Expression Analysis of Five Different Long Non-Coding Ribonucleic Acids in Nonsmall-Cell Lung Carcinoma Tumor and Tumor-Derived Exosomes
Long non-coding ribonucleic acids (LncRNAs) are recently known for their role in regulating gene expression and the development of cancer. Controversial results indicate a correlation between the tissue expression of LncRNA and LncRNA content of extracellular vesicles. The present study aimed to eva...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/12/12/3209 |
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| author | Samaneh Talebi Asal Jalal Abadi Golnesa Kazemioula Nayyerehalsadat Hosseini Forough Taheri Saba Pourali Touba Mahdloo Marzieh Rezaei Mohammadreza Mirinezhad Naser Ajami Arash Salmaninejad |
| author_facet | Samaneh Talebi Asal Jalal Abadi Golnesa Kazemioula Nayyerehalsadat Hosseini Forough Taheri Saba Pourali Touba Mahdloo Marzieh Rezaei Mohammadreza Mirinezhad Naser Ajami Arash Salmaninejad |
| author_sort | Samaneh Talebi |
| collection | DOAJ |
| description | Long non-coding ribonucleic acids (LncRNAs) are recently known for their role in regulating gene expression and the development of cancer. Controversial results indicate a correlation between the tissue expression of LncRNA and LncRNA content of extracellular vesicles. The present study aimed to evaluate the expression of different LncRNAs in non-small cell lung cancer (NSCLC) patients in tumor tissue, adjacent non-cancerous tissue (ANCT), and exosome-mediated lncRNA. Tumor and ANCT, as well as serum samples of 168 patient with NSCLC, were collected. The GHSROS, HNF1A-AS1, HOTAIR, HMlincRNA717, and LINCRNA-p21 relative expressions in tumor tissue, ANCT, and serum exosomes were evaluated in NSCLC patients. Among 168 NSCLC samples, the expressions of GHSROS (REx = 3.64, <i>p</i> = 0.028), HNF1A-AS1 (REx = 2.97, <i>p</i> = 0.041), and HOTAIR (REx = 2.9, <i>p</i> = 0.0389) were upregulated, and the expressions of HMlincRNA717 (REx = −4.56, <i>p</i> = 0.0012) and LINCRNA-p21 (REx = −5.14, <i>p</i> = 0.00334) were downregulated in tumor tissue in contrast to ANCT. Moreover, similar statistical differences were seen in the exosome-derived RNA of tumor tissues in contrast to ANCT samples. A panel of the five lncRNAs demonstrated that the area under the curve (AUC) for exosome and tumor was 0.937 (standard error: 0.012, <i>p</i> value < 0.0001). LncRNAs GHSROS, HNF1A-AS1, and HOTAIR showed high expression in tumor tissue and exosome content in NSCLC, and a panel that consisted of all five lncRNAs improved diagnosis of NSCLC. |
| first_indexed | 2024-03-09T17:08:15Z |
| format | Article |
| id | doaj.art-4f4a84707db24d44bdba35a27bac672d |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-09T17:08:15Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-4f4a84707db24d44bdba35a27bac672d2023-11-24T14:20:39ZengMDPI AGDiagnostics2075-44182022-12-011212320910.3390/diagnostics12123209Expression Analysis of Five Different Long Non-Coding Ribonucleic Acids in Nonsmall-Cell Lung Carcinoma Tumor and Tumor-Derived ExosomesSamaneh Talebi0Asal Jalal Abadi1Golnesa Kazemioula2Nayyerehalsadat Hosseini3Forough Taheri4Saba Pourali5Touba Mahdloo6Marzieh Rezaei7Mohammadreza Mirinezhad8Naser Ajami9Arash Salmaninejad10Department of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177899191, IranDepartment of Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran 1916893813, IranDepartment of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran 917852689536, IranDepartment of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177899191, IranDepartment of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Science, Isfahan 9177856225, IranDepartment