A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells
Abstract In the field of three‐dimensional (3D) cell culture and tissue engineering, great advance focusing on functionalized materials and desirable culture systems has been made to mimic the natural environment of cells in vivo. Mechanical loading is one of the critical factors that affect cell/ti...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-07-01
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| Series: | Advanced Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/advs.202003738 |
| _version_ | 1819143340535316480 |
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| author | Yu Qin Xue‐Bo Hu Wen‐Ting Fan Jing Yan Shi‐Bo Cheng Yan‐Ling Liu Wei‐Hua Huang |
| author_facet | Yu Qin Xue‐Bo Hu Wen‐Ting Fan Jing Yan Shi‐Bo Cheng Yan‐Ling Liu Wei‐Hua Huang |
| author_sort | Yu Qin |
| collection | DOAJ |
| description | Abstract In the field of three‐dimensional (3D) cell culture and tissue engineering, great advance focusing on functionalized materials and desirable culture systems has been made to mimic the natural environment of cells in vivo. Mechanical loading is one of the critical factors that affect cell/tissue behaviors and metabolic activities, but the reported models or detection methods offer little direct and real‐time information about mechanically induced cell responses. Herein, for the first time, a stretchable and multifunctional platform integrating 3D cell culture, mechanical loading, and electrochemical sensing is developed by immobilization of biomimetic peptide linked gold nanotubes on porous and elastic polydimethylsiloxane. The 3D scaffold demonstrates very good compatibility, excellent stretchability, and stable electrochemical sensing performance. This allows mimicking the articular cartilage and investigating its mechanotransduction by 3D culture, mechanical stretching of chondrocytes, and synchronously real‐time monitoring of stretch‐induced signaling molecules. The results disclose a previously unclear mechanotransduction pathway in chondrocytes that mechanical loading can rapidly activate nitric oxide signaling within seconds. This indicates the promising potential of the stretchable 3D sensing in exploring the mechanotransduction in 3D cellular systems and engineered tissues. |
| first_indexed | 2024-12-22T12:24:41Z |
| format | Article |
| id | doaj.art-4f445bf8664a456fba472547e36d5215 |
| institution | Directory Open Access Journal |
| issn | 2198-3844 |
| language | English |
| last_indexed | 2024-12-22T12:24:41Z |
| publishDate | 2021-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj.art-4f445bf8664a456fba472547e36d52152022-12-21T18:25:51ZengWileyAdvanced Science2198-38442021-07-01813n/an/a10.1002/advs.202003738A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of CellsYu Qin0Xue‐Bo Hu1Wen‐Ting Fan2Jing Yan3Shi‐Bo Cheng4Yan‐Ling Liu5Wei‐Hua Huang6College of Chemistry and Molecular Sciences Wuhan University Wuhan 430072 ChinaCollege of Chemistry and Chemical Engineering Institute for Conservation and Utilization of Agro‐Bioresources in Dabie Mountains Xinyang Normal University Xinyang 464000 ChinaCollege of Chemistry and Molecular Sciences Wuhan University Wuhan 430072 ChinaCollege of Chemistry and Molecular Sciences Wuhan University Wuhan 430072 ChinaCollege of Chemistry and Molecular Sciences Wuhan University Wuhan 430072 ChinaCollege of Chemistry and Molecular Sciences Wuhan University Wuhan 430072 ChinaCollege of Chemistry and Molecular Sciences Wuhan University Wuhan 430072 ChinaAbstract In the field of three‐dimensional (3D) cell culture and tissue engineering, great advance focusing on functionalized materials and desirable culture systems has been made to mimic the natural environment of cells in vivo. Mechanical loading is one of the critical factors that affect cell/tissue behaviors and metabolic activities, but the reported models or detection methods offer little direct and real‐time information about mechanically induced cell responses. Herein, for the first time, a stretchable and multifunctional platform integrating 3D cell culture, mechanical loading, and electrochemical sensing is developed by immobilization of biomimetic peptide linked gold nanotubes on porous and elastic polydimethylsiloxane. The 3D scaffold demonstrates very good compatibility, excellent stretchability, and stable electrochemical sensing performance. This allows mimicking the articular cartilage and investigating its mechanotransduction by 3D culture, mechanical stretching of chondrocytes, and synchronously real‐time monitoring of stretch‐induced signaling molecules. The results disclose a previously unclear mechanotransduction pathway in chondrocytes that mechanical loading can rapidly activate nitric oxide signaling within seconds. This indicates the promising potential of the stretchable 3D sensing in exploring the mechanotransduction in 3D cellular systems and engineered tissues.https://doi.org/10.1002/advs.2020037383D cell culturechondrocyteselectrochemical detectionmechanotransductionstretchable scaffolds |
| spellingShingle | Yu Qin Xue‐Bo Hu Wen‐Ting Fan Jing Yan Shi‐Bo Cheng Yan‐Ling Liu Wei‐Hua Huang A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells Advanced Science 3D cell culture chondrocytes electrochemical detection mechanotransduction stretchable scaffolds |
| title | A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells |
| title_full | A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells |
| title_fullStr | A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells |
| title_full_unstemmed | A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells |
| title_short | A Stretchable Scaffold with Electrochemical Sensing for 3D Culture, Mechanical Loading, and Real‐Time Monitoring of Cells |
| title_sort | stretchable scaffold with electrochemical sensing for 3d culture mechanical loading and real time monitoring of cells |
| topic | 3D cell culture chondrocytes electrochemical detection mechanotransduction stretchable scaffolds |
| url | https://doi.org/10.1002/advs.202003738 |
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