 isolates were confirmed by polymerase chain reaction (PCR) assay using F. graminearum species-specific primers (Fg16F/Fg16R). Based on sequences of Tri13 gene involved in the mycotoxin biosynthetic pathway, PCR assays was used to detect Nivalenol (NIV) and Deoxynivalenol (DON) chemotypes. Of the 96 tested isolates with Tri13 PCR assays, 70 classified as NIV chemotype and the remaining 26 isolates as DON producers. These results indicated that NIV chemotype was the most dominant chemotype in studied zones. A greater proportion of NIV chemotype was found in Gorgan fields (P < 0.05, P < 0.0001), whereas greater proportion of DON was detected in Gorgan and Gonbad fields (P < 0.05, P < 0.0001). Chemotyping by PCR assay were confirmed using HPLC method. These results demonstrated that PCR assay and HPLC could be used as rapid, reliable and cost-effective methods for the detection and identification of mycotoxin-producing Fusarium-species and may thus help to develop strategies to avoid or reduce mycotoxin contamination of cereals. |
| first_indexed | 2024-12-12T05:27:44Z |
| format | Article |
| id | doaj.art-4f3fa68c69af44fdabd7bed315b157c3 |
| institution | Directory Open Access Journal |
| issn | 2251-9041 2251-905X |
| language | English |
| last_indexed | 2024-12-12T05:27:44Z |
| publishDate | 2013-12-01 |
| publisher | University of Tarbiat Modares |
| record_format | Article |
| series | Journal of Crop Protection |
| spelling | doaj.art-4f3fa68c69af44fdabd7bed315b157c32022-12-22T00:36:25ZengUniversity of Tarbiat ModaresJournal of Crop Protection2251-90412251-905X2013-12-0124487500Chemotyping of Fusarium graminearum using Tri13 trichothecene biosynthetic geneMostafa Abedi-Tizaki0Seyed Kazem Sabbagh1Mahta Mazaheri Naeini2Saedeh Sepehrikia3 MSc Student of Plant Pathology, Department of Plant Pathology, University of Zabol, Zabol, Iran. Department of Plant Protection and Institute of Plant biotechnology, University of Zabol, Zabol, Iran. Faculty of Medicine, Zabol University of Medical Sciences, Zabol, Iran. Laboratory expert, Mashad University of Medical Sciences, Khorasan-Razavi, Iran. Fusarium graminearum is one of the most important causes of FHB or wheat scab in different part of the world. This fungus is able to produce widespread Trichothecene mycotoxins such as Nivalenol (NIV) and Deoxynivalenol (DON) which are harmful for both human and animals. To determine chemotypes of Trichothecene, a total of 100 isolates from different fields of Golestan province in Iran including Gorgan, Kordkuy, Bandaregaz, Gonbad, Minodasht, Kalaleh and Azadshahr were identified as F. graminearum using morphological features then 96 isolates were confirmed by polymerase chain reaction (PCR) assay using F. graminearum species-specific primers (Fg16F/Fg16R). Based on sequences of Tri13 gene involved in the mycotoxin biosynthetic pathway, PCR assays was used to detect Nivalenol (NIV) and Deoxynivalenol (DON) chemotypes. Of the 96 tested isolates with Tri13 PCR assays, 70 classified as NIV chemotype and the remaining 26 isolates as DON producers. These results indicated that NIV chemotype was the most dominant chemotype in studied zones. A greater proportion of NIV chemotype was found in Gorgan fields (P < 0.05, P < 0.0001), whereas greater proportion of DON was detected in Gorgan and Gonbad fields (P < 0.05, P < 0.0001). Chemotyping by PCR assay were confirmed using HPLC method. These results demonstrated that PCR assay and HPLC could be used as rapid, reliable and cost-effective methods for the detection and identification of mycotoxin-producing Fusarium-species and may thus help to develop strategies to avoid or reduce mycotoxin contamination of cereals.http://jcp.modares.ac.ir/article-3-5240-en.htmlfusarium graminearumtrichothecenechemotypesnivdon |
| spellingShingle | Mostafa Abedi-Tizaki Seyed Kazem Sabbagh Mahta Mazaheri Naeini Saedeh Sepehrikia Chemotyping of Fusarium graminearum using Tri13 trichothecene biosynthetic gene Journal of Crop Protection fusarium graminearum trichothecene chemotypes niv don |
| title | Chemotyping of Fusarium graminearum using Tri13 trichothecene biosynthetic gene |
| title_full | Chemotyping of Fusarium graminearum using Tri13 trichothecene biosynthetic gene |
| title_fullStr | Chemotyping of Fusarium graminearum using Tri13 trichothecene biosynthetic gene |
| title_full_unstemmed | Chemotyping of Fusarium graminearum using Tri13 trichothecene biosynthetic gene |
| title_short | Chemotyping of Fusarium graminearum using Tri13 trichothecene biosynthetic gene |
| title_sort | chemotyping of fusarium graminearum using tri13 trichothecene biosynthetic gene |
| topic | fusarium graminearum trichothecene chemotypes niv don |
| url | http://jcp.modares.ac.ir/article-3-5240-en.html |
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