of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177899191, IranDepartment of Science, Kish International Campus, University of Tehran, Kish 9177856228, IranDepartment of Medical Biotechnology & Nanotechnology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177899191, IranDepartment of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177899191, IranDepartment of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177899191, IranDepartment of Medical Genetics, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad 9177899191, IranLong non-coding ribonucleic acids (LncRNAs) are recently known for their role in regulating gene expression and the development of cancer. Controversial results indicate a correlation between the tissue expression of LncRNA and LncRNA content of extracellular vesicles. The present study aimed to evaluate the expression of different LncRNAs in non-small cell lung cancer (NSCLC) patients in tumor tissue, adjacent non-cancerous tissue (ANCT), and exosome-mediated lncRNA. Tumor and ANCT, as well as serum samples of 168 patient with NSCLC, were collected. The GHSROS, HNF1A-AS1, HOTAIR, HMlincRNA717, and LINCRNA-p21 relative expressions in tumor tissue, ANCT, and serum exosomes were evaluated in NSCLC patients. Among 168 NSCLC samples, the expressions of GHSROS (REx = 3.64, <i>p</i> = 0.028), HNF1A-AS1 (REx = 2.97, <i>p</i> = 0.041), and HOTAIR (REx = 2.9, <i>p</i> = 0.0389) were upregulated, and the expressions of HMlincRNA717 (REx = −4.56, <i>p</i> = 0.0012) and LINCRNA-p21 (REx = −5.14, <i>p</i> = 0.00334) were downregulated in tumor tissue in contrast to ANCT. Moreover, similar statistical differences were seen in the exosome-derived RNA of tumor tissues in contrast to ANCT samples. A panel of the five lncRNAs demonstrated that the area under the curve (AUC) for exosome and tumor was 0.937 (standard error: 0.012, <i>p</i> value < 0.0001). LncRNAs GHSROS, HNF1A-AS1, and HOTAIR showed high expression in tumor tissue and exosome content in NSCLC, and a panel that consisted of all five lncRNAs improved diagnosis of NSCLC.https://www.mdpi.com/2075-4418/12/12/3209nonsmall-cell lung carcinomaexosomeslong non-coding ribonucleic acidsexpression |
| spellingShingle | Samaneh Talebi Asal Jalal Abadi Golnesa Kazemioula Nayyerehalsadat Hosseini Forough Taheri Saba Pourali Touba Mahdloo Marzieh Rezaei Mohammadreza Mirinezhad Naser Ajami Arash Salmaninejad Expression Analysis of Five Different Long Non-Coding Ribonucleic Acids in Nonsmall-Cell Lung Carcinoma Tumor and Tumor-Derived Exosomes Diagnostics nonsmall-cell lung carcinoma exosomes long non-coding ribonucleic acids expression |
| title | Expression Analysis of Five Different Long Non-Coding Ribonucleic Acids in Nonsmall-Cell Lung Carcinoma Tumor and Tumor-Derived Exosomes |
| title_full | Expression Analysis of Five Different Long Non-Coding Ribonucleic Acids in Nonsmall-Cell Lung Carcinoma Tumor and Tumor-Derived Exosomes |
| title_fullStr | Expression Analysis of Five Different Long Non-Coding Ribonucleic Acids in Nonsmall-Cell Lung Carcinoma Tumor and Tumor-Derived Exosomes |
| title_full_unstemmed | Expression Analysis of Five Different Long Non-Coding Ribonucleic Acids in Nonsmall-Cell Lung Carcinoma Tumor and Tumor-Derived Exosomes |
| title_short | Expression Analysis of Five Different Long Non-Coding Ribonucleic Acids in Nonsmall-Cell Lung Carcinoma Tumor and Tumor-Derived Exosomes |
| title_sort | expression analysis of five different long non coding ribonucleic acids in nonsmall cell lung carcinoma tumor and tumor derived exosomes |
| topic | nonsmall-cell lung carcinoma exosomes long non-coding ribonucleic acids expression |
| url | https://www.mdpi.com/2075-4418/12/12/3209 |
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