Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor
Severe respiratory disease coronavirus-2 (SARS-CoV-2) has been the most devastating disease COVID-19 in the century. One of the unsolved scientific questions of SARS-CoV-2 is the animal origin of this virus. Bats and pangolins are recognized as the most probable reservoir hosts that harbour highly s...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2021.1956373 |
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| author | Hua Guo Ben Hu Hao-Rui Si Yan Zhu Wei Zhang Bei Li Ang Li Rong Geng Hao-Feng Lin Xing-Lou Yang Peng Zhou Zheng-Li Shi |
| author_facet | Hua Guo Ben Hu Hao-Rui Si Yan Zhu Wei Zhang Bei Li Ang Li Rong Geng Hao-Feng Lin Xing-Lou Yang Peng Zhou Zheng-Li Shi |
| author_sort | Hua Guo |
| collection | DOAJ |
| description | Severe respiratory disease coronavirus-2 (SARS-CoV-2) has been the most devastating disease COVID-19 in the century. One of the unsolved scientific questions of SARS-CoV-2 is the animal origin of this virus. Bats and pangolins are recognized as the most probable reservoir hosts that harbour highly similar SARS-CoV-2 related viruses (SARSr-CoV-2). This study identified a novel lineage of SARSr-CoVs, including RaTG15 and seven other viruses, from bats at the same location where we found RaTG13 in 2015. Although RaTG15 and the related viruses share 97.2% amino acid sequence identities with SARS-CoV-2 in the conserved ORF1b region, it only shows less than 77.6% nucleotide identity to all known SARSr-CoVs at the genome level, thus forming a distinct lineage in the Sarbecovirus phylogenetic tree. We found that the RaTG15 receptor-binding domain (RBD) can bind to ACE2 from Rhinolophus affinis, Malayan pangolin, and use it as an entry receptor, except for ACE2 from humans. However, it contains a short deletion and has different key residues responsible for ACE2 binding. In addition, we showed that none of the known viruses in bat SARSr-CoV-2 lineage discovered uses human ACE2 as efficiently as the pangolin-derived SARSr-CoV-2 or some viruses in the SARSr-CoV-1 lineage. Therefore, further systematic and longitudinal studies in bats are needed to prevent future spillover events caused by SARSr-CoVs or to understand the origin of SARS-CoV-2 better. |
| first_indexed | 2024-12-20T19:13:19Z |
| format | Article |
| id | doaj.art-4f4077eb64544fe7b8c355b7f237c486 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-12-20T19:13:19Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-4f4077eb64544fe7b8c355b7f237c4862022-12-21T19:29:10ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512021-01-011011507151410.1080/22221751.2021.1956373Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptorHua Guo0Ben Hu1Hao-Rui Si2Yan Zhu3Wei Zhang4Bei Li5Ang Li6Rong Geng7Hao-Feng Lin8Xing-Lou Yang9Peng Zhou10Zheng-Li Shi11CAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of ChinaCAS Key Laboratory of Special Pathogens and Biosafety, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan Institute of Virology, Wuhan, People’s Republic of ChinaSevere respiratory disease coronavirus-2 (SARS-CoV-2) has been the most devastating disease COVID-19 in the century. One of the unsolved scientific questions of SARS-CoV-2 is the animal origin of this virus. Bats and pangolins are recognized as the most probable reservoir hosts that harbour highly similar SARS-CoV-2 related viruses (SARSr-CoV-2). This study identified a novel lineage of SARSr-CoVs, including RaTG15 and seven other viruses, from bats at the same location where we found RaTG13 in 2015. Although RaTG15 and the related viruses share 97.2% amino acid sequence identities with SARS-CoV-2 in the conserved ORF1b region, it only shows less than 77.6% nucleotide identity to all known SARSr-CoVs at the genome level, thus forming a distinct lineage in the Sarbecovirus phylogenetic tree. We found that the RaTG15 receptor-binding domain (RBD) can bind to ACE2 from Rhinolophus affinis, Malayan pangolin, and use it as an entry receptor, except for ACE2 from humans. However, it contains a short deletion and has different key residues responsible for ACE2 binding. In addition, we showed that none of the known viruses in bat SARSr-CoV-2 lineage discovered uses human ACE2 as efficiently as the pangolin-derived SARSr-CoV-2 or some viruses in the SARSr-CoV-1 lineage. Therefore, further systematic and longitudinal studies in bats are needed to prevent future spillover events caused by SARSr-CoVs or to understand the origin of SARS-CoV-2 better.https://www.tandfonline.com/doi/10.1080/22221751.2021.1956373SARS-related coronavirusnovel lineagebatreservoir hostACE2 |
| spellingShingle | Hua Guo Ben Hu Hao-Rui Si Yan Zhu Wei Zhang Bei Li Ang Li Rong Geng Hao-Feng Lin Xing-Lou Yang Peng Zhou Zheng-Li Shi Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor Emerging Microbes and Infections SARS-related coronavirus novel lineage bat reservoir host ACE2 |
| title | Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor |
| title_full | Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor |
| title_fullStr | Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor |
| title_full_unstemmed | Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor |
| title_short | Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor |
| title_sort | identification of a novel lineage bat sars related coronaviruses that use bat ace2 receptor |
| topic | SARS-related coronavirus novel lineage bat reservoir host ACE2 |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2021.1956373 |
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