High-resolution structures of malaria parasite actomyosin and actin filaments.
Malaria is responsible for half a million deaths annually and poses a huge economic burden on the developing world. The mosquito-borne parasites (Plasmodium spp.) that cause the disease depend upon an unconventional actomyosin motor for both gliding motility and host cell invasion. The motor system,...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-04-01
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| Series: | PLoS Pathogens |
| Online Access: | https://doi.org/10.1371/journal.ppat.1010408 |
| _version_ | 1818473419114545152 |
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| author | Juha Vahokoski Lesley J Calder Andrea J Lopez Justin E Molloy Inari Kursula Peter B Rosenthal |
| author_facet | Juha Vahokoski Lesley J Calder Andrea J Lopez Justin E Molloy Inari Kursula Peter B Rosenthal |
| author_sort | Juha Vahokoski |
| collection | DOAJ |
| description | Malaria is responsible for half a million deaths annually and poses a huge economic burden on the developing world. The mosquito-borne parasites (Plasmodium spp.) that cause the disease depend upon an unconventional actomyosin motor for both gliding motility and host cell invasion. The motor system, often referred to as the glideosome complex, remains to be understood in molecular terms and is an attractive target for new drugs that might block the infection pathway. Here, we present the high-resolution structure of the actomyosin motor complex from Plasmodium falciparum. The complex includes the malaria parasite actin filament (PfAct1) complexed with the class XIV myosin motor (PfMyoA) and its two associated light-chains. The high-resolution core structure reveals the PfAct1:PfMyoA interface in atomic detail, while at lower-resolution, we visualize the PfMyoA light-chain binding region, including the essential light chain (PfELC) and the myosin tail interacting protein (PfMTIP). Finally, we report a bare PfAct1 filament structure at improved resolution. |
| first_indexed | 2024-04-14T04:23:52Z |
| format | Article |
| id | doaj.art-4f48943ad9af4905a7553855890a832d |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-14T04:23:52Z |
| publishDate | 2022-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-4f48943ad9af4905a7553855890a832d2022-12-22T02:12:24ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742022-04-01184e101040810.1371/journal.ppat.1010408High-resolution structures of malaria parasite actomyosin and actin filaments.Juha VahokoskiLesley J CalderAndrea J LopezJustin E MolloyInari KursulaPeter B RosenthalMalaria is responsible for half a million deaths annually and poses a huge economic burden on the developing world. The mosquito-borne parasites (Plasmodium spp.) that cause the disease depend upon an unconventional actomyosin motor for both gliding motility and host cell invasion. The motor system, often referred to as the glideosome complex, remains to be understood in molecular terms and is an attractive target for new drugs that might block the infection pathway. Here, we present the high-resolution structure of the actomyosin motor complex from Plasmodium falciparum. The complex includes the malaria parasite actin filament (PfAct1) complexed with the class XIV myosin motor (PfMyoA) and its two associated light-chains. The high-resolution core structure reveals the PfAct1:PfMyoA interface in atomic detail, while at lower-resolution, we visualize the PfMyoA light-chain binding region, including the essential light chain (PfELC) and the myosin tail interacting protein (PfMTIP). Finally, we report a bare PfAct1 filament structure at improved resolution.https://doi.org/10.1371/journal.ppat.1010408 |
| spellingShingle | Juha Vahokoski Lesley J Calder Andrea J Lopez Justin E Molloy Inari Kursula Peter B Rosenthal High-resolution structures of malaria parasite actomyosin and actin filaments. PLoS Pathogens |
| title | High-resolution structures of malaria parasite actomyosin and actin filaments. |
| title_full | High-resolution structures of malaria parasite actomyosin and actin filaments. |
| title_fullStr | High-resolution structures of malaria parasite actomyosin and actin filaments. |
| title_full_unstemmed | High-resolution structures of malaria parasite actomyosin and actin filaments. |
| title_short | High-resolution structures of malaria parasite actomyosin and actin filaments. |
| title_sort | high resolution structures of malaria parasite actomyosin and actin filaments |
| url | https://doi.org/10.1371/journal.ppat.1010408 |
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