Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of <i>Eucheuma denticulatum</i>, a Tropical Red Seaweed
Seaweed is a sustainable source of marine oligosaccharides that potentially could be used as a prebiotic ingredient for functional food development. The study aims to optimize the oligosaccharide preparation through thermal hydrolysis of an under-utilized red seaweed, <i>Eucheuma denticulatum&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/13/3/1517 |
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| author | Birdie Scott Padam Chee Kiong Siew Fook Yee Chye |
| author_facet | Birdie Scott Padam Chee Kiong Siew Fook Yee Chye |
| author_sort | Birdie Scott Padam |
| collection | DOAJ |
| description | Seaweed is a sustainable source of marine oligosaccharides that potentially could be used as a prebiotic ingredient for functional food development. The study aims to optimize the oligosaccharide preparation through thermal hydrolysis of an under-utilized red seaweed, <i>Eucheuma denticulatum</i>. Response surface methodology (RSM) applying Box–Behnken design (BBD) was used on three parameters including temperature (105–135 °C), hydrolysis time (15–35 min) and sulfuric acid concentration (0.05–0.2 M). Optimized fractions with good prebiotic activity were characterized using high-performance size-exclusion chromatography (HP-SEC) and Fourier transform infrared spectroscopy (FT-IR). <i>Eucheuma denticulatum</i> oligosaccharides fraction 1 (ED-F1) was shown to promote the growth of beneficial gut microbiota including <i>Lactobacillus plantarum</i>, <i>L. casei, L. acidophilus</i>, <i>Bifidobacterium animalis</i> and <i>B. longum</i> with the highest prebiotic activity score of 1.64 ± 0.17. The optimization studies showed that hydrolysis time was the most significant parameter for the oligosaccharides yield. Optimal hydrolysis conditions for ED-F1 were 120 °C, 21 min, 0.12 M H<sub>2</sub>SO<sub>4</sub> with the highest yield achieved (11.15 g/100 g of dry weight). The molecular weight of ED-F1 was determined at 1025 Da while FT-IR analysis revealed the presence of sulfated oligosaccharides with similar characteristics of <i>i</i>-carrageenan. These findings signify the innovative method for the efficient production of seaweed derived prebiotic oligosaccharides, which could be a promising source of functional food ingredients for the development of health foods and beverages. |
| first_indexed | 2024-03-11T09:52:54Z |
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| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-03-11T09:52:54Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-4f4f67f3aded45d68a711a5e8b9bd50f2023-11-16T16:06:40ZengMDPI AGApplied Sciences2076-34172023-01-01133151710.3390/app13031517Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of <i>Eucheuma denticulatum</i>, a Tropical Red SeaweedBirdie Scott Padam0Chee Kiong Siew1Fook Yee Chye2Faculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, MalaysiaFaculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, MalaysiaFaculty of Food Science and Nutrition, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu 88400, Sabah, MalaysiaSeaweed is a sustainable source of marine oligosaccharides that potentially could be used as a prebiotic ingredient for functional food development. The study aims to optimize the oligosaccharide preparation through thermal hydrolysis of an under-utilized red seaweed, <i>Eucheuma denticulatum</i>. Response surface methodology (RSM) applying Box–Behnken design (BBD) was used on three parameters including temperature (105–135 °C), hydrolysis time (15–35 min) and sulfuric acid concentration (0.05–0.2 M). Optimized fractions with good prebiotic activity were characterized using high-performance size-exclusion chromatography (HP-SEC) and Fourier transform infrared spectroscopy (FT-IR). <i>Eucheuma denticulatum</i> oligosaccharides fraction 1 (ED-F1) was shown to promote the growth of beneficial gut microbiota including <i>Lactobacillus plantarum</i>, <i>L. casei, L. acidophilus</i>, <i>Bifidobacterium animalis</i> and <i>B. longum</i> with the highest prebiotic activity score of 1.64 ± 0.17. The optimization studies showed that hydrolysis time was the most significant parameter for the oligosaccharides yield. Optimal hydrolysis conditions for ED-F1 were 120 °C, 21 min, 0.12 M H<sub>2</sub>SO<sub>4</sub> with the highest yield achieved (11.15 g/100 g of dry weight). The molecular weight of ED-F1 was determined at 1025 Da while FT-IR analysis revealed the presence of sulfated oligosaccharides with similar characteristics of <i>i</i>-carrageenan. These findings signify the innovative method for the efficient production of seaweed derived prebiotic oligosaccharides, which could be a promising source of functional food ingredients for the development of health foods and beverages.https://www.mdpi.com/2076-3417/13/3/1517optimizationresponse surface methodologylactobacillusbifidobacterium<i>Eucheuma denticulatum</i>seaweed oligosaccharide |
| spellingShingle | Birdie Scott Padam Chee Kiong Siew Fook Yee Chye Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of <i>Eucheuma denticulatum</i>, a Tropical Red Seaweed Applied Sciences optimization response surface methodology lactobacillus bifidobacterium <i>Eucheuma denticulatum</i> seaweed oligosaccharide |
| title | Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of <i>Eucheuma denticulatum</i>, a Tropical Red Seaweed |
| title_full | Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of <i>Eucheuma denticulatum</i>, a Tropical Red Seaweed |
| title_fullStr | Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of <i>Eucheuma denticulatum</i>, a Tropical Red Seaweed |
| title_full_unstemmed | Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of <i>Eucheuma denticulatum</i>, a Tropical Red Seaweed |
| title_short | Optimization of an Innovative Hydrothermal Processing on Prebiotic Properties of <i>Eucheuma denticulatum</i>, a Tropical Red Seaweed |
| title_sort | optimization of an innovative hydrothermal processing on prebiotic properties of i eucheuma denticulatum i a tropical red seaweed |
| topic | optimization response surface methodology lactobacillus bifidobacterium <i>Eucheuma denticulatum</i> seaweed oligosaccharide |
| url | https://www.mdpi.com/2076-3417/13/3/1517 |
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