Low nitrogen status affects isoflavonoid production and flavonol decoration in Lotus corniculatus
Nitrogen availability is key factor for plant metabolism and has a strong impact on the biosynthesis of secondary plant metabolites. A number of these metabolites are involved in the response to stress. In this paper, we investigated the effect of different N conditions in leaves of Lotus corniculat...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Plant Stress |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667064X23002038 |
| _version_ | 1827390384279388160 |
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| author | Kristina Trush Martina Gavurová María Dolores Monje-Rueda Vladislav Kolarčik Michaela Bačovčinová Marco Betti Peter Paľove-Balang |
| author_facet | Kristina Trush Martina Gavurová María Dolores Monje-Rueda Vladislav Kolarčik Michaela Bačovčinová Marco Betti Peter Paľove-Balang |
| author_sort | Kristina Trush |
| collection | DOAJ |
| description | Nitrogen availability is key factor for plant metabolism and has a strong impact on the biosynthesis of secondary plant metabolites. A number of these metabolites are involved in the response to stress. In this paper, we investigated the effect of different N conditions in leaves of Lotus corniculatus. Low nitrogen conditions strongly enhanced the biosynthesis of phenolic compounds in leaves, inducing the expression genes encoding for key enzymes of phenolic metabolism such as phenylalanine ammonia lyase, chalcone synthase and isoflavone synthase. Kaempferol and quercetin glycosides as well as isoflavanes accumulated in leaves. Surprisingly, if the nitrogen availability was limited, but without nitrogen deprivation symptoms, an increase in the levels of phenylpropane acids and a decrease in the levels of kaempferol glycosides was detected. Especially the decrease in malonylated kaempferol derivatives and the kaempferol glycosides substituted by rhamnose in the 3-O position. The L. corniculatus malonyltransferase 1 gene is likely involved in the malonylation of the kaempferol glycosides. |
| first_indexed | 2024-03-08T16:50:57Z |
| format | Article |
| id | doaj.art-4f47a60fd525407383d7a50b292e8aa1 |
| institution | Directory Open Access Journal |
| issn | 2667-064X |
| language | English |
| last_indexed | 2024-03-08T16:50:57Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Stress |
| spelling | doaj.art-4f47a60fd525407383d7a50b292e8aa12024-01-05T04:25:22ZengElsevierPlant Stress2667-064X2024-03-0111100336Low nitrogen status affects isoflavonoid production and flavonol decoration in Lotus corniculatusKristina Trush0Martina Gavurová1María Dolores Monje-Rueda2Vladislav Kolarčik3Michaela Bačovčinová4Marco Betti5Peter Paľove-Balang6Institute of Biology and Ecology, Faculty of Science, P.J. Šafárik Unversity in Košice, Mánesova 23, SK-04001, Košice, SlovakiaInstitute of Biology and Ecology, Faculty of Science, P.J. Šafárik Unversity in Košice, Mánesova 23, SK-04001, Košice, SlovakiaDepartamento de Bioquímica Vegetal y Biología Molecular, Facultad de Química, Universidad de Sevilla, C/Professor Gárcia González 1, E-41012 Seville, SpainInstitute of Biology and Ecology, Faculty of Science, P.J. Šafárik Unversity in Košice, Mánesova 23, SK-04001, Košice, SlovakiaInstitute of Biology and Ecology, Faculty of Science, P.J. Šafárik Unversity in Košice, Mánesova 23, SK-04001, Košice, SlovakiaDepartamento de Bioquímica Vegetal y Biología Molecular, Facultad de Química, Universidad de Sevilla, C/Professor Gárcia González 1, E-41012 Seville, SpainInstitute of Biology and Ecology, Faculty of Science, P.J. Šafárik Unversity in Košice, Mánesova 23, SK-04001, Košice, Slovakia; Corresponding author.Nitrogen availability is key factor for plant metabolism and has a strong impact on the biosynthesis of secondary plant metabolites. A number of these metabolites are involved in the response to stress. In this paper, we investigated the effect of different N conditions in leaves of Lotus corniculatus. Low nitrogen conditions strongly enhanced the biosynthesis of phenolic compounds in leaves, inducing the expression genes encoding for key enzymes of phenolic metabolism such as phenylalanine ammonia lyase, chalcone synthase and isoflavone synthase. Kaempferol and quercetin glycosides as well as isoflavanes accumulated in leaves. Surprisingly, if the nitrogen availability was limited, but without nitrogen deprivation symptoms, an increase in the levels of phenylpropane acids and a decrease in the levels of kaempferol glycosides was detected. Especially the decrease in malonylated kaempferol derivatives and the kaempferol glycosides substituted by rhamnose in the 3-O position. The L. corniculatus malonyltransferase 1 gene is likely involved in the malonylation of the kaempferol glycosides.http://www.sciencedirect.com/science/article/pii/S2667064X23002038Flavonol decorationIsoflavanesLotus corniculatusMalonylationNitrogen availability |
| spellingShingle | Kristina Trush Martina Gavurová María Dolores Monje-Rueda Vladislav Kolarčik Michaela Bačovčinová Marco Betti Peter Paľove-Balang Low nitrogen status affects isoflavonoid production and flavonol decoration in Lotus corniculatus Plant Stress Flavonol decoration Isoflavanes Lotus corniculatus Malonylation Nitrogen availability |
| title | Low nitrogen status affects isoflavonoid production and flavonol decoration in Lotus corniculatus |
| title_full | Low nitrogen status affects isoflavonoid production and flavonol decoration in Lotus corniculatus |
| title_fullStr | Low nitrogen status affects isoflavonoid production and flavonol decoration in Lotus corniculatus |
| title_full_unstemmed | Low nitrogen status affects isoflavonoid production and flavonol decoration in Lotus corniculatus |
| title_short | Low nitrogen status affects isoflavonoid production and flavonol decoration in Lotus corniculatus |
| title_sort | low nitrogen status affects isoflavonoid production and flavonol decoration in lotus corniculatus |
| topic | Flavonol decoration Isoflavanes Lotus corniculatus Malonylation Nitrogen availability |
| url | http://www.sciencedirect.com/science/article/pii/S2667064X23002038 |
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