The Impact of Magnetic Field and Gibberellin Treatment on the Release of Dormancy and Internal Nutrient Transformation in <i>Tilia miqueliana</i> Maxim. Seeds
The seeds of <i>Tilia miqueliana</i> Maxim. exhibit deep dormancy, which is categorized as combinational dormancy. This study utilized a comprehensive treatment involving magnetic fields, gibberellin (GA<sub>3</sub>), and cold stratification to promote the release of seed phy...
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MDPI AG
2024-02-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/15/2/311 |
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| author | Fenghou Shi Yunxiang Cao Yajun Gao Yuhou Qiu Yizeng Lu Biao Han Yongbao Shen |
| author_facet | Fenghou Shi Yunxiang Cao Yajun Gao Yuhou Qiu Yizeng Lu Biao Han Yongbao Shen |
| author_sort | Fenghou Shi |
| collection | DOAJ |
| description | The seeds of <i>Tilia miqueliana</i> Maxim. exhibit deep dormancy, which is categorized as combinational dormancy. This study utilized a comprehensive treatment involving magnetic fields, gibberellin (GA<sub>3</sub>), and cold stratification to promote the release of seed physiological dormancy and enhance germination rates. After being soaked in 98% H<sub>2</sub>SO<sub>4</sub> for 15 min, mature seeds of <i>Tilia</i> were exposed to magnetic field treatments (150 MT, 250 MT) for different durations (25 min, 45 min, 65 min, and 85 min), as well as GA<sub>3</sub> solution soaking (concentration: 0 μmol·L<sup>−1</sup>, 1443 μmol·L<sup>−1</sup>). Subsequently, cold stratification (0–5 °C) was applied to investigate the effects of these treatments on seed dormancy release and nutrient transformation. The results indicated that the comprehensive treatment involving magnetic field, GA<sub>3</sub> solution soaking, and cold stratification effectively released the physiological dormancy of <i>Tilia</i> seeds and improved germination rates. Among the treatments, M<sub>150</sub>T<sub>85</sub>G<sub>1443</sub> (magnetic field intensity: 150 MT, magnetic field treatment time: 85 min, GA<sub>3</sub> soaking concentration: 1433 μmol·L<sup>−1</sup>) exhibited the most favorable outcome. After 75 days of cold stratification following the comprehensive treatments, the germination rate of M<sub>150</sub>T<sub>85</sub>G<sub>1443</sub> seeds reached 89%. Additionally, the levels of storage substances such as starches and crude fats within the seeds decreased, while the utilization of soluble sugars and soluble proteins increased. The M<sub>150</sub>T<sub>85</sub>G<sub>1443</sub> treatment exhibited the highest degree of variation, leading to gradual increases in metabolic activities of the seeds and a transition from dormancy to germination. |
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| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-07T22:32:30Z |
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| spelling | doaj.art-4f49dded510245e9b7afc46222c5e2212024-02-23T15:16:55ZengMDPI AGForests1999-49072024-02-0115231110.3390/f15020311The Impact of Magnetic Field and Gibberellin Treatment on the Release of Dormancy and Internal Nutrient Transformation in <i>Tilia miqueliana</i> Maxim. SeedsFenghou Shi0Yunxiang Cao1Yajun Gao2Yuhou Qiu3Yizeng Lu4Biao Han5Yongbao Shen6Collaborative Innovation Centre of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaCollaborative Innovation Centre of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaCollaborative Innovation Centre of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaCollaborative Innovation Centre of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaShandong Provincial Center of Forest and Grass Germplasm Resources, Jinan 250102, ChinaShandong Provincial Center of Forest and Grass Germplasm Resources, Jinan 250102, ChinaCollaborative Innovation Centre of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, ChinaThe seeds of <i>Tilia miqueliana</i> Maxim. exhibit deep dormancy, which is categorized as combinational dormancy. This study utilized a comprehensive treatment involving magnetic fields, gibberellin (GA<sub>3</sub>), and cold stratification to promote the release of seed physiological dormancy and enhance germination rates. After being soaked in 98% H<sub>2</sub>SO<sub>4</sub> for 15 min, mature seeds of <i>Tilia</i> were exposed to magnetic field treatments (150 MT, 250 MT) for different durations (25 min, 45 min, 65 min, and 85 min), as well as GA<sub>3</sub> solution soaking (concentration: 0 μmol·L<sup>−1</sup>, 1443 μmol·L<sup>−1</sup>). Subsequently, cold stratification (0–5 °C) was applied to investigate the effects of these treatments on seed dormancy release and nutrient transformation. The results indicated that the comprehensive treatment involving magnetic field, GA<sub>3</sub> solution soaking, and cold stratification effectively released the physiological dormancy of <i>Tilia</i> seeds and improved germination rates. Among the treatments, M<sub>150</sub>T<sub>85</sub>G<sub>1443</sub> (magnetic field intensity: 150 MT, magnetic field treatment time: 85 min, GA<sub>3</sub> soaking concentration: 1433 μmol·L<sup>−1</sup>) exhibited the most favorable outcome. After 75 days of cold stratification following the comprehensive treatments, the germination rate of M<sub>150</sub>T<sub>85</sub>G<sub>1443</sub> seeds reached 89%. Additionally, the levels of storage substances such as starches and crude fats within the seeds decreased, while the utilization of soluble sugars and soluble proteins increased. The M<sub>150</sub>T<sub>85</sub>G<sub>1443</sub> treatment exhibited the highest degree of variation, leading to gradual increases in metabolic activities of the seeds and a transition from dormancy to germination.https://www.mdpi.com/1999-4907/15/2/311<i>Tilia miqueliana</i>seed dormancymagnetic fieldGA<sub>3</sub>nutrientsseed treatment |
| spellingShingle | Fenghou Shi Yunxiang Cao Yajun Gao Yuhou Qiu Yizeng Lu Biao Han Yongbao Shen The Impact of Magnetic Field and Gibberellin Treatment on the Release of Dormancy and Internal Nutrient Transformation in <i>Tilia miqueliana</i> Maxim. Seeds Forests <i>Tilia miqueliana</i> seed dormancy magnetic field GA<sub>3</sub> nutrients seed treatment |
| title | The Impact of Magnetic Field and Gibberellin Treatment on the Release of Dormancy and Internal Nutrient Transformation in <i>Tilia miqueliana</i> Maxim. Seeds |
| title_full | The Impact of Magnetic Field and Gibberellin Treatment on the Release of Dormancy and Internal Nutrient Transformation in <i>Tilia miqueliana</i> Maxim. Seeds |
| title_fullStr | The Impact of Magnetic Field and Gibberellin Treatment on the Release of Dormancy and Internal Nutrient Transformation in <i>Tilia miqueliana</i> Maxim. Seeds |
| title_full_unstemmed | The Impact of Magnetic Field and Gibberellin Treatment on the Release of Dormancy and Internal Nutrient Transformation in <i>Tilia miqueliana</i> Maxim. Seeds |
| title_short | The Impact of Magnetic Field and Gibberellin Treatment on the Release of Dormancy and Internal Nutrient Transformation in <i>Tilia miqueliana</i> Maxim. Seeds |
| title_sort | impact of magnetic field and gibberellin treatment on the release of dormancy and internal nutrient transformation in i tilia miqueliana i maxim seeds |
| topic | <i>Tilia miqueliana</i> seed dormancy magnetic field GA<sub>3</sub> nutrients seed treatment |
| url | https://www.mdpi.com/1999-4907/15/2/311 |
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