Comparative Genomic and Transcriptomic Analyses of Mycobacterium kansasii Subtypes Provide New Insights Into Their Pathogenicity and Taxonomy
Mycobacterium kansasii is an important opportunistic pathogen of humans and has a close phylogenetic relationship with Mycobacterium tuberculosis. Seven subtypes (I–VII) have been identified using molecular biology approaches, of which subtype I is the most frequent causative agent of human disease....
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2020.00122/full |
| _version_ | 1819150290557861888 |
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| author | Qingtian Guan Roy Ummels Fathia Ben-Rached Yara Alzahid Mohammad S. Amini Sabir A. Adroub Jakko van Ingen Wilbert Bitter Abdallah M. Abdallah Abdallah M. Abdallah Arnab Pain Arnab Pain |
| author_facet | Qingtian Guan Roy Ummels Fathia Ben-Rached Yara Alzahid Mohammad S. Amini Sabir A. Adroub Jakko van Ingen Wilbert Bitter Abdallah M. Abdallah Abdallah M. Abdallah Arnab Pain Arnab Pain |
| author_sort | Qingtian Guan |
| collection | DOAJ |
| description | Mycobacterium kansasii is an important opportunistic pathogen of humans and has a close phylogenetic relationship with Mycobacterium tuberculosis. Seven subtypes (I–VII) have been identified using molecular biology approaches, of which subtype I is the most frequent causative agent of human disease. To investigate the genotypes and pathogenic components of M. kansasii, we sequenced and compared the complete base-perfect genomes of different M. kansasii subtypes. Our findings support the proposition that M. kansasii “subtypes” I-VI, whose assemblies are currently available, should be considered as different species. Furthermore, we identified the exclusive presence of the espACD operon in M. kansasii subtype I, and we confirmed its role in the pathogenicity of M. kansasii in a cell infection model. The espACD operon is exclusively present in mycobacterial species that induce phagosomal rupture in host phagocytes and is known to be a major determinant of ESX1-mediated virulence in pathogenic mycobacteria. Comparative transcriptome analysis of the M. kansasii I-V strains identified genes potentially associated with virulence. Using a comparative genomics approach, we designed primers for PCR genotyping of M. kansasii subtypes I-V and tested their efficacy using clinically relevant strains of M. kansasii. |
| first_indexed | 2024-12-22T14:15:09Z |
| format | Article |
| id | doaj.art-4f4e5c3d72774c9b921df1666201ab4d |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-22T14:15:09Z |
| publishDate | 2020-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-4f4e5c3d72774c9b921df1666201ab4d2022-12-21T18:23:08ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-03-011010.3389/fcimb.2020.00122513548Comparative Genomic and Transcriptomic Analyses of Mycobacterium kansasii Subtypes Provide New Insights Into Their Pathogenicity and TaxonomyQingtian Guan0Roy Ummels1Fathia Ben-Rached2Yara Alzahid3Mohammad S. Amini4Sabir A. Adroub5Jakko van Ingen6Wilbert Bitter7Abdallah M. Abdallah8Abdallah M. Abdallah9Arnab Pain10Arnab Pain11Pathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDepartment of Medical Microbiology and Infection Control, Amsterdam University Medical Centers, Amsterdam, NetherlandsPathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaPathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaPathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaPathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaDepartment of Medical Microbiology, Radboud UMC Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, NetherlandsDepartment of Medical Microbiology and Infection Control, Amsterdam University Medical Centers, Amsterdam, NetherlandsDepartment of Basic Medical Sciences, College of Medicine, QU Health, Qatar University, Doha, QatarPathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaPathogen Genomics Laboratory, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi ArabiaCenter for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, Sapporo, JapanMycobacterium kansasii is an important opportunistic pathogen of humans and has a close phylogenetic relationship with Mycobacterium tuberculosis. Seven subtypes (I–VII) have been identified using molecular biology approaches, of which subtype I is the most frequent causative agent of human disease. To investigate the genotypes and pathogenic components of M. kansasii, we sequenced and compared the complete base-perfect genomes of different M. kansasii subtypes. Our findings support the proposition that M. kansasii “subtypes” I-VI, whose assemblies are currently available, should be considered as different species. Furthermore, we identified the exclusive presence of the espACD operon in M. kansasii subtype I, and we confirmed its role in the pathogenicity of M. kansasii in a cell infection model. The espACD operon is exclusively present in mycobacterial species that induce phagosomal rupture in host phagocytes and is known to be a major determinant of ESX1-mediated virulence in pathogenic mycobacteria. Comparative transcriptome analysis of the M. kansasii I-V strains identified genes potentially associated with virulence. Using a comparative genomics approach, we designed primers for PCR genotyping of M. kansasii subtypes I-V and tested their efficacy using clinically relevant strains of M. kansasii.https://www.frontiersin.org/article/10.3389/fcimb.2020.00122/fullnon-tuberculous mycobacteriacomparative genomicsM. kansasii subtypesespACD operonvirulence factor |
| spellingShingle | Qingtian Guan Roy Ummels Fathia Ben-Rached Yara Alzahid Mohammad S. Amini Sabir A. Adroub Jakko van Ingen Wilbert Bitter Abdallah M. Abdallah Abdallah M. Abdallah Arnab Pain Arnab Pain Comparative Genomic and Transcriptomic Analyses of Mycobacterium kansasii Subtypes Provide New Insights Into Their Pathogenicity and Taxonomy Frontiers in Cellular and Infection Microbiology non-tuberculous mycobacteria comparative genomics M. kansasii subtypes espACD operon virulence factor |
| title | Comparative Genomic and Transcriptomic Analyses of Mycobacterium kansasii Subtypes Provide New Insights Into Their Pathogenicity and Taxonomy |
| title_full | Comparative Genomic and Transcriptomic Analyses of Mycobacterium kansasii Subtypes Provide New Insights Into Their Pathogenicity and Taxonomy |
| title_fullStr | Comparative Genomic and Transcriptomic Analyses of Mycobacterium kansasii Subtypes Provide New Insights Into Their Pathogenicity and Taxonomy |
| title_full_unstemmed | Comparative Genomic and Transcriptomic Analyses of Mycobacterium kansasii Subtypes Provide New Insights Into Their Pathogenicity and Taxonomy |
| title_short | Comparative Genomic and Transcriptomic Analyses of Mycobacterium kansasii Subtypes Provide New Insights Into Their Pathogenicity and Taxonomy |
| title_sort | comparative genomic and transcriptomic analyses of mycobacterium kansasii subtypes provide new insights into their pathogenicity and taxonomy |
| topic | non-tuberculous mycobacteria comparative genomics M. kansasii subtypes espACD operon virulence factor |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2020.00122/full |
